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The same complex can be reconstituted from recombinantly produced components in vitro. Assembly of \u03b16 and \u03b17 with Pba3-Pba4 depends on the presence of the \u03b15 subunit, the binding of which apparently initiates the formation of this intermediate. Our data suggest the following order of events: first, Pba3-Pba4 binds \u03b15, then \u03b16 is incorporated, and at the end \u03b17. In the absence of the chaperones Pba1-Pba2 or Ump1, alternative Pba4-containing complexes are detected, the formation of which depends on the Blm10\/PA200 protein. Overexpression of Pba1-Pba2 abolishes the formation of these complexes containing Pba4 and Blm10, suggesting that Blm10 may replace Pba1-Pba2 as an alternative assembly factor.<\/jats:p>","DOI":"10.3390\/biom15050683","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T04:06:21Z","timestamp":1746677181000},"page":"683","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The \u03b15-\u03b16-\u03b17-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6385-9117","authenticated-orcid":false,"given":"Ana C.","family":"Matias","sequence":"first","affiliation":[{"name":"Center of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, Germany"},{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, 8000-117 Faro, Portugal"}]},{"given":"Margarida N.","family":"Tiago","sequence":"additional","affiliation":[{"name":"Center of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, Germany"},{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, 8000-117 Faro, Portugal"}]},{"given":"Jessica","family":"Zimmermann","sequence":"additional","affiliation":[{"name":"Center of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5756-6780","authenticated-orcid":false,"given":"R. J\u00fcrgen","family":"Dohmen","sequence":"additional","affiliation":[{"name":"Center of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5007-3894","authenticated-orcid":false,"given":"Paula C.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Center of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, Germany"},{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade do Algarve, 8000-117 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1021\/cr8004857","article-title":"Catalytic mechanism and assembly of the proteasome","volume":"109","author":"Marques","year":"2009","journal-title":"Chem. 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