{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T09:04:41Z","timestamp":1775034281998,"version":"3.50.1"},"reference-count":93,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,6]],"date-time":"2020-06-06T00:00:00Z","timestamp":1591401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BBB-BQB\/6071\/2014, UID\/Multi\/00612\/2019, UIDB\/00100\/2020, SFRH\/BD\/108031\/2015, SFRH\/BD\/145600\/2019"],"award-info":[{"award-number":["PTDC\/BBB-BQB\/6071\/2014, UID\/Multi\/00612\/2019, UIDB\/00100\/2020, SFRH\/BD\/108031\/2015, SFRH\/BD\/145600\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The relevance of mannosyldiinositolphosphorylceramide [M(IP)2C] synthesis, the terminal complex sphingolipid class in the yeast Saccharomyces cerevisiae, for the lateral organization of the plasma membrane, and in particular for sphingolipid-enriched gel domains, was investigated by fluorescence spectroscopy and microscopy. We also addressed how changing the complex sphingolipid profile in the plasma membrane could influence the membrane compartments (MC) containing either the arginine\/ H+ symporter Can1p (MCC) or the proton ATPase Pma1p (MCP). To achieve these goals, wild-type (wt) and ipt1\u0394 cells, which are unable to synthesize M(IP)2C accumulating mannosylinositolphosphorylceramide (MIPC), were compared. Living cells, isolated plasma membrane and giant unilamellar vesicles reconstituted from plasma membrane lipids were labelled with various fluorescent membrane probes that report the presence and organization of distinct lipid domains, global order, and dielectric properties. Can1p and Pma1p were tagged with GFP and mRFP, respectively, in both yeast strains, to evaluate their lateral organization using confocal fluorescence intensity and fluorescence lifetime imaging. The results show that IPT1 deletion strongly affects the rigidity of gel domains but not their relative abundance, whereas no significant alterations could be perceived in ergosterol-enriched domains. Moreover, in these cells lacking M(IP)2C, a clear alteration in Pma1p membrane distribution, but no significant changes in Can1p distribution, were observed. Thus, this work reinforces the notion that sphingolipid-enriched domains distinct from ergosterol-enriched regions are present in the S. cerevisiae plasma membrane and suggests that M(IP)2C is important for a proper hydrophobic chain packing of sphingolipids in the gel domains of wt cells. Furthermore, our results strongly support the involvement of sphingolipid domains in the formation and stability of the MCP, possibly being enriched in this compartment.<\/jats:p>","DOI":"10.3390\/biom10060871","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T06:34:16Z","timestamp":1591684456000},"page":"871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Yeast Sphingolipid-Enriched Domains and Membrane Compartments in the Absence of Mannosyldiinositolphosphorylceramide"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1885-3469","authenticated-orcid":false,"given":"Andreia","family":"Bento-Oliveira","sequence":"first","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9632-2300","authenticated-orcid":false,"given":"Filipa C.","family":"Santos","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7509-633X","authenticated-orcid":false,"given":"Joaquim Trigo","family":"Marqu\u00eas","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"given":"Pedro M. R.","family":"Paulo","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural, Instituto Superior T\u00e9cnico, 1049-001 Lisbon, Portugal"}]},{"given":"Thomas","family":"Korte","sequence":"additional","affiliation":[{"name":"Department of Biology, Molecular Biophysics, IRI Life Sciences, Humboldt-Universit\u00e4t zu Berlin, 10115 Berlin, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6716-2026","authenticated-orcid":false,"given":"Andreas","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Department of Biology, Molecular Biophysics, IRI Life Sciences, Humboldt-Universit\u00e4t zu Berlin, 10115 Berlin, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4625-2516","authenticated-orcid":false,"given":"H. Susana","family":"Marinho","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9748-7083","authenticated-orcid":false,"given":"Rodrigo F. M.","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica Estrutural, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/bs.ircmb.2016.02.005","article-title":"New Insight into the Roles of Membrane Microdomains in Physiological Activities of Fungal Cells","volume":"325","author":"Malinsky","year":"2016","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zahumensky, J., and Malinsky, J. (2019). 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