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Within these compounds, phlorotannins are known to possess several bioactivities (e.g., antioxidant, anti-inflammatory, and antimicrobial) with potential to improve wound healing. To obtain phlorotannins enriched extracts from Undaria pinnatifida, a biorefinery was set using low-cost industry-friendly methodologies, such as sequential solid\u2013liquid extraction and liquid\u2013liquid extraction. The obtained extracts were screened for their antioxidant and antimicrobial activity against five common wound pathogens and for their anti-inflammatory potential. The ethanolic wash fraction (wE100) had the highest antioxidant activity (114.61 \u00b1 10.04 mmol\u00b7mg\u22121 extract by Diphenyl-1-picrylhydrazyl (DPPH) and 6.56 \u00b1 1.13 mM eq. Fe II\u00b7mg\u22121 extract by and Ferric Reducing Antioxidant Power (FRAP)), acting efficiently against Gram-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria, and showing a nitric oxide production inhibition over 47% when used at 0.01 \u00b5g\u00b7mL\u22121. NMR and FTIR chemical characterization suggested that phlorotannins are present. Obtained fraction wE100 proved to be a promising candidate for further inclusion as wound healing agents, while the remaining fractions analyzed are potential sources for other biotechnological applications, giving emphasis to a biorefinery and circular economy framework to add value to this seaweed and the industry.<\/jats:p>","DOI":"10.3390\/biom11030461","type":"journal-article","created":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T11:18:38Z","timestamp":1616152718000},"page":"461","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["A Biorefinery Approach to the Biomass of the Seaweed Undaria pinnatifida (Harvey Suringar, 1873): Obtaining Phlorotannins-Enriched Extracts for Wound Healing"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6838-3744","authenticated-orcid":false,"given":"Carolina A. M.","family":"Ferreira","sequence":"first","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2050-641 Peniche, Portugal"},{"name":"CDRSP\u2014Centre for Rapid and Sustainable Product Development, Polit\u00e9cnico de Leiria, 2030-028 Marinha Grande, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4766-3736","authenticated-orcid":false,"given":"Rafael","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2050-641 Peniche, Portugal"}]},{"given":"Carina","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2050-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3228-922X","authenticated-orcid":false,"given":"Adriana P.","family":"Janu\u00e1rio","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2050-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5016-0868","authenticated-orcid":false,"given":"Nuno","family":"Alves","sequence":"additional","affiliation":[{"name":"CDRSP\u2014Centre for Rapid and Sustainable Product Development, Polit\u00e9cnico de Leiria, 2030-028 Marinha Grande, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1306-3396","authenticated-orcid":false,"given":"Sara C.","family":"Novais","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2050-641 Peniche, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-1069","authenticated-orcid":false,"given":"Juliana R.","family":"Dias","sequence":"additional","affiliation":[{"name":"CDRSP\u2014Centre for Rapid and Sustainable Product Development, Polit\u00e9cnico de Leiria, 2030-028 Marinha Grande, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9887-1864","authenticated-orcid":false,"given":"Marco F. L.","family":"Lemos","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre, ESTM, Polit\u00e9cnico de Leiria, 2050-641 Peniche, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.tifs.2019.02.037","article-title":"Bioprospecting of brown seaweeds for biotechnological applications: Phlorotannin actions in inflammation and allergy network","volume":"86","author":"Barbosa","year":"2019","journal-title":"Trends Food Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hu, J., Yang, B., Lin, X., Zhou, X.F., Yang, X.W., and Liu, Y. (2011). Bioactive Metabolites from Seaweeds. Handbook of Marine Macroalgae: Biotechnology and Applied Phycology, John Wiley & Sons, Inc.. [1st ed.]. 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