{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T07:55:19Z","timestamp":1778313319328,"version":"3.51.4"},"reference-count":57,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"National Funds from Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT)","doi-asserted-by":"publisher","award":["UIDB\/Multi\/50016\/2020"],"award-info":[{"award-number":["UIDB\/Multi\/50016\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Chronic wounds are one of the most frequent complications that are associated with diabetes mellitus. The overproduction of reactive oxygen species (ROS) is a key factor in the delayed healing of a chronic wound. In the present work, we develop a novel in situ-forming silk sericin-based hydrogel (SSH) that is produced by a simple methodology using horseradish peroxidase (HRP) crosslinking as an advanced dressing for wound healing. The antioxidant and angiogenic effects were assessed in vitro and in vivo after in situ application using an excisional wound-healing model in a genetically-induced diabetic db\/db mice and though the chick embryo choriollantoic membrane (CAM) assay, respectively. Wounds in diabetic db\/db mice that were treated with SSH closed with reduced granulation tissue, decreased wound edge distance, and wound thickness, when compared to Tegaderm, a dressing that is commonly used in the clinic. The hydrogel also promoted a deposition of collagen fibers with smaller diameter which may have had a boost effect in re-epithelialization. SSH treatment slightly induced two important endogenous antioxidant defenses, superoxide dismutase and catalase. A CAM assay made it possible to observe that SSH led to an increase in the number of newly formed vessels without inducing an inflammatory reaction. The present hydrogel may result in a multi-purpose technology with angiogenic, antioxidant, and anti-inflammatory properties, while advancing efficient and organized tissue regeneration.<\/jats:p>","DOI":"10.3390\/biom12060801","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T10:49:14Z","timestamp":1654771754000},"page":"801","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Exploring Silk Sericin for Diabetic Wounds: An In Situ-Forming Hydrogel to Protect against Oxidative Stress and Improve Tissue Healing and Regeneration"],"prefix":"10.3390","volume":"12","author":[{"given":"Sara","family":"Baptista-Silva","sequence":"first","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4169-005 Porto, Portugal"}]},{"given":"Beatriz G.","family":"Bernardes","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4169-005 Porto, Portugal"},{"name":"I+D Farma Group (GI-1645), Department of Pharmacology, Pharmacy and Pharmaceutical Technology, iMATUS and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4665-1098","authenticated-orcid":false,"given":"Sandra","family":"Borges","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4169-005 Porto, Portugal"}]},{"given":"Ilda","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina, Unidade de Bioqu\u00edmica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9535-368X","authenticated-orcid":false,"given":"Rui","family":"Fernandes","sequence":"additional","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal"}]},{"given":"Susana","family":"Gomes-Guerreiro","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina, Unidade de Bioqu\u00edmica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IPATIMUP, Instituto de Patologia e Imunologia Molecular, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Faculdade de Ci\u00eancias da Nutri\u00e7\u00e3o e Alimenta\u00e7\u00e3o, Universidade do Porto, 4150-180 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8521-2904","authenticated-orcid":false,"given":"Marta Teixeira","family":"Pinto","sequence":"additional","affiliation":[{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IBMC, Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135 Porto, Portugal"}]},{"given":"Manuela","family":"Pintado","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9157-5541","authenticated-orcid":false,"given":"Raquel","family":"Soares","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina, Unidade de Bioqu\u00edmica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9245-4565","authenticated-orcid":false,"given":"Raquel","family":"Costa","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina, Unidade de Bioqu\u00edmica, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"},{"name":"i3S, Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"}]},{"given":"Ana Leite","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina, Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,8]]},"reference":[{"key":"ref_1","unstructured":"WHO (2021, April 22). Rolling Updates on Coronavirus Disease (COVID-19). Available online: https:\/\/www.who.int\/emergencies\/diseases\/novel-coronavirus-2019\/events-as-they-happen."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"108615","DOI":"10.1016\/j.biopha.2019.108615","article-title":"Mechanistic insight into diabetic wounds: Pathogenesis, molecular targets and treatment strategies to pace wound healing","volume":"112","author":"Patel","year":"2019","journal-title":"Biomed. Pharm."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s12325-017-0478-y","article-title":"Chronic Wound Healing: A Review of Current Management and Treatments","volume":"34","author":"Han","year":"2017","journal-title":"Adv. Ther."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6422","DOI":"10.1039\/C8NR10114K","article-title":"Nano-hybrid electrospun non-woven mats made of wool keratin and hydrotalcites as potential bio-active wound dressings","volume":"11","author":"Giuri","year":"2019","journal-title":"Nanoscale"},{"key":"ref_5","unstructured":"Snyder, D., Sullivan, N., Margolis, D., and Schoelles, K. (2020). Skin Substitutes for Treating Chronic Wounds [Internet]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.addr.2018.03.007","article-title":"In situ forming injectable hydrogels for drug delivery and wound repair","volume":"127","author":"Dimatteo","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1146\/annurev-bioeng-060418-052422","article-title":"Hydrogel-Based Strategies to Advance Therapies for Chronic Skin Wounds","volume":"21","author":"Reis","year":"2019","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Alven, S., and Aderibigbe, B.A. (2020). Chitosan and Cellulose-Based Hydrogels for Wound Management. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21249656"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/j.ijbiomac.2019.08.007","article-title":"Biopolymer-based biomaterials for accelerated diabetic wound healing: A critical review","volume":"139","author":"Shah","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1039\/C9BM01207A","article-title":"The fabrication of a highly efficient self-healing hydrogel from natural biopolymers loaded with exosomes for the synergistic promotion of severe wound healing","volume":"8","author":"Wang","year":"2020","journal-title":"Biomater. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.jcis.2019.08.083","article-title":"Mussel-inspired, antibacterial, conductive, antioxidant, injectable composite hydrogel wound dressing to promote the regeneration of infected skin","volume":"556","author":"Liang","year":"2019","journal-title":"J. Colloid Interface Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"111597","DOI":"10.1016\/j.msec.2020.111597","article-title":"Fabrication of antibacterial sericin based hydrogel as an injectable and mouldable wound dressing","volume":"119","author":"Tao","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s00441-019-03015-9","article-title":"Healing potential of injectable Aloe vera hydrogel loaded by adipose-derived stem cell in skin tissue-engineering in a rat burn wound model","volume":"377","author":"Oryan","year":"2019","journal-title":"Cell Tissue Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"37563","DOI":"10.1021\/acsami.7b09395","article-title":"pH and Glucose Dual-Responsive Injectable Hydrogels with Insulin and Fibroblasts as Bioactive Dressings for Diabetic Wound Healing","volume":"9","author":"Zhao","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3315","DOI":"10.1039\/C7TB00571G","article-title":"Injectable bioactive akermanite\/alginate composite hydrogels for in situ skin tissue engineering","volume":"5","author":"Han","year":"2017","journal-title":"J. Mater. Chem. B"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4766","DOI":"10.1021\/acsomega.8b00308","article-title":"Gelatin-Based Hydrogels Blended with Gellan as an Injectable Wound Dressing","volume":"3","author":"Zheng","year":"2018","journal-title":"ACS Omega"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"57782","DOI":"10.1021\/acsami.0c18948","article-title":"Injectable Adhesive Self-Healing Multicross-Linked Double-Network Hydrogel Facilitates Full-Thickness Skin Wound Healing","volume":"12","author":"Yang","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"117447","DOI":"10.1016\/j.lfs.2020.117447","article-title":"Free and hydrogel encapsulated exosome-based therapies in regenerative medicine","volume":"249","author":"Akbari","year":"2020","journal-title":"Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"21015","DOI":"10.1021\/acsomega.0c02580","article-title":"Thermosensitive Injectable Chitosan\/Collagen\/\u03b2-Glycerophosphate Composite Hydrogels for Enhancing Wound Healing by Encapsulating Mesenchymal Stem Cell Spheroids","volume":"5","author":"Yang","year":"2020","journal-title":"ACS Omega"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5096","DOI":"10.1021\/acsbiomaterials.0c00988","article-title":"Mesenchymal Stem Cell Spheroids Embedded in an Injectable Thermosensitive Hydrogel: An In Situ Drug Formation Platform for Accelerated Wound Healing","volume":"6","author":"Nilforoushzadeh","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1021\/acsmacrolett.0c00184","article-title":"Injectable Cucurbit [8]uril-Based Supramolecular Gelatin Hydrogels for Cell Encapsulation","volume":"9","author":"Madl","year":"2020","journal-title":"ACS Macro Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1002\/jbm.b.33400","article-title":"Influence of different surface modification treatments on silk biotextiles for tissue engineering applications","volume":"104","author":"Ribeiro","year":"2015","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e1800465","DOI":"10.1002\/adhm.201800465","article-title":"The Biomedical Use of Silk: Past, Present, Future","volume":"8","author":"Holland","year":"2018","journal-title":"Adv. Healthc. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6486","DOI":"10.1039\/C8CS00187A","article-title":"Silkworm silk-based materials and devices generated using bio-nanotechnology","volume":"47","author":"Huang","year":"2018","journal-title":"Chem. Soc. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1016\/j.progpolymsci.2008.08.002","article-title":"Natural protective glue protein, sericin bioengineered by silkworms: Potential for biomedical and biotechnological applications","volume":"33","author":"Kundu","year":"2008","journal-title":"Prog. Polym. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.3390\/ijms11052200","article-title":"The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production","volume":"11","author":"Aramwit","year":"2010","journal-title":"Int. J. Mol. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Nagai, N., Fukuoka, Y., Ishii, M., Otake, H., Yamamoto, T., Taga, A., Okamoto, N., and Shimomura, Y. (2018). Instillation of Sericin Enhances Corneal Wound Healing through the ERK Pathway in Rat Debrided Corneal Epithelium. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19041123"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Veiga, A., Castro, F., Rocha, F., and Oliveira, A.L. (2020). Recent Advances in Silk Sericin\/Calcium Phosphate Biomaterials. Front. Mater., 7.","DOI":"10.3389\/fmats.2020.00024"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S25","DOI":"10.12968\/jowc.2020.29.Sup4.S25","article-title":"Antibiofilm activity and cytotoxicity of silk sericin against Streptococcus mutans bacteria in biofilm: An in vitro study","volume":"29","author":"Aramwit","year":"2020","journal-title":"J. Wound Care"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s13346-016-0322-y","article-title":"Wound healing applications of sericin\/chitosan-capped silver nanoparticles incorporated hydrogel","volume":"7","author":"Verma","year":"2016","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.biomaterials.2018.02.016","article-title":"Photo-crosslinkable, injectable sericin hydrogel as 3D biomimetic extracellular matrix for minimally invasive repairing cartilage","volume":"163","author":"Qi","year":"2018","journal-title":"Biomaterials"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Jo, Y.-Y., Kweon, H., and Oh, J.-H. (2020). Sericin for Tissue Engineering. Appl. Sci., 10.","DOI":"10.3390\/app10238457"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1021\/acsbiomaterials.0c01745","article-title":"In Situ Forming Silk Sericin-Based Hydrogel: A Novel Wound Healing Biomaterial","volume":"7","author":"Borges","year":"2021","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3539","DOI":"10.1039\/D0FO00188K","article-title":"Enzymatic hydrolysis of insect Alphitobius diaperinus towards the development of bioactive peptide hydrolysates","volume":"11","author":"Sousa","year":"2020","journal-title":"Food Funct."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"109847","DOI":"10.1016\/j.msec.2019.109847","article-title":"Conjugation of the T1 sequence from CCN1 to fibrin hydrogels for therapeutic vascularization","volume":"104","author":"Loureiro","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Pinto, A., Pinto, M.L., Velho, S., Pinto, M.T., Cardoso, A.P., Figueira, R., Monteiro, A., Marques, M., Seruca, R., and Barbosa, M.A. (2016). Intricate Macrophage-Colorectal Cancer Cell Communication in Response to Radiation. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0160891"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/j.peptides.2011.06.005","article-title":"Wound healing activity of the human antimicrobial peptide LL37","volume":"32","author":"Ramos","year":"2011","journal-title":"Peptides"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Costa, R., Costa, L., Rodrigues, I., Meireles, C., Soares, R., Tamagnini, P., and Mota, R. (2021). Biocompatibility of the Biopolymer Cyanoflan for Applications in Skin Wound Healing. Mar. Drugs, 19.","DOI":"10.3390\/md19030147"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Costa, R., Azevedo, D., Barata, P., Soares, R., Guido, L., and Carvalho, D. (2021). Antiangiogenic and Antioxidant In Vitro Properties of Hydroethanolic Extract from a\u00e7a\u00ed (Euterpe oleracea) Dietary Powder Supplement. Molecules, 26.","DOI":"10.3390\/molecules26072011"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1002\/masy.201450312","article-title":"Mechanical and Thermal Properties of Sericin\/PVA\/Bentonite Scaffold: Comparison between Uncrosslinked and Crosslinked","volume":"337","author":"Likitamporn","year":"2014","journal-title":"Macromol. Symp."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"364","DOI":"10.3892\/br.2014.244","article-title":"Antioxidant activities of two sericin proteins extracted from cocoon of silkworm (Bombyx mori) measured by DPPH, chemiluminescence, ORAC and ESR methods","volume":"2","author":"Takechi","year":"2014","journal-title":"Biomed. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ustuner, O., Anlas, C., Bakirel, T., Ustun-Alkan, F., Sigirci, B.D., Ak, S., Akpulat, H.A., Donmez, C., and Koca-Caliskan, U. (2019). In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas. Molecules, 24.","DOI":"10.3390\/molecules24183353"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2016\/8175701","article-title":"Silkworm Sericin: Properties and Biomedical Applications","volume":"2016","author":"Kunz","year":"2016","journal-title":"BioMed Res. Int."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.carbpol.2013.01.023","article-title":"Influence of sericin\/TiO2 nanocomposite on cotton fabric: Part 1. Enhanced antibacterial effect","volume":"94","author":"Doakhan","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1080\/00405000.2011.586151","article-title":"Extraction and application of natural silk protein sericin from Bombyx mori as antimicrobial finish for cotton fabrics","volume":"103","author":"Rajendran","year":"2012","journal-title":"J. Text. Inst."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Manesa, K.C., Kebede, T.G., Dube, S., and Nindi, M.M. (2020). Profiling of Silk Sericin from Cocoons of Three Southern African Wild Silk Moths with a Focus on Their Antimicrobial and Antioxidant Properties. Materials, 13.","DOI":"10.3390\/ma13245706"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4663","DOI":"10.1128\/AEM.00643-16","article-title":"Shape Changes and Interaction Mechanism of Escherichia coli Cells Treated with Sericin and Use of a Sericin-Based Hydrogel for Wound Healing","volume":"82","author":"Xue","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1002\/bip.22323","article-title":"Facts and myths of antibacterial properties of silk","volume":"101","author":"Kaur","year":"2013","journal-title":"Biopolymers"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1089\/wound.2014.0556","article-title":"Clinical and Antibiofilm Efficacy of Antimicrobial Hydrogels","volume":"4","author":"Finnegan","year":"2015","journal-title":"Adv. Wound Care"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"104026","DOI":"10.1016\/j.mvr.2020.104026","article-title":"The use of the chick embryo CAM assay in the study of angiogenic activiy of biomaterials","volume":"131","author":"Ribatti","year":"2020","journal-title":"Microvasc. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.mod.2016.05.003","article-title":"The chick embryo chorioallantoic membrane (CAM). A multifaceted experimental model","volume":"141","author":"Ribatti","year":"2016","journal-title":"Mech. Dev."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Johnson, B.Z., Stevenson, A.W., Pr\u00eale, C.M., Fear, M.W., and Wood, F.M. (2020). The Role of IL-6 in Skin Fibrosis and Cutaneous Wound Healing. Biomedicines, 8.","DOI":"10.3390\/biomedicines8050101"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3861","DOI":"10.1007\/s00018-016-2268-0","article-title":"Transition from inflammation to proliferation: A critical step during wound healing","volume":"73","author":"Li","year":"2016","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S0174-173X(85)80002-9","article-title":"Collagen Fiber Formation in Repair Tissue: Development of Strength and Toughness","volume":"5","author":"Doillon","year":"1985","journal-title":"Collagen Relat. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/j.actbio.2013.09.040","article-title":"Chitosan-based dressings loaded with neurotensin\u2014An efficient strategy to improve early diabetic wound healing","volume":"10","author":"Moura","year":"2014","journal-title":"Acta Biomater."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Tottoli, E.M., Dorati, R., Genta, I., Chiesa, E., Pisani, S., and Conti, B. (2020). Skin Wound Healing Process and New Emerging Technologies for Skin Wound Care and Regeneration. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12080735"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Cano Sanchez, M., Lancel, S., Boulanger, E., and Neviere, R. (2018). Targeting Oxidative Stress and Mitochondrial Dysfunction in the Treatment of Impaired Wound Healing: A Systematic Review. Antioxidants, 7.","DOI":"10.3390\/antiox7080098"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/12\/6\/801\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:26:02Z","timestamp":1760138762000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/12\/6\/801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,8]]},"references-count":57,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["biom12060801"],"URL":"https:\/\/doi.org\/10.3390\/biom12060801","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,8]]}}}