{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T08:28:04Z","timestamp":1768638484210,"version":"3.49.0"},"reference-count":37,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T00:00:00Z","timestamp":1638230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/5011\/2020"],"award-info":[{"award-number":["UIDB\/5011\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/50011\/2020"],"award-info":[{"award-number":["UIDP\/50011\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Analysis by attenuated total reflection\u2013Fourier transform infrared spectroscopy shows that each coexisting phase in aqueous two-phase systems has a different arrangement of hydrogen bonds. Specific arrangements vary for systems formed by different solutes. The hydrogen bond arrangement is shown to correlate with differences in hydrophobic and electrostatic properties of the different phases of five specific systems, four formed by two polymers and one by a single polymer and salt. The results presented here suggest that the arrangement of hydrogen bonds may be an important factor in phase separation.<\/jats:p>","DOI":"10.3390\/biom11121787","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T20:29:27Z","timestamp":1638304167000},"page":"1787","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Hydrogen Bond Arrangement Is Shown to Differ in Coexisting Phases of Aqueous Two-Phase Systems"],"prefix":"10.3390","volume":"11","author":[{"given":"Pedro P.","family":"Madeira","sequence":"first","affiliation":[{"name":"Department of Chemistry, Centro de Investigacao em Materiais Ceramicos e Compositos, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9670-2634","authenticated-orcid":false,"given":"Amber R.","family":"Titus","sequence":"additional","affiliation":[{"name":"Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH 44114, USA"}]},{"given":"Luisa A.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH 44114, USA"}]},{"given":"Alexander I.","family":"Belgovskiy","sequence":"additional","affiliation":[{"name":"Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH 44114, USA"}]},{"given":"Elizabeth K.","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Physics, Kent State University, Kent, OH 44242, USA"}]},{"suffix":"Jr.","given":"Jay Adin","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Case Western Reserve University, Cleveland, OH 44242, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2474-7630","authenticated-orcid":false,"given":"William V.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Scattering Solutions, Inc., Cleveland, OH 44242, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2721-5995","authenticated-orcid":false,"given":"Anthony E.","family":"Smart","sequence":"additional","affiliation":[{"name":"Scattering Solutions, Inc., Costa Mesa, CA 02138, USA"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4037-5857","authenticated-orcid":false,"given":"Vladimir N.","family":"Uversky","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Byrd Alzheimer\u2019s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33620, USA"}]},{"given":"Boris Y.","family":"Zaslavsky","sequence":"additional","affiliation":[{"name":"Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH 44114, USA"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4574","DOI":"10.1039\/C9CP05810A","article-title":"Interfacial tension and mechanism of liquid-liquid phase separation in aqueous media","volume":"22","author":"Titus","year":"2020","journal-title":"Phys. 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