{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T08:16:41Z","timestamp":1774513001992,"version":"3.50.1"},"reference-count":80,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T00:00:00Z","timestamp":1600387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"INTERREG VA POCTEP","award":["055 ALGARED_PLUS_5E"],"award-info":[{"award-number":["055 ALGARED_PLUS_5E"]}]},{"name":"SUBV. COOP.ALENTEJO-ALGARVE-ANDALUCIA","award":["2019"],"award-info":[{"award-number":["2019"]}]},{"DOI":"10.13039\/501100005604","name":"University of Huelva","doi-asserted-by":"publisher","award":["EPIT 2016-17"],"award-info":[{"award-number":["EPIT 2016-17"]}],"id":[{"id":"10.13039\/501100005604","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>The need to survive in extreme environments has furnished haloarchaea with a series of components specially adapted to work in such conditions. The possible application of these molecules in the pharmaceutical and industrial fields has received increasing attention; however, many potential bioactivities of haloarchaea are still poorly explored. In this paper, we describe the isolation and identification of two new haloarchaeal strains from the saltern ponds located in the marshlands of the Odiel River, in the southwest of Spain, as well as the in vitro assessment of their antioxidant, antimicrobial, and bioactive properties. The acetone extract obtained from the new isolated Haloarcula strain exhibited the highest antioxidant activity, while the acetone extracts from both isolated strains demonstrated a strong antimicrobial activity, especially against other halophilic microorganisms. Moreover, these extracts showed a remarkable ability to inhibit the enzyme cyclooxygenase-2 and to activate the melanogenic enzyme tyrosinase, indicating their potential against chronic inflammation and skin pigmentation disorders. Finally, the aqueous protein-rich extracts obtained from both haloarchaea exhibited an important inhibitory effect on the activity of the acetylcholinesterase enzyme, involved in the hydrolysis of cholinergic neurotransmitters and related to several neurological diseases.<\/jats:p>","DOI":"10.3390\/biology9090298","type":"journal-article","created":{"date-parts":[[2020,9,19]],"date-time":"2020-09-19T07:06:09Z","timestamp":1600499169000},"page":"298","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain)"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0511-8724","authenticated-orcid":false,"given":"Patricia","family":"G\u00f3mez-Villegas","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry, Department of Chemistry, University of Huelva, Avda. de las Fuerzas Armadas s\/n, 21071 Huelva, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2617-5410","authenticated-orcid":false,"given":"Javier","family":"Vigara","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Chemistry, University of Huelva, Avda. de las Fuerzas Armadas s\/n, 21071 Huelva, Spain"}]},{"given":"Marta","family":"Vila","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Chemistry, University of Huelva, Avda. de las Fuerzas Armadas s\/n, 21071 Huelva, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3101-693X","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Varela","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus of Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Lu\u00edsa","family":"Barreira","sequence":"additional","affiliation":[{"name":"Centre of Marine Sciences, University of Algarve, Campus of Gambelas, 8005-139 Faro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6243-6454","authenticated-orcid":false,"given":"Rosa","family":"L\u00e9on","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Chemistry, University of Huelva, Avda. de las Fuerzas Armadas s\/n, 21071 Huelva, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.copbio.2015.02.005","article-title":"Halophilic microbial communities and their environments","volume":"33","author":"Oren","year":"2015","journal-title":"Curr. 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