{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T00:58:50Z","timestamp":1774141130325,"version":"3.50.1"},"reference-count":82,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T00:00:00Z","timestamp":1592524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2015-69738-R"],"award-info":[{"award-number":["AGL2015-69738-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["AGL2015-69738-R"],"award-info":[{"award-number":["AGL2015-69738-R"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca","doi-asserted-by":"publisher","award":["19892\/GERM-15"],"award-info":[{"award-number":["19892\/GERM-15"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001862","name":"Svenska Forskningsr\u00e5det Formas","doi-asserted-by":"publisher","award":["2017-00946 and 2019-00288"],"award-info":[{"award-number":["2017-00946 and 2019-00288"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The seminal plasma (SP) modulates the female reproductive immune environment after mating, and microRNAs (miRNAs) could participate in the process. Considering that the boar ejaculate is built by fractions differing in SP-composition, this study evaluated whether exposure of mucosal explants of the sow internal genital tract (uterus, utero-tubal junction and isthmus) to different SP-fractions changed the profile of explant-secreted miRNAs. Mucosal explants retrieved from oestrus sows (n = 3) were in vitro exposed to: Medium 199 (M199, Control) or M199 supplemented (1:40 v\/v) with SP from the sperm-rich fraction (SRF), the post-SRF or the entire recomposed ejaculate, for 16 h. After, the explants were cultured in M199 for 24 h to finally collect the media for miRNA analyses using GeneChip miRNA 4.0 Array (Affymetrix). Fifteen differentially expressed (False Discovery Rate (FDR) &lt; 0.05 and Fold-change \u2265 2) miRNAs (11 down- versus 4 up-regulated) were identified (the most in the media of uterine explants incubated with SP from post-SRF). Bioinformatics analysis identified that predicted target genes of dysregulated miRNAs, mainly miR-34b, miR-205, miR-4776-3p and miR-574-5p, were involved in functions and pathways related to immune response. In conclusion, SP is able to elicit changes in the miRNAs profile secreted by female genital tract, ultimately depending SP-composition.<\/jats:p>","DOI":"10.3390\/biom10060933","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T10:43:58Z","timestamp":1592563438000},"page":"933","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Seminal Plasma Modulates miRNA Expression by Sow Genital Tract Lining Explants"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9873-814X","authenticated-orcid":false,"given":"Isabel","family":"Barranco","sequence":"first","affiliation":[{"name":"Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, E-30100 Murcia, Spain"},{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17003 Girona, Spain"},{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynaecology, Faculty of Medicine and Health Sciences, Link\u00f6ping University, SE-58185 Link\u00f6ping, Sweden"}]},{"given":"Lorena","family":"Padilla","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, E-30100 Murcia, Spain"},{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynaecology, Faculty of Medicine and Health Sciences, Link\u00f6ping University, SE-58185 Link\u00f6ping, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6811-0191","authenticated-orcid":false,"given":"Cristina A.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynaecology, Faculty of Medicine and Health Sciences, Link\u00f6ping University, SE-58185 Link\u00f6ping, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0120-354X","authenticated-orcid":false,"given":"Manuel","family":"Alvarez-Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynaecology, Faculty of Medicine and Health Sciences, Link\u00f6ping University, SE-58185 Link\u00f6ping, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5121-758X","authenticated-orcid":false,"given":"Inmaculada","family":"Parrilla","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, E-30100 Murcia, Spain"}]},{"given":"Xiomara","family":"Lucas","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, E-30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0622-6513","authenticated-orcid":false,"given":"Gra\u00e7a","family":"Ferreira-Dias","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2209-340X","authenticated-orcid":false,"given":"Marc","family":"Yeste","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17003 Girona, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5194-2124","authenticated-orcid":false,"given":"Heriberto","family":"Rodriguez-Martinez","sequence":"additional","affiliation":[{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynaecology, Faculty of Medicine and Health Sciences, Link\u00f6ping University, SE-58185 Link\u00f6ping, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4213-3093","authenticated-orcid":false,"given":"Jordi","family":"Roca","sequence":"additional","affiliation":[{"name":"Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, E-30100 Murcia, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s10815-014-0243-y","article-title":"Seminal fluid and reproduction: Much more than previously thought","volume":"31","author":"Bromfield","year":"2014","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.theriogenology.2019.05.035","article-title":"Potential of seminal plasma to improve the fertility of frozen-thawed boar spermatozoa","volume":"137","author":"Recuero","year":"2019","journal-title":"Theriogenology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5397804","DOI":"10.1155\/2019\/5397804","article-title":"The Potential Role of Seminal Plasma in the Fertilization Outcomes","volume":"2019","author":"Szczykutowicz","year":"2019","journal-title":"Biomed. Res. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s00441-005-1127-3","article-title":"Seminal plasma and male factor signalling in the female reproductive tract","volume":"322","author":"Robertson","year":"2005","journal-title":"Cell Tissue Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1600-0897.2011.01033.x","article-title":"Seminal plasma proteins: What role do they play?","volume":"66","author":"Kvist","year":"2011","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.semcdb.2019.06.008","article-title":"The influence of seminal plasma on offspring development and health","volume":"97","author":"Morgan","year":"2020","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1530\/rep.1.00160","article-title":"Seminal plasma regulates endometrial cytokine expression, leukocyte recruitment and embryo development in the pig","volume":"128","author":"Jasper","year":"2004","journal-title":"Reproduction"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.4049\/jimmunol.1102736","article-title":"Seminal fluid induces leukocyte recruitment and cytokine and chemokine mRNA expression in the human cervix after coitus","volume":"188","author":"Sharkey","year":"2012","journal-title":"J. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/978-3-319-18881-2_6","article-title":"Seminal Fluid Signalling in the Female Reproductive Tract: Implications for Reproductive Success and Offspring Health","volume":"868","author":"Schjenken","year":"2015","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Alvarez-Rodriguez, M., Atikuzzaman, M., Venhoranta, H., Wright, D., and Rodriguez-Martinez, H. (2019). Expression of Immune Regulatory Genes in the Porcine Internal Genital Tract Is Differentially Triggered by Spermatozoa and Seminal Plasma. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20030513"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"465","DOI":"10.3389\/fvets.2019.00465","article-title":"Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs","volume":"6","author":"Martinez","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Seo, J., Jin, D., Choi, C.-H., and Lee, H. (2017). Integration of MicroRNA, mRNA, and Protein Expression Data for the Identification of Cancer-Related MicroRNAs. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0168412"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/nrm1644","article-title":"MicroRNA biogenesis: Coordinated cropping and dicing","volume":"6","author":"Kim","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1146\/annurev-biochem-060308-103103","article-title":"Regulation of mRNA translation and stability by microRNAs","volume":"79","author":"Fabian","year":"2010","journal-title":"Annu. Rev. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/nrg3198","article-title":"MicroRNA profiling: Approaches and considerations","volume":"13","author":"Pritchard","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s12958-017-0309-7","article-title":"Role of microRNAs in embryo implantation","volume":"15","author":"Liang","year":"2017","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1002\/mrd.22823","article-title":"MicroRNA regulation of immune events at conception","volume":"84","author":"Robertson","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Salas-Huetos, A., James, E.R., Aston, K.I., Jenkins, T.G., Carrell, D.T., and Yeste, M. (2019). The Expression of miRNAs in Human Ovaries, Oocytes, Extracellular Vesicles, and Early Embryos: A Systematic Review. Cells, 8.","DOI":"10.3390\/cells8121564"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/andr.12714","article-title":"The role of miRNAs in male human reproduction: A systematic review","volume":"8","author":"James","year":"2020","journal-title":"Andrology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1530\/REP-11-0240","article-title":"The role of micro-RNAs in the female reproductive tract","volume":"143","author":"Nothnick","year":"2012","journal-title":"Reproduction"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1055\/s-0028-1096128","article-title":"MicroRNA signature and regulatory functions in the endometrium during normal and disease states","volume":"26","author":"Pan","year":"2008","journal-title":"Semin. Reprod. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1038\/cmi.2014.45","article-title":"MicroRNAs, immune cells and pregnancy","volume":"11","author":"Bidarimath","year":"2014","journal-title":"Cell. Mol. Immunol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Su, L., Liu, R., Cheng, W., Zhu, M., Li, X., Zhao, S., and Yu, M. (2014). Expression patterns of microRNAs in porcine endometrium and their potential roles in embryo implantation and placentation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087867"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.gene.2017.12.046","article-title":"Sequence analysis of microRNAs during pre-implantation between Meishan and Yorkshire pigs","volume":"646","author":"Li","year":"2018","journal-title":"Gene"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"661","DOI":"10.3389\/fgene.2019.00661","article-title":"Differential microRNA Expression in Porcine Endometrium Involved in Remodeling and Angiogenesis That Contributes to Embryonic Implantation","volume":"10","author":"Hong","year":"2019","journal-title":"Front. Genet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1038\/ni.f.209","article-title":"MicroRNAs: New regulators of immune cell development and function","volume":"9","author":"Baltimore","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/978-1-4419-5632-3_2","article-title":"MicroRNAs: Key components of immune regulation","volume":"780","author":"Gracias","year":"2011","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1111\/aji.12490","article-title":"Mi RNA Regulation of Immune Tolerance in Early Pregnancy","volume":"75","author":"Schjenken","year":"2016","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Schjenken, J.E., Moldenhauer, L.M., Zhang, B., Care, A.S., Groome, H.M., Chan, H.-Y., Hope, C.M., Barry, S.C., and Robertson, S.A. (2020). MicroRNA miR-155 is required for expansion of regulatory T cells to mediate robust pregnancy tolerance in mice. Mucosal Immunol., 1\u201317.","DOI":"10.1038\/s41385-020-0255-0"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/ncb1596","article-title":"Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells","volume":"9","author":"Valadi","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ng, Y.H., Rome, S., Jalabert, A., Forterre, A., Singh, H., Hincks, C.L., and Salamonsen, L.A. (2013). Endometrial exosomes\/microvesicles in the uterine microenvironment: A new paradigm for embryo-endometrial cross talk at implantation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0058502"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e339","DOI":"10.1016\/j.fertnstert.2015.07.1055","article-title":"Secreted endometrial microRNA gene regulators are critical for successful embryo implantation","volume":"104","author":"Parks","year":"2015","journal-title":"Fertil. Steril."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3210","DOI":"10.1242\/dev.124289","article-title":"Hsa-miR-30d, secreted by the human endometrium, is taken up by the pre-implantation embryo and might modify its transcriptome","volume":"142","author":"Vilella","year":"2015","journal-title":"Development"},{"key":"ref_34","first-page":"1","article-title":"The physiological roles of the boar ejaculate","volume":"66","author":"Kvist","year":"2009","journal-title":"Soc. Reprod. Fertil. Suppl."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jri.2009.10.007","article-title":"Spermadhesin PSP-I\/PSP-II heterodimer induces migration of polymorphonuclear neutrophils into the uterine cavity of the sow","volume":"84","author":"Saravia","year":"2010","journal-title":"J. Reprod. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1373\/clinchem.2009.141580","article-title":"Robust microRNA stability in degraded RNA preparations from human tissue and cell samples","volume":"56","author":"Jung","year":"2010","journal-title":"Clin. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1038\/bjc.2012.294","article-title":"Enhanced stability of microRNA expression facilitates classification of FFPE tumour samples exhibiting near total mRNA degradation","volume":"107","author":"Hall","year":"2012","journal-title":"Br. J. Cancer"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1093\/nar\/gkw116","article-title":"Distribution of miRNA expression across human tissues","volume":"44","author":"Ludwig","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"D127","DOI":"10.1093\/nar\/gkz757","article-title":"MiRDB: An online database for prediction of functional microRNA targets","volume":"48","author":"Chen","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Alvarez-Rodriguez, M., Martinez, C., Wright, D., Barranco, I., Roca, J., and Rodriguez-Martinez, H. (2020). The Transcriptome of Pig Spermatozoa, and Its Role in Fertility. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21051572"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jri.2009.08.003","article-title":"Activating T regulatory cells for tolerance in early pregnancy-the contribution of seminal fluid","volume":"83","author":"Robertson","year":"2009","journal-title":"J. Reprod. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1093\/humrep\/14.9.2386","article-title":"Pre-implantation endometrial leukocytes in women with recurrent miscarriage","volume":"14","author":"Quenby","year":"1999","journal-title":"Hum. Reprod."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.fertnstert.2012.08.034","article-title":"Proinflammatory cytokines induced altered expression of cyclooxygenase-2 gene results in unreceptive endometrium in women with idiopathic recurrent spontaneous miscarriage","volume":"99","author":"Banerjee","year":"2013","journal-title":"Fertil. Steril."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1530\/rep.0.1210889","article-title":"Selective sperm binding to pig oviductal epithelium in vitro","volume":"121","author":"Petrunkina","year":"2001","journal-title":"Reproduction"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s10397-009-0520-4","article-title":"Morphology of human endometrial explants and secretion of stromal marker proteins in short- and long-term cultures","volume":"7","author":"Bersinger","year":"2010","journal-title":"Gynecol. Surg."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1111\/j.1600-0897.2012.01106.x","article-title":"Explants of intact endometrium to model bovine innate immunity and inflammation ex vivo","volume":"67","author":"Borges","year":"2012","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s12958-015-0128-7","article-title":"Difference in expression between AQP1 and AQP5 in porcine endometrium and myometrium in response to steroid hormones, oxytocin, arachidonic acid, forskolin and cAMP during the mid-luteal phase of the estrous cycle and luteolysis","volume":"13","author":"Skowronska","year":"2015","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"15072","DOI":"10.1038\/s41598-019-51413-4","article-title":"Species-specific and collection method-dependent differences in endometrial susceptibility to seminal plasma-induced RNA degradation","volume":"9","author":"Sanchez","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1530\/rep.0.1230163","article-title":"Minimum number of spermatozoa required for normal fertility after deep intrauterine insemination in non-sedated sows","volume":"123","author":"Martinez","year":"2002","journal-title":"Reproduction"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0378-4320(02)00230-0","article-title":"Influence of pre-ovulatory insemination and early pregnancy on the infiltration by cells of the immune system in the sow endometrium","volume":"75","author":"Kaeoket","year":"2003","journal-title":"Anim. Reprod. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1093\/biolre\/ioy226","article-title":"Seminal plasma modulates expression of endometrial inflammatory meditators in the bovinedagger","volume":"100","author":"Ibrahim","year":"2019","journal-title":"Biol. Reprod."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1530\/jrf.0.0590453","article-title":"Distribution of small- and medium-sized molecules within the genital tract of artificially inseminated gilts","volume":"59","author":"Einarsson","year":"1980","journal-title":"J. Reprod. Fertil."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Li, P., Chen, Y., Juma, C.A., Yang, C., Huang, J., Zhang, X., and Zeng, Y. (2019). Differential Inhibition of Target Gene Expression by Human microRNAs. Cells, 8.","DOI":"10.3390\/cells8080791"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1126\/science.1149460","article-title":"Switching from repression to activation: microRNAs can up-regulate translation","volume":"318","author":"Vasudevan","year":"2007","journal-title":"Science"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"113","DOI":"10.3109\/19396368.2014.999390","article-title":"Seminal quality in the first fraction of ejaculate","volume":"61","author":"Hebles","year":"2015","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1111\/j.1439-0531.2011.01789.x","article-title":"The association of the presence of seminal plasma and its components with sperm longevity in fractionated stallion ejaculates","volume":"46","author":"Kareskoski","year":"2011","journal-title":"Reprod. Domest. Anim."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jprot.2016.04.026","article-title":"Characterization of the porcine seminal plasma proteome comparing ejaculate portions","volume":"142","author":"Barranco","year":"2016","journal-title":"J. Proteom."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1111\/aji.12432","article-title":"The Seminal Plasma of the Boar is Rich in Cytokines, with Significant Individual and Intra-Ejaculate Variation","volume":"74","author":"Barranco","year":"2015","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1111\/j.1600-0897.1997.tb00257.x","article-title":"Cytokine-leukocyte networks and the establishment of pregnancy","volume":"37","author":"Robertson","year":"1997","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3389\/fimmu.2019.00015","article-title":"Cytokine Targeting by miRNAs in Autoimmune Diseases","volume":"10","author":"Salvi","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1093\/molehr\/gap068","article-title":"MicroRNA expression profiling of eutopic secretory endometrium in women with versus without endometriosis","volume":"15","author":"Burney","year":"2009","journal-title":"Mol. Hum. Reprod."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"E395","DOI":"10.1002\/ijc.27345","article-title":"MicroRNA-34b functions as a potential tumor suppressor in endometrial serous adenocarcinoma","volume":"131","author":"Hiroki","year":"2012","journal-title":"Int. J. Cancer"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s12958-017-0319-5","article-title":"Integration analysis of microRNA and mRNA paired expression profiling identifies deregulated microRNA-transcription factor-gene regulatory networks in ovarian endometriosis","volume":"16","author":"Zhao","year":"2018","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1586\/eci.11.53","article-title":"Immune interactions in endometriosis","volume":"7","author":"Herington","year":"2011","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_65","first-page":"dju257","article-title":"The role of microRNAs in the control of innate immune response in cancer","volume":"106","author":"Mandelboim","year":"2014","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1101\/gad.314617.118","article-title":"Roles of the immune system in cancer: From tumor initiation to metastatic progression","volume":"32","author":"Gonzalez","year":"2018","journal-title":"Genes Dev."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1095\/biolreprod.112.099051","article-title":"Regulatory mechanism for expression of IL1B receptors in the uterine endometrium and effects of IL1B on prostaglandin synthetic enzymes during the implantation period in pigs","volume":"87","author":"Seo","year":"2012","journal-title":"Biol. Reprod."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Salilew-Wondim, D., Ibrahim, S., Gebremedhn, S., Tesfaye, D., Heppelmann, M., Bollwein, H., Pfarrer, C., Tholen, E., Neuhoff, C., and Schellander, K. (2016). Clinical and subclinical endometritis induced alterations in bovine endometrial transcriptome and miRNome profile. BMC Genom., 17.","DOI":"10.1186\/s12864-016-2513-9"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.4049\/jimmunol.1203567","article-title":"MicroRNA-17-92 regulates IL-10 production by regulatory T cells and control of experimental autoimmune encephalomyelitis","volume":"191","author":"Esensten","year":"2013","journal-title":"J. Immunol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/ni1037","article-title":"Regulatory T cells mediate maternal tolerance to the fetus","volume":"5","author":"Aluvihare","year":"2004","journal-title":"Nat. Immunol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/aji.12107","article-title":"Seminal fluid and the generation of regulatory T cells for embryo implantation","volume":"69","author":"Robertson","year":"2013","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"14416","DOI":"10.1038\/srep14416","article-title":"Endometrial gene expression profile of pregnant sows with extreme phenotypes for reproductive efficiency","volume":"5","author":"Cordoba","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Karaayvaz, M., Zhang, C., Liang, S., Shroyer, K.R., and Ju, J. (2012). Prognostic significance of miR-205 in endometrial cancer. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0035158"},{"key":"ref_74","first-page":"2864","article-title":"MiR-200a\/miR-141 and miR-205 upregulation might be associated with hormone receptor status and prognosis in endometrial carcinomas","volume":"8","author":"Dong","year":"2015","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Wilczynski, M., Danielska, J., Dzieniecka, M., Szymanska, B., Wojciechowski, M., and Malinowski, A. (2016). Prognostic and Clinical Significance of miRNA-205 in Endometrioid Endometrial Cancer. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0164687"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1007\/s10753-015-0156-7","article-title":"Reduced Expression of miR-23a Suppresses A20 in TLR-stimulated Macrophages","volume":"38","author":"Peng","year":"2015","journal-title":"Inflammation"},{"key":"ref_77","first-page":"1524","article-title":"MiR23a downregulation modulates the inflammatory response by targeting ATG12mediated autophagy","volume":"18","author":"Si","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.yexcr.2017.03.039","article-title":"MiR-23a-5p modulates mycobacterial survival and autophagy during mycobacterium tuberculosis infection through TLR2\/MyD88\/NF-kappaB pathway by targeting TLR2","volume":"354","author":"Gu","year":"2017","journal-title":"Exp. Cell Res."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s13567-014-0141-4","article-title":"Identification of microRNAs in PCV2 subclinically infected pigs by high throughput sequencing","volume":"46","author":"Perez","year":"2015","journal-title":"Vet. Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1530\/REP-11-0304","article-title":"Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies","volume":"143","author":"Wu","year":"2012","journal-title":"Reproduction"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1186\/s12967-018-1556-x","article-title":"Association of the peripheral blood levels of circulating microRNAs with both recurrent miscarriage and the outcomes of embryo transfer in an in vitro fertilization process","volume":"16","author":"Yang","year":"2018","journal-title":"J. Transl. Med."},{"key":"ref_82","first-page":"3792","article-title":"MicroRNA-23a inhibits endometrial cancer cell development by targeting SIX1","volume":"18","author":"Li","year":"2019","journal-title":"Oncol. Lett."}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/10\/6\/933\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:41:00Z","timestamp":1760175660000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/10\/6\/933"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,19]]},"references-count":82,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["biom10060933"],"URL":"https:\/\/doi.org\/10.3390\/biom10060933","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,19]]}}}