{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:05:13Z","timestamp":1774314313371,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001862","name":"FORMAS","doi-asserted-by":"publisher","award":["2021-02262"],"award-info":[{"award-number":["2021-02262"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001862","name":"FORMAS","doi-asserted-by":"publisher","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001862","name":"FORMAS","doi-asserted-by":"publisher","award":["UIDP\/04326\/2020"],"award-info":[{"award-number":["UIDP\/04326\/2020"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001862","name":"FORMAS","doi-asserted-by":"publisher","award":["LA\/P\/0101\/2020"],"award-info":[{"award-number":["LA\/P\/0101\/2020"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Portuguese national funds from FCT\u2014Foundation for Science and Technology","award":["2021-02262"],"award-info":[{"award-number":["2021-02262"]}]},{"name":"Portuguese national funds from FCT\u2014Foundation for Science and Technology","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}]},{"name":"Portuguese national funds from FCT\u2014Foundation for Science and Technology","award":["UIDP\/04326\/2020"],"award-info":[{"award-number":["UIDP\/04326\/2020"]}]},{"name":"Portuguese national funds from FCT\u2014Foundation for Science and Technology","award":["LA\/P\/0101\/2020"],"award-info":[{"award-number":["LA\/P\/0101\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Nociceptors are receptors that detect injurious stimuli and are necessary to convey such information from the periphery to the central nervous system. While nociception has been extensively studied in various taxa, there is relatively little electrophysiological evidence for the existence of nociceptors in decapod crustaceans. This study investigated putative nociceptive responses in the shore crabs, specifically their response to mechanical and noxious chemical stimuli. Extracellular multi-unit electrophysiological recordings were conducted from the anterior ganglion and the circumesophageal connective ganglia to assess nociceptive responses. Soft tissues at the joints of the chelae, antennae, and walking legs were stimulated using acetic acid (noxious stimulus) and von Frey hairs (mechanical stimulus), while nearby ganglion activity was recorded. The results indicate the existence of nociceptors in the tested areas, with mechanical stimuli eliciting shorter, more intense neural activity compared with acetic acid. Although acetic acid triggered responses in all areas, the antennae and antennules did not respond to mechanical stimuli. Though we acknowledge the challenges of conducting in vivo electrophysiological recordings, future research should focus on further characterizing nociceptor activity because the results suggest the presence of nociceptors.<\/jats:p>","DOI":"10.3390\/biology13110851","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T11:31:57Z","timestamp":1729596717000},"page":"851","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Putative Nociceptive Responses in a Decapod Crustacean: The Shore Crab (Carcinus maenas)"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3606-8049","authenticated-orcid":false,"given":"Eleftherios","family":"Kasiouras","sequence":"first","affiliation":[{"name":"Department of Biological and Environmental Sciences, University of Gothenburg, P.O. Box 463, 405 31 Gothenburg, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3007-4647","authenticated-orcid":false,"given":"Peter C.","family":"Hubbard","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0268-1199","authenticated-orcid":false,"given":"Albin","family":"Gr\u00e4ns","sequence":"additional","affiliation":[{"name":"Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences, P.O. Box 463, 405 31 Gothenburg, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9787-3948","authenticated-orcid":false,"given":"Lynne U.","family":"Sneddon","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences, University of Gothenburg, P.O. Box 463, 405 31 Gothenburg, Sweden"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.beproc.2016.06.017","article-title":"Trade-offs between predator avoidance and electric shock avoidance in hermit crabs demonstrate a non-reflexive response to noxious stimuli consistent with prediction of pain","volume":"130","author":"Magee","year":"2016","journal-title":"Behav. Process."},{"key":"ref_2","unstructured":"Birch, J., Burn, C., Schnell, A., Browning, H., and Crump, A. (2024, October 13). Review of the Evidence of Sentience in Cephalopod Molluscs and Decapod Crustaceans, LSE Consulting. LSE Enterprise Ltd. The London School of Economics and Political Science. Available online: https:\/\/www.lse.ac.uk\/News\/News-Assets\/PDFs\/2021\/Sentience-in-Cephalopod-Molluscs-and-Decapod-Crustaceans-Final-Report-November-2021.pdf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.anbehav.2007.07.004","article-title":"Nociception or pain in a decapod crustacean?","volume":"75","author":"Barr","year":"2008","journal-title":"Anim. Behav."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.neuron.2015.06.005","article-title":"Nociception, Pain, Negative Moods, and Behavior Selection","volume":"87","author":"Baliki","year":"2015","journal-title":"Neuron"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.anbehav.2014.09.007","article-title":"Defining and assessing animal pain","volume":"97","author":"Sneddon","year":"2014","journal-title":"Anim. Behav."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/0361-9230(88)90030-5","article-title":"Evolutionary and comparative aspects of nociception","volume":"21","author":"Kavaliers","year":"1988","journal-title":"Brain Res. Bull."},{"key":"ref_7","unstructured":"Sherrington, C. (1906). The Integrative Action of the Nervous System, Oxford University Press. [1st ed.]."},{"key":"ref_8","unstructured":"Derderian, C., Shumway, K.R., and Prasanna, T. (2024, August 18). Physiology, Withdrawal Response, StatPearls, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK544292\/."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S103","DOI":"10.1017\/S0962728600023551","article-title":"Can Invertebrates Suffer? or, How Robust is Argument-By-Analogy?","volume":"10","author":"Sherwin","year":"2001","journal-title":"Anim. Welf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/S0003-3472(05)80127-7","article-title":"Assessment of pain in animals","volume":"42","author":"Bateson","year":"1991","journal-title":"Anim. Behav."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1007\/s00359-009-0482-z","article-title":"Nociceptors: A phylogenetic view","volume":"195","author":"Smith","year":"2009","journal-title":"J. Comp. Physiol. A"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.7120\/096272812X13353700593365","article-title":"Evidence for pain in decapod crustaceans","volume":"21","author":"Elwood","year":"2012","journal-title":"Anim. Welf."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.beproc.2017.03.022","article-title":"Aversive responses by shore crabs to acetic acid but not to capsaicin","volume":"140","author":"Elwood","year":"2017","journal-title":"Behav. Process."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Barr, S. (2024). and Elwood. R.W. Trade-Offs between Avoidance of Noxious Electric Shock and Avoidance of Bright Light in Shore Crabs Are Consistent with Predictions of Pain. Animals, 14.","DOI":"10.3390\/ani14050770"},{"key":"ref_15","first-page":"441","article-title":"Can crayfish take the heat?","volume":"4","author":"Puri","year":"2015","journal-title":"Procambarus clarkii show nociceptive behaviour to high temperature stimuli, but not low temperature or chemical stimuli. Biol. Open"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Puri, S., and Faulkes, Z. (2010). Do Decapod Crustaceans Have Nociceptors for Extreme pH?. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0010244"},{"key":"ref_17","unstructured":"Purves, D., and Williams, S.M. (2001). Neuroscience, Sinauer Associates. [2nd ed.]."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Watling, L., and Thiel, M. (2013). Structure of the Nervous System. Functional Morphology and Diversity, Oxford University Press.","DOI":"10.1093\/acprof:osobl\/9780195398038.001.0001"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e5634","DOI":"10.7717\/peerj.5634","article-title":"Functional responses of a cosmopolitan invader demonstrate intraspecific variability in consumer-resource dynamics","volume":"6","author":"Howard","year":"2018","journal-title":"PeerJ"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.envint.2014.05.018","article-title":"The crab Carcinus maenas as a suitable experimental model in ecotoxicology","volume":"70","author":"Rodrigues","year":"2014","journal-title":"Environ. Int."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"14","DOI":"10.3923\/jpt.2022.14.27","article-title":"Effect of TEA and 4-AP on Primary Sensory Neurons in a Crustacean Model","volume":"17","author":"Tanner","year":"2022","journal-title":"J. Pharmacol. Toxicol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/BF00324900","article-title":"The organization of the cerebral ganglion in the shore crab, Carcinus maenas: II. The relation of intracerebral blood vessels to other brain elements","volume":"120","author":"Abbott","year":"1971","journal-title":"Z. Zellforsch"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s00441-012-1353-4","article-title":"Comparative brain architecture of the European shore crab Carcinus maenas (Brachyura) and the common hermit crab Pagurus bernhardus (Anomura) with notes on other marine hermit crabs","volume":"348","author":"Krieger","year":"2012","journal-title":"Cell Tissue Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"jeb.246448","DOI":"10.1242\/jeb.246448","article-title":"Environmental salinity modulates olfactory sensitivity in the euryhaline European seabass, Dicentrarchus labrax, acclimated to seawater and brackish water","volume":"227","author":"Velez","year":"2024","journal-title":"J. Exp. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.brainresrev.2004.07.007","article-title":"Evolution of nociception in vertebrates: Comparative analysis of lower vertebrates","volume":"46","author":"Sneddon","year":"2004","journal-title":"Brain Res. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/0024-3205(92)90167-N","article-title":"Alternatives to the use of mammals for pain research","volume":"50","author":"Stevens","year":"1999","journal-title":"Life Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1017\/S0962728600015189","article-title":"Pain in Birds","volume":"1","author":"Gentle","year":"1992","journal-title":"Anim. Welf."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3760","DOI":"10.1172\/JCI42843","article-title":"Nociceptors: The sensors of the pain pathway","volume":"120","author":"Dubin","year":"2010","journal-title":"J. Clin. Investig."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.neuron.2007.07.016","article-title":"Nociceptors\u2014Noxious Stimulus Detectors","volume":"55","author":"Woolf","year":"2007","journal-title":"Neuron"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"20190290","DOI":"10.1098\/rstb.2019.0290","article-title":"Evolution of nociception and pain: Evidence from fish models","volume":"374","author":"Sneddon","year":"2019","journal-title":"Phil. Trans. R. Soc. B"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1111\/j.1460-9568.1995.tb00339.x","article-title":"The Classification and Properties of Nociceptive Afferent Units from the Skin of the Anaesthetized Pig","volume":"7","author":"Lynn","year":"1995","journal-title":"Eur. J. Neurosci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jembe.2009.06.022","article-title":"The Geography of Crushing: Variation in Claw Performance of the Invasive Crab Carcinus Maenas","volume":"377","author":"Taylor","year":"2009","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Luque, J., Allison, W.T., Bracken-Grissom, H.D., Jenkins, K.M., Palmer, A.R., Porter, M.L., and Wolfe, J.M. (2019). Evolution of crab eye structures and the utility of ommatidia morphology in resolving phylogeny. bioRxiv.","DOI":"10.1101\/786087"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1086\/713537","article-title":"The Crustacean Antennule: A Complex Organ Adapted for Lifelong Function in Diverse Environments and Lifestyles","volume":"240","author":"Derby","year":"2021","journal-title":"Biol. Bull."},{"key":"ref_35","unstructured":"Armstrong, S.A., and Herr, M.J. (2024, August 26). Physiology, Nociception, StatPearls, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK551562\/."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Di Maio, G., Villano, I., Ilardi, C.R., Messina, A., Monda, V., Iodice, A.C., Porro, C., Panaro, M.A., Chieffi, S., and Messina, G. (2023). Mechanisms of Transmission and Processing of Pain: A Narrative Review. IJERPH, 20.","DOI":"10.3390\/ijerph20043064"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1152\/jn.00324.2003","article-title":"Characterization of Cutaneous Primary Afferent Fibers Excited by Acetic Acid in a Model of Nociception in Frogs","volume":"90","author":"Hamamoto","year":"2003","journal-title":"J. Neurophysiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1080\/23308249.2023.2257802","article-title":"Reasons to Be Skeptical about Sentience and Pain in Fishes and Aquatic Invertebrates","volume":"32","author":"Diggles","year":"2024","journal-title":"Rev. Fish. Sci. Aquac."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"9075","DOI":"10.1073\/pnas.0901507106","article-title":"Distinct Subsets of Unmyelinated Primary Sensory Fibers Mediate Behavioral Responses to Noxious Thermal and Mechanical Stimuli","volume":"106","author":"Cavanaugh","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1152\/physiol.00022.2017","article-title":"Comparative Physiology of Nociception and Pain","volume":"33","author":"Sneddon","year":"2018","journal-title":"Physiology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.brainres.2007.07.011","article-title":"Nociception in fish: Stimulus\u2013response properties of receptors on the head of trout Oncorhynchus mykiss","volume":"1166","author":"Ashley","year":"2007","journal-title":"Brain Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1242\/jeb.060350","article-title":"Characterisation of chemosensory trigeminal receptors in the rainbow trout, Oncorhynchus mykiss: Responses to chemical irritants and carbon dioxide","volume":"215","author":"Mettam","year":"2012","journal-title":"J. Exp. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s004410100377","article-title":"Morphology and Distribution of Setae on the Antennules of the Caribbean Spiny Lobster Panulirus Argus Reveal New Types of Bimodal Chemo-Mechanosensilla","volume":"304","author":"Cate","year":"2001","journal-title":"Cell Tissue Res."}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/13\/11\/851\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:17:54Z","timestamp":1760113074000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/13\/11\/851"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,22]]},"references-count":43,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["biology13110851"],"URL":"https:\/\/doi.org\/10.3390\/biology13110851","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,22]]}}}