{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:31:17Z","timestamp":1776087077921,"version":"3.50.1"},"reference-count":86,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T00:00:00Z","timestamp":1754265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["2020.07845.BD"],"award-info":[{"award-number":["2020.07845.BD"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["2022.09707.BD"],"award-info":[{"award-number":["2022.09707.BD"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["U-IDB\/04423\/2020"],"award-info":[{"award-number":["U-IDB\/04423\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["101086234"],"award-info":[{"award-number":["101086234"]}]},{"name":"European Structural and Investment Funds (ESIF)","award":["2020.07845.BD"],"award-info":[{"award-number":["2020.07845.BD"]}]},{"name":"European Structural and Investment Funds (ESIF)","award":["2022.09707.BD"],"award-info":[{"award-number":["2022.09707.BD"]}]},{"name":"European Structural and Investment Funds (ESIF)","award":["U-IDB\/04423\/2020"],"award-info":[{"award-number":["U-IDB\/04423\/2020"]}]},{"name":"European Structural and Investment Funds (ESIF)","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"European Structural and Investment Funds (ESIF)","award":["101086234"],"award-info":[{"award-number":["101086234"]}]},{"name":"European Union","award":["2020.07845.BD"],"award-info":[{"award-number":["2020.07845.BD"]}]},{"name":"European Union","award":["2022.09707.BD"],"award-info":[{"award-number":["2022.09707.BD"]}]},{"name":"European Union","award":["U-IDB\/04423\/2020"],"award-info":[{"award-number":["U-IDB\/04423\/2020"]}]},{"name":"European Union","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"European Union","award":["101086234"],"award-info":[{"award-number":["101086234"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>The white-spotted jellyfish, Phyllorhiza punctata, is an invasive species with significant ecological and economic relevance spreading across various regions. While its ecological impact is well-documented, its molecular and biochemical characteristics remain poorly understood. In this study, we integrate proteomic data generated by LC-MS\/MS with publicly available transcriptomic information to characterize P. punctata, analyzing differential protein expression across three distinct tissues: oral arms, mantle, and gonads. A total of 2764 proteins and 25,045 peptides were identified, including several venom components such as jellyfish toxins (JFTs) and phospholipase A2 (PLA2), which were further investigated and compared to toxins from other species. Enrichment analyses revealed clear tissue-specific functions. Additionally, deep learning and machine learning tools identified 274 promising AMP candidates, including the \u03b1-helical, \u03b2-sheet, and \u03b1\u03b2-motif peptides. This dataset provides new insights into the protein composition of P. punctata and highlights strong AMP candidates for further characterization, underscoring the biotechnological potential of underexplored cnidarian species.<\/jats:p>","DOI":"10.3390\/biom15081121","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T09:41:17Z","timestamp":1754300477000},"page":"1121","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Unveiling the Bioactive Potential of the Invasive Jellyfish Phyllorhiza punctata Through Integrative Transcriptomic and Proteomic Analyses"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0668-7264","authenticated-orcid":false,"given":"Tom\u00e1s","family":"Rodrigues","sequence":"first","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5277-3940","authenticated-orcid":false,"given":"Ricardo Alexandre","family":"Barroso","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4621-885X","authenticated-orcid":false,"given":"Alexandre","family":"Campos","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9874-933X","authenticated-orcid":false,"given":"Daniela","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Zoology and Physical Anthropology, Faculty of Biology, University of Murcia, Campus of International Excellence, Campus Mare Nostrum, 30100 Murcia, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1018-5446","authenticated-orcid":false,"given":"Francisco A.","family":"Guardiola","sequence":"additional","affiliation":[{"name":"Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia, 30100 Murcia, Spain"}]},{"given":"Maria V.","family":"Turkina","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences, Faculty of Medicine and Clinical Sciences, Link\u00f6ping University, 581 83 Link\u00f6ping, Sweden"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1328-1732","authenticated-orcid":false,"given":"Agostinho","family":"Antunes","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Porto, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.5194\/bg-18-1291-2021","article-title":"Role of Jellyfish in the Plankton Ecosystem Revealed Using a Global Ocean Biogeochemical Model","volume":"18","author":"Wright","year":"2021","journal-title":"Biogeosciences"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3389\/fmars.2018.00257","article-title":"Boom and Bust: Life History, Environmental Noise, and the (un)Predictability of Jellyfish Blooms","volume":"5","author":"Mariani","year":"2018","journal-title":"Front. Mar. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1016\/j.toxicon.2009.02.024","article-title":"Structural Diversity, Systematics, and Evolution of Cnidae","volume":"54","author":"Fautin","year":"2009","journal-title":"Toxicon"},{"key":"ref_4","first-page":"242","article-title":"Jellyfish Impacts on Marine Aquaculture and Fisheries","volume":"29","author":"Milisenda","year":"2020","journal-title":"Rev. Fish. Sci. Aquac."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3354\/meps12536","article-title":"Jellyfish Blooms: Advances and Challenges","volume":"591","author":"Fuentes","year":"2018","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/1054-3139(95)80072-7","article-title":"Medusae, Siphonophores, and Ctenophores as Planktivorous Predators in Changing Global Ecosystems","volume":"52","author":"Mills","year":"1995","journal-title":"ICES J. Mar. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Heaslip, S.G., Iverson, S.J., Bowen, W.D., and James, M.C. (2012). Jellyfish Support High Energy Intake of Leatherback Sea Turtles (Dermochelys coriacea): Video Evidence from Animal-Borne Cameras. PLoS ONE, 7.","DOI":"10.1371\/annotation\/4722cac5-8305-4b03-b805-a59ced1eea49"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1017\/S0025315405011458","article-title":"Predation on Pelagic Coelenterates: A Review","volume":"85","author":"Arai","year":"2005","journal-title":"J. Mar. Biol. Assoc. United Kingd."},{"key":"ref_9","first-page":"105","article-title":"Ecological and Societal Benefits of Jellyfish","volume":"Volume 9789400770","author":"Pitt","year":"2014","journal-title":"Jellyfish Blooms"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1016\/j.tree.2018.09.001","article-title":"A Paradigm Shift in the Trophic Importance of Jellyfish?","volume":"33","author":"Hays","year":"2018","journal-title":"Trends Ecol. Evol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Raposo, A., Alasqah, I., Alfheeaid, H.A., Alsharari, Z.D., Alturki, H.A., and Raheem, D. (2022). Jellyfish as Food: A Narrative Review. Foods, 11.","DOI":"10.3390\/foods11182773"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/raq.12597","article-title":"An Overview of Jellyfish Aquaculture: For Food, Feed, Pharma and Fun","volume":"14","author":"Duarte","year":"2021","journal-title":"Rev. Aquac."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gershwin, L. (2016). Jellyfish: A Natural History, University of Chicago Press.","DOI":"10.7208\/chicago\/9780226287706.001.0001"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Merquiol, L., Romano, G., Ianora, A., and D\u2019Ambra, I. (2019). Biotechnological Applications of Scyphomedusae. Mar. Drugs, 17.","DOI":"10.3390\/md17110604"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s41208-022-00409-5","article-title":"First Record of Mastigias sidereus Chun, 1896 and Phyllorhiza punctata von Lendenfeld, 1884 (Cnidaria: Scyphozoa) from Sri Lanka","volume":"38","author":"Karunarathne","year":"2022","journal-title":"Thalass. An Int. J. Mar. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Morej\u00f3n-Arrojo, R.D., Sellares-Blasco, R., Guendulain-Garc\u00eda, S.D., del Cid, M., and Rodr\u00edguez-Viera, L. (2024). First Occurrence of the Invasive Jellyfish Phyllorhiza punctata in the Dominican Republic: Seasonal Trend and Invasion in the Atlantic Ocean, University of Miami\u2014Rosenstiel School of Marine, Atmospheric & Earth Science.","DOI":"10.5343\/bms.2024.0061"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1023\/A:1024046707234","article-title":"Ecological and Economic Implications of a Tropical Jellyfish Invader in the Gulf of Mexico","volume":"5","author":"Graham","year":"2003","journal-title":"Biol. Invasions"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"709","DOI":"10.12681\/mms.38075","article-title":"First Report of Phyllorhiza punctata von Lendenfeld, 1884 (Cnidaria: Scyphozoa, Mastigiidae) in the Balearic Islands (Western Mediterranean Sea)","volume":"25","author":"Catanese","year":"2024","journal-title":"Mediterr. Mar. Sci."},{"key":"ref_19","first-page":"349","article-title":"First Record of Phyllorhiza punctata (Cnidaria: Rhizostomae: Mastigiidae) in the Northeast Atlantic Ocean","volume":"61","author":"Prieto","year":"2020","journal-title":"Cah. Biol. Mar."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/s00227-021-03969-x","article-title":"Euryhalinity and Thermal Tolerance of Phyllorhiza punctata (Scyphozoa) Scyphostomae: Life History and Physiological Trade-Offs","volume":"168","author":"Rato","year":"2021","journal-title":"Mar. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"108699","DOI":"10.1016\/j.ecss.2024.108699","article-title":"Mediterranean Biogeography, Colonization, Expansion, Phenology, and Life Cycle of the Invasive Jellyfish Phyllorhiza punctata von Lendenfeld, 1884","volume":"299","author":"Marcos","year":"2024","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.chemphyslip.2006.11.001","article-title":"Glyco- and Sphingophosphonolipids from the Medusa Phyllorhiza punctata: NMR and ESI-MS\/MS Fingerprints","volume":"145","author":"Iacomini","year":"2007","journal-title":"Chem. Phys. Lipids"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1002\/jat.1620","article-title":"The Extract of the Jellyfish Phyllorhiza punctata Promotes Neurotoxic Effects","volume":"31","author":"Carneiro","year":"2011","journal-title":"J. Appl. Toxicol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"D377","DOI":"10.1093\/nar\/gkac933","article-title":"CAMPR4: A Database of Natural and Synthetic Antimicrobial Peptides","volume":"51","author":"Gawde","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e00299-21","DOI":"10.1128\/mSystems.00299-21","article-title":"AI4AMP: An Antimicrobial Peptide Predictor Using Physicochemical Property-Based Encoding Method and Deep Learning","volume":"6","author":"Lin","year":"2021","journal-title":"mSystems"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1093\/bioinformatics\/bty179","article-title":"Deep Learning Improves Antimicrobial Peptide Recognition","volume":"34","author":"Veltri","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7974","DOI":"10.1038\/s41467-023-43010-x","article-title":"Accurate de Novo Peptide Sequencing Using Fully Convolutional Neural Networks","volume":"14","author":"Liu","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3172","DOI":"10.1021\/acs.jcim.1c00175","article-title":"Computational Methods and Tools in Antimicrobial Peptide Research","volume":"61","author":"Aronica","year":"2021","journal-title":"J. Chem. Inf. Model."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"baac011","DOI":"10.1093\/database\/baac011","article-title":"A Review on Antimicrobial Peptides Databases and the Computational Tools","volume":"2022","author":"Ramazi","year":"2022","journal-title":"Database"},{"key":"ref_30","unstructured":"Association of Zoos and Aquariums (2013). AZA Aquatic Invertebrate TAG Jellyfish Care Manual, Association of Zoos and Aquariums."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal Sample Preparation Method for Proteome Analysis","volume":"6","author":"Zougman","year":"2009","journal-title":"Nat. Methods"},{"key":"ref_32","unstructured":"Andrews, S. (2025, January 24). FastQC: A Quality Control Tool for High Throughput Sequence Data. Available online: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-Length Transcriptome Assembly from RNA-Seq Data without a Reference Genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2013.084","article-title":"De Novo Transcript Sequence Reconstruction from RNA-Seq Using the Trinity Platform for Reference Generation and Analysis","volume":"8","author":"Haas","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and Sensitive Protein Alignment Using DIAMOND","volume":"12","author":"Buchfink","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Barroso, R.A., Rodrigues, T., Campos, A., Almeida, D., Guardiola, F.A., Turkina, M.V., and Antunes, A. (2025). Proteomic Diversity of the Sea Anemone Actinia fragacea: Comparative Analysis of Nematocyst Venom, Mucus, and Tissue-Specific Profiles. Mar. Drugs, 23.","DOI":"10.3390\/md23020079"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4646","DOI":"10.1021\/ac0341261","article-title":"A Statistical Model for Identifying Proteins by Tandem Mass Spectrometry","volume":"75","author":"Nesvizhskii","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"D344","DOI":"10.1093\/nar\/gkaa977","article-title":"The InterPro Protein Families and Domains Database: 20 Years On","volume":"49","author":"Blum","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1093\/bioinformatics\/btr088","article-title":"ProtTest 3: Fast Selection of Best-Fit Models of Protein Evolution","volume":"27","author":"Darriba","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Walker, J.M. (2005). Protein Identification and Analysis Tools on the ExPASy Server. The Proteomics Protocols Handbook, Humana Press.","DOI":"10.1385\/1592598900"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"bbab242","DOI":"10.1093\/bib\/bbab242","article-title":"AniAMPpred: Artificial Intelligence Guided Discovery of Novel Antimicrobial Peptides in Animal Kingdom","volume":"22","author":"Sharma","year":"2021","journal-title":"Brief. Bioinform."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Han, J., Kong, T., and Liu, J. (2024). PepNet: An Interpretable Neural Network for Anti-Inflammatory and Antimicrobial Peptides Prediction Using a Pre-Trained Protein Language Model. Commun. Biol., 7.","DOI":"10.1038\/s42003-024-06911-1"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Chen, Z., Ji, C., Xu, W., Gao, J., Huang, J., Xu, H., Qian, G., and Huang, J. (2025). UniAMP: Enhancing AMP Prediction Using Deep Neural Networks with Inferred Information of Peptides. BMC Bioinform., 26.","DOI":"10.1186\/s12859-025-06033-3"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Rodrigues, T., Guardiola, F.A., Almeida, D., and Antunes, A. (2025). Aquatic Invertebrate Antimicrobial Peptides in the Fight Against Aquaculture Pathogens. Microorganisms, 13.","DOI":"10.3390\/microorganisms13010156"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/s41592-022-01488-1","article-title":"ColabFold: Making Protein Folding Accessible to All","volume":"19","author":"Mirdita","year":"2022","journal-title":"Nat. Methods"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly Accurate Protein Structure Prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_48","first-page":"432","article-title":"A Refined PH-Dependent Coarse-Grained Model for Peptide Structure Prediction in Aqueous Solution","volume":"3","author":"Derreumaux","year":"2023","journal-title":"Front. Bioinform."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e4792","DOI":"10.1002\/pro.4792","article-title":"UCSF ChimeraX: Tools for Structure Building and Analysis","volume":"32","author":"Meng","year":"2023","journal-title":"Protein Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"jeb191148","DOI":"10.1242\/jeb.191148","article-title":"Jet-Paddling Jellies: Swimming Performance in the Rhizostomeae Jellyfish Catostylus mosaicus","volume":"221","author":"Neil","year":"2018","journal-title":"J. Exp. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1146\/annurev-biophys-042910-155359","article-title":"Actin Structure and Function","volume":"40","author":"Dominguez","year":"2011","journal-title":"Annu. Rev. Biophys."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"7776","DOI":"10.1038\/s41598-018-24817-x","article-title":"Smooth Muscle-like Ca2+-Regulation of Actin-Myosin Interaction in Adult Jellyfish Striated Muscle","volume":"8","author":"Tanaka","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1515\/bmc-2019-0001","article-title":"ATP Synthase: Structure, Function and Inhibition","volume":"10","author":"Neupane","year":"2019","journal-title":"Biomol. Concepts"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e161","DOI":"10.1002\/mco2.161","article-title":"Heat Shock Proteins: Biological Functions, Pathological Roles, and Therapeutic Opportunities","volume":"3","author":"Hu","year":"2022","journal-title":"MedComm"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1146\/annurev-cellbio-101011-155716","article-title":"Diversity of Clathrin Function: New Tricks for an Old Protein","volume":"28","author":"Brodsky","year":"2012","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"194","DOI":"10.3109\/03602532.2011.560157","article-title":"Sigma-Class Glutathione Transferases","volume":"43","author":"Flanagan","year":"2011","journal-title":"Drug Metab. Rev."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1111\/j.1939-1676.1999.tb02185.x","article-title":"An Overview of Proteinase Inhibitors","volume":"13","author":"Hibbetts","year":"1999","journal-title":"J. Vet. Intern. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"104206","DOI":"10.1016\/j.tifs.2023.104206","article-title":"The Emergence of Jellyfish Collagen: A Comprehensive Review on Research Progress, Industrial Applications, and Future Opportunities","volume":"141","author":"Chiarelli","year":"2023","journal-title":"Trends Food Sci. Technol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1021\/acsbiomaterials.7b00979","article-title":"Type I Collagen from Jellyfish Catostylus mosaicus for Biomaterial Applications","volume":"4","author":"Rastian","year":"2018","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1080\/07388550701334212","article-title":"Integrative Analysis of Transcriptomic and Proteomic Data: Challenges, Solutions and Applications","volume":"27","author":"Nie","year":"2007","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1186\/1471-2164-15-884","article-title":"A Comparison of Gene Transcription Profiles of Domesticated and Wild Atlantic Salmon (Salmo salar L.) at Early Life Stages, Reared under Controlled Conditions","volume":"15","author":"Bicskei","year":"2014","journal-title":"BMC Genom."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3541","DOI":"10.1111\/mec.16947","article-title":"Captivity Induces a Sweeping and Sustained Genomic Response in a Starfish","volume":"32","author":"Morin","year":"2023","journal-title":"Mol. Ecol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nrg3185","article-title":"Insights into the Regulation of Protein Abundance from Proteomic and Transcriptomic Analyses","volume":"13","author":"Vogel","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/7651_2015_242","article-title":"Integrated Analysis of Transcriptomic and Proteomic Datasets Reveals Information on Protein Expressivity and Factors Affecting Translational Efficiency","volume":"1375","author":"Wang","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Shen, Y., Wang, Z., Jia, Y., and Liu, X. (2025). Integrated Genomic and Transcriptomic Analysis Reveals a Transcription Factor Gene Set Facilitating Gonadal Differentiation in the Pacific Oyster Crassostrea gigas. Genes, 16.","DOI":"10.3390\/genes16050513"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.cell.2009.02.009","article-title":"The Spliceosome: Design Principles of a Dynamic RNP Machine","volume":"136","author":"Wahl","year":"2009","journal-title":"Cell"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2255","DOI":"10.1095\/biolreprod.102.012450","article-title":"Sex Steroids in Scleractinian Coral, Euphyllia ancora: Implication in Mass Spawning1","volume":"68","author":"Twan","year":"2003","journal-title":"Biol. Reprod."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"529","DOI":"10.2174\/1568026013394831","article-title":"Toxic Metals and Oxidative Stress Part I: Mechanisms Involved in Metal Induced Oxidative Damage","volume":"1","author":"Ercal","year":"2001","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"e57578","DOI":"10.7554\/eLife.57578","article-title":"A Molecular Filter for the Cnidarian Stinging Response","volume":"9","author":"Weir","year":"2020","journal-title":"Elife"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.etap.2007.04.002","article-title":"Different Effects of Pb2+ and Cu2+ on Immune and Antioxidant Enzyme Activities in the Mantle of Pinctada fucata","volume":"24","author":"Jing","year":"2007","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/s42003-020-0777-8","article-title":"Cassiosomes Are Stinging-Cell Structures in the Mucus of the Upside-down Jellyfish Cassiopea xamachana","volume":"3","author":"Ames","year":"2020","journal-title":"Commun. Biol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"4798","DOI":"10.1074\/jbc.M113.534149","article-title":"Chironex fleckeri (Box Jellyfish) Venom Proteins: Expansion of a Cnidarian Toxin Family That Elicits Variable Cytolytic and Cardiovascular Effects","volume":"289","author":"Brinkman","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Ponce, D., Brinkman, D.L., Potriquet, J., and Mulvenna, J. (2016). Tentacle Transcriptome and Venom Proteome of the Pacific Sea Nettle, Chrysaora fuscescens (Cnidaria: Scyphozoa). Toxins, 8.","DOI":"10.3390\/toxins8040102"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Klompen, A.M.L.L., Kayal, E., Collins, A.G., and Cartwright, P. (2021). Phylogenetic and Selection Analysis of an Expanded Family of Putatively Pore-Forming Jellyfish Toxins (Cnidaria: Medusozoa). Genome Biol. Evol., 13.","DOI":"10.1093\/gbe\/evab081"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Klompen, A.M.L., Travert, M.K., and Cartwright, P. (2023). Localization of Multiple Jellyfish Toxins Shows Specificity for Functionally Distinct Polyps and Nematocyst Types in a Colonial Hydrozoan. Toxins, 15.","DOI":"10.3390\/toxins15020149"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Klompen, A.M.L., Macrander, J., Reitzel, A.M., and Stampar, S.N. (2020). Transcriptomic Analysis of Four Cerianthid (Cnidaria, Ceriantharia) Venoms. Mar. Drugs, 18.","DOI":"10.1101\/2020.06.18.159541"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1074\/mcp.RA118.000972","article-title":"Conodipine-P1-3, the First Phospholipases A2 Characterized from Injected Cone Snail Venom","volume":"18","author":"Clark","year":"2019","journal-title":"Mol. Cell. Proteom."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1146\/annurev.genom.9.081307.164356","article-title":"The Toxicogenomic Multiverse: Convergent Recruitment of Proteins into Animal Venoms","volume":"10","author":"Fry","year":"2009","journal-title":"Annu. Rev. Genom. Hum. Genet."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.cbpc.2004.09.006","article-title":"Phospholipase A2 in Cnidaria","volume":"139","author":"Nevalainen","year":"2004","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0005-2736(99)00198-4","article-title":"Diversity of Antimicrobial Peptides and Their Mechanisms of Action","volume":"1462","author":"Epand","year":"1999","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.bbrc.2006.07.078","article-title":"Aurelin, a Novel Antimicrobial Peptide from Jellyfish Aurelia aurita with Structural Features of Defensins and Channel-Blocking Toxins","volume":"348","author":"Ovchinnikova","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1128\/AAC.00826-09","article-title":"Activity of the Novel Peptide Arminin against Multiresistant Human Pathogens Shows the Considerable Potential of Phylogenetically Ancient Organisms as Drug Sources","volume":"53","author":"Augustin","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"22688","DOI":"10.1074\/jbc.M110.216358","article-title":"Innate Immune Responses of a Scleractinian Coral to Vibriosis","volume":"286","author":"Meistertzheim","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"103866","DOI":"10.1016\/j.dci.2020.103866","article-title":"AmAMP1 from Acropora millepora and Damicornin Define a Family of Coral-Specific Antimicrobial Peptides Related to the Shk Toxins of Sea Anemones","volume":"114","author":"Mason","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1021\/bi00431a033","article-title":"Determination of the Three-Dimensional Solution Structure of the Antihypertensive and Antiviral Protein BDS-I from the Sea Anemone Anemonia sulcata: A Study Using Nuclear Magnetic Resonance and Hybrid Distance Geometry\u2013Dynamical Simulated Annealing","volume":"28","author":"Driscoll","year":"1989","journal-title":"Biochemistry"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.2174\/0929866511320100010","article-title":"Identification of a Novel Antimicrobial Peptide from Brazilian Coast Coral Phyllogorgia dilatata","volume":"20","author":"Lima","year":"2013","journal-title":"Protein Pept. 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