{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T02:03:27Z","timestamp":1770689007704,"version":"3.49.0"},"reference-count":70,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T00:00:00Z","timestamp":1438646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, Portugal","award":["ENMed\/0001\/2013"],"award-info":[{"award-number":["ENMed\/0001\/2013"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, Portugal","award":["JPND\/0003\/2011"],"award-info":[{"award-number":["JPND\/0003\/2011"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, Portugal","award":["Pest-OE\/EQB\/LA0004\/2011"],"award-info":[{"award-number":["Pest-OE\/EQB\/LA0004\/2011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Cells release vesicles to the extracellular environment with characteristic nucleic acid, protein, lipid, and glycan composition. Here we have isolated and characterized extracellular vesicles (EVs) and total cell membranes (MBs) from ovarian carcinoma OVMz cells. EVs were enriched in specific markers, including Tsg101, CD63, CD9, annexin-I, and MBs contained markers of cellular membrane compartments, including calnexin, GRASP65, GS28, LAMP-1, and L1CAM. The glycoprotein galectin-3 binding protein (LGALS3BP) was strongly enriched in EVs and it contained sialylated complex N-glycans. Lectin blotting with a panel of lectins showed that EVs had specific glycosignatures relative to MBs. Furthermore, the presence of glycoproteins bearing complex N-glycans with \u03b12,3-linked sialic acid, fucose, bisecting-GlcNAc and LacdiNAc structures, and O-glycans with the T-antigen were detected. The inhibition of N-glycosylation processing from high mannose to complex glycans using kifunensine caused changes in the composition of EVs and induced a decrease of several glycoproteins. In conclusion, the results showed that glycosignatures of EVs were specific and altered glycosylation within the cell affected the composition and\/or dynamics of EVs release. Furthermore, the identified glycosignatures of EVs could provide novel biomarkers for ovarian cancer.<\/jats:p>","DOI":"10.3390\/biom5031741","type":"journal-article","created":{"date-parts":[[2015,8,5]],"date-time":"2015-08-05T03:18:55Z","timestamp":1438744735000},"page":"1741-1761","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures"],"prefix":"10.3390","volume":"5","author":[{"given":"Joana","family":"Gomes","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, Oeiras 2780-157, Portugal"}]},{"given":"Patr\u00edcia","family":"Gomes-Alves","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, Oeiras 2780-157, Portugal"},{"name":"BET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras 2780-157, Portugal"}]},{"given":"Sofia","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, Oeiras 2780-157, Portugal"},{"name":"BET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras 2780-157, Portugal"}]},{"given":"Cristina","family":"Peixoto","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, Oeiras 2780-157, Portugal"},{"name":"BET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras 2780-157, Portugal"}]},{"given":"Paula","family":"Alves","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, Oeiras 2780-157, Portugal"},{"name":"BET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras 2780-157, Portugal"}]},{"given":"Peter","family":"Altevogt","sequence":"additional","affiliation":[{"name":"Skin Cancer Unit, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany"},{"name":"Department of Dermatology, Venereology and Allergology, University Medical Center Mannheim,  Ruprecht-Karl University of Heidelberg, Mannheim 68135, Germany"}]},{"given":"Julia","family":"Costa","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, Oeiras 2780-157, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2015,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev-cellbio-101512-122326","article-title":"Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles","volume":"30","author":"Colombo","year":"2014","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.molmed.2014.03.002","article-title":"Extracellular vesicles: Emerging targets for cancer therapy","volume":"20","author":"Vader","year":"2014","journal-title":"Trends Mol. Med."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Taylor, D.D., and Shah, S. (2015). Methods of isolating extracellular vesicles impact down-stream analyses of their cargoes. Methods.","DOI":"10.1016\/j.ymeth.2015.02.019"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Marleau, A.M., Chen, C.S., Joyce, J.A., and Tullis, R.H. (2012). Exosome removal as a therapeutic adjuvant in cancer. J. Transl. Med.","DOI":"10.1186\/1479-5876-10-134"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Witwer, K.W., Buzas, E.I., Bemis, L.T., Bora, A., Lasser, C., Lotvall, J., Nolte-\u2019t Hoen, E.N., Piper, M.G., Sivaraman, S., and Skog, J. (2013). Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J. Extracell. Vesicles.","DOI":"10.3402\/jev.v2i0.20360"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e1001450","DOI":"10.1371\/journal.pbio.1001450","article-title":"Vesiclepedia: A compendium for extracellular vesicles with continuous community annotation","volume":"10","author":"Kalra","year":"2012","journal-title":"PLoS Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1146\/annurev-cellbio-092910-154152","article-title":"Multivesicular body morphogenesis","volume":"28","author":"Hanson","year":"2012","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_8","first-page":"5553","article-title":"Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles","volume":"126","author":"Colombo","year":"2013","journal-title":"J. Cell Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1146\/annurev.cellbio.19.111301.153609","article-title":"Tetraspanin proteins mediate cellular penetration, invasion, and fusion events and define a novel type of membrane microdomain","volume":"19","author":"Hemler","year":"2003","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1083\/jcb.200108061","article-title":"Specific tetraspanin functions","volume":"155","author":"Hemler","year":"2001","journal-title":"J. Cell Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4143","DOI":"10.1242\/jcs.114.23.4143","article-title":"Complexes of tetraspanins with integrins: More than meets the eye","volume":"114","author":"Berditchevski","year":"2001","journal-title":"J. Cell Sci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Andreu, Z., and Yanez-Mo, M. (2014). Tetraspanins in extracellular vesicle formation and function. Front. Immunol.","DOI":"10.3389\/fimmu.2014.00442"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1111\/j.1600-0854.2009.00920.x","article-title":"Multivesicular endosome biogenesis in the absence of ESCRTs","volume":"10","author":"Stuffers","year":"2009","journal-title":"Traffic"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11649","DOI":"10.1074\/jbc.M112.445304","article-title":"The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes","volume":"288","author":"Jorge","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.bbalip.2013.10.004","article-title":"Exosomes as new vesicular lipid transporters involved in cell-cell communication and various pathophysiologies","volume":"1841","author":"Record","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1126\/science.1153124","article-title":"Ceramide triggers budding of exosome vesicles into multivesicular endosomes","volume":"319","author":"Trajkovic","year":"2008","journal-title":"Science"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1002\/path.2145","article-title":"Packaging of prions into exosomes is associated with a novel pathway of PrP processing","volume":"211","author":"Vella","year":"2007","journal-title":"J. Pathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1038\/nchembio.151","article-title":"HIV-1 and microvesicles from T cells share a common glycome, arguing for a common origin","volume":"5","author":"Krishnamoorthy","year":"2009","journal-title":"Nat. Chem. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4624","DOI":"10.1021\/pr200434y","article-title":"Identification of a conserved glycan signature for microvesicles","volume":"10","author":"Batista","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Escrevente, C., Keller, S., Altevogt, P., and Costa, J. (2011). Interaction and uptake of exosomes by ovarian cancer cells. BMC Cancer, 11.","DOI":"10.1186\/1471-2407-11-108"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e78631","DOI":"10.1371\/journal.pone.0078631","article-title":"Sialoglycoproteins and N-glycans from secreted exosomes of ovarian carcinoma cells","volume":"8","author":"Escrevente","year":"2013","journal-title":"PLoS ONE"},{"key":"ref_22","first-page":"103","article-title":"Tumor cell-derived exosomes: A message in a bottle","volume":"1826","author":"Kharaziha","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1002\/prca.201400114","article-title":"Exosomes in bodily fluids are a highly stable resource of disease biomarkers","volume":"9","author":"Boukouris","year":"2015","journal-title":"Proteom. Clin. Appl."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"769","DOI":"10.2217\/bmm.13.63","article-title":"Exosomes: The future of biomarkers in medicine","volume":"7","author":"Properzi","year":"2013","journal-title":"Biomark. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"32526","DOI":"10.1074\/jbc.M114.606269","article-title":"Complex N-linked glycans serve as a determinant for exosome\/microvesicle cargo recruitment","volume":"289","author":"Liang","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.1158\/1078-0432.CCR-04-1688","article-title":"Cleavage of L1 in exosomes and apoptotic membrane vesicles released from ovarian carcinoma cells","volume":"11","author":"Gutwein","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1093\/emboj\/17.6.1606","article-title":"Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin","volume":"17","author":"Sasaki","year":"1998","journal-title":"EMBO J."},{"key":"ref_28","unstructured":"Varki, A., Cummings, R.D., Esko, J.D., Freeze, H.H., Stanley, P., Bertozzi, C.R., Hart, G.W., and Etzler, M.E. (2009). Essentials of Glycobiology, Cold Spring Harbor Laboratory Press. [2nd ed.]."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1002\/pmic.200900537","article-title":"Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis","volume":"10","author":"Abbott","year":"2010","journal-title":"Proteomics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1021\/pr501174p","article-title":"Glycomic analysis of membrane glycoproteins with bisecting glycosylation from ovarian cancer tissues reveals novel structures and functions","volume":"14","author":"Allam","year":"2015","journal-title":"J. Proteome Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1093\/glycob\/cwq170","article-title":"N-Glycosylation of total cellular glycoproteins from the human ovarian carcinoma SKOV3 cell line and of recombinantly expressed human erythropoietin","volume":"21","author":"Machado","year":"2011","journal-title":"Glycobiology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1182\/blood-2009-07-231449","article-title":"Galectin-5 is bound onto the surface of rat reticulocyte exosomes and modulates vesicle uptake by macrophages","volume":"115","author":"Barres","year":"2010","journal-title":"Blood"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1002\/pros.21287","article-title":"Co-purification of Mac-2 binding protein with galectin-3 and association with prostasomes in human semen","volume":"71","author":"Block","year":"2011","journal-title":"Prostate"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1111\/j.1600-0854.2009.00960.x","article-title":"The role of galectins in protein trafficking","volume":"10","author":"Delacour","year":"2009","journal-title":"Traffic"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3636","DOI":"10.1091\/mbc.e12-04-0329","article-title":"Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism","volume":"23","author":"Mo","year":"2012","journal-title":"Mol. Biol. Cell"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4531","DOI":"10.1096\/fj.11-185991","article-title":"Mature N-linked glycans facilitate UT-A1 urea transporter lipid raft compartmentalization","volume":"25","author":"Chen","year":"2011","journal-title":"FASEB J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nrurol.2014.301","article-title":"The emerging role of extracellular vesicles as biomarkers for urogenital cancers","volume":"11","author":"Nawaz","year":"2014","journal-title":"Nat. Rev. Urol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1002\/pmic.201300389","article-title":"The roles of extracellular vesicles in cancer biology: Toward the development of novel cancer biomarkers","volume":"14","author":"Katsuda","year":"2014","journal-title":"Proteomics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/sj.embor.7400705","article-title":"Mucin-type O-glycans in human colon and breast cancer: Glycodynamics and functions","volume":"7","author":"Brockhausen","year":"2006","journal-title":"EMBO Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e74801","DOI":"10.1371\/journal.pone.0074801","article-title":"Surface glycosylation profiles of urine extracellular vesicles","volume":"8","author":"Gerlach","year":"2013","journal-title":"PLoS ONE"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1021\/pr200711w","article-title":"Differential glycomics of epithelial membrane glycoproteins from urinary exovesicles reveals shifts toward complex-type N-glycosylation in classical galactosemia","volume":"11","author":"Staubach","year":"2012","journal-title":"J. Proteome Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1038\/nbt.2886","article-title":"Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor","volume":"32","author":"Im","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.ygyno.2007.08.064","article-title":"Malignant ascites-derived exosomes of ovarian carcinoma patients contain CD24 and EpCAM","volume":"107","author":"Runz","year":"2007","journal-title":"Gynecol. Oncol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1023\/B:GLYC.0000014085.00706.d4","article-title":"90K (Mac-2 BP) and galectins in tumor progression and metastasis","volume":"19","author":"Grassadonia","year":"2004","journal-title":"Glycoconj. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1038\/ncb1800","article-title":"Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers","volume":"10","author":"Skog","year":"2008","journal-title":"Nat. Cell Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1038\/nm.2994","article-title":"Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy","volume":"18","author":"Shao","year":"2012","journal-title":"Nat. Med."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Colombo, E., Borgiani, B., Verderio, C., and Furlan, R. (2012). Microvesicles: Novel biomarkers for neurological disorders. Front. Physiol.","DOI":"10.3389\/fphys.2012.00063"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2667","DOI":"10.1007\/s00018-011-0689-3","article-title":"Membrane vesicles, current state-of-the-art: Emerging role of extracellular vesicles","volume":"68","author":"Gyorgy","year":"2011","journal-title":"Cell Mol. Life Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"11172","DOI":"10.1073\/pnas.0603838103","article-title":"Alzheimer\u2019s disease beta-amyloid peptides are released in association with exosomes","volume":"103","author":"Rajendran","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3842","DOI":"10.1074\/jbc.M111.277061","article-title":"Exosome-associated tau is secreted in tauopathy models and is selectively phosphorylated in cerebrospinal fluid in early Alzheimer disease","volume":"287","author":"Saman","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1007\/s00401-014-1314-y","article-title":"Plasma exosomal alpha-synuclein is likely CNS-derived and increased in Parkinson\u2019s disease","volume":"128","author":"Shi","year":"2014","journal-title":"Acta Neuropathol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.neulet.2007.09.024","article-title":"Evidence for secretion of Cu, Zn superoxide dismutase via exosomes from a cell model of amyotrophic lateral sclerosis","volume":"428","author":"Gomes","year":"2007","journal-title":"Neurosci. Lett."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3620","DOI":"10.1073\/pnas.1312245111","article-title":"Intercellular propagated misfolding of wild-type Cu\/Zn superoxide dismutase occurs via exosome-dependent and -independent mechanisms","volume":"111","author":"Grad","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/ncb1596","article-title":"Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells","volume":"9","author":"Valadi","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1038\/ncb1725","article-title":"Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells","volume":"10","author":"Meehan","year":"2008","journal-title":"Nat. Cell Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/nrn3887","article-title":"Spreading of pathology in neurodegenerative diseases: A focus on human studies","volume":"16","author":"Brettschneider","year":"2015","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.molmed.2014.04.003","article-title":"The role of extracellular vesicles in the progression of neurodegenerative disease and cancer","volume":"20","author":"Candelario","year":"2014","journal-title":"Trends Mol. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1146\/annurev-physiol-021014-071641","article-title":"Exosomes: Vehicles of intercellular signaling, biomarkers, and vectors of cell therapy","volume":"77","author":"Kourembanas","year":"2015","journal-title":"Annu. Rev. Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Mulcahy, L.A., Pink, R.C., and Carter, D.R. (2014). Routes and mechanisms of extracellular vesicle uptake. J. Extracell. Vesicles.","DOI":"10.3402\/jev.v3.24641"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1182\/blood-2013-03-489732","article-title":"CD169 mediates the capture of exosomes in spleen and lymph node","volume":"123","author":"Saunderson","year":"2014","journal-title":"Blood"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"17380","DOI":"10.1073\/pnas.1304266110","article-title":"Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity","volume":"110","author":"Christianson","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Franzen, C.A., Simms, P.E., van Huis, A.F., Foreman, K.E., Kuo, P.C., and Gupta, G.N. (2014). Characterization of uptake and internalization of exosomes by bladder cancer cells. Biomed. Res. Int.","DOI":"10.1155\/2014\/619829"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1365-2567.2006.02483.x","article-title":"Mature dendritic cells pulsed with exosomes stimulate efficient cytotoxic T-lymphocyte responses and antitumour immunity","volume":"120","author":"Hao","year":"2007","journal-title":"Immunology"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/nrd3978","article-title":"Extracellular vesicles: Biology and emerging therapeutic opportunities","volume":"12","author":"Mager","year":"2013","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/nbt.1807","article-title":"Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes","volume":"29","author":"Seow","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1038\/mt.2011.303","article-title":"Microvesicle-associated AAV vector as a novel gene delivery system","volume":"20","author":"Maguire","year":"2012","journal-title":"Mol. Ther."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/mt.2012.180","article-title":"Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells","volume":"21","author":"Ohno","year":"2013","journal-title":"Mol. Ther."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1476","DOI":"10.1002\/mds.25978","article-title":"Systemic exosomal siRNA delivery reduced alpha-synuclein aggregates in brains of transgenic mice","volume":"29","author":"Cooper","year":"2014","journal-title":"Mov. Disord."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1038\/mt.2011.164","article-title":"Treatment of brain inflammatory diseases by delivering exosome encapsulated anti-inflammatory drugs from the nasal region to the brain","volume":"19","author":"Zhuang","year":"2011","journal-title":"Mol. Ther."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1002\/pmic.201400318","article-title":"Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome","volume":"15","author":"Serra","year":"2015","journal-title":"Proteomics"}],"container-title":["Biomolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2218-273X\/5\/3\/1741\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:50:11Z","timestamp":1760215811000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2218-273X\/5\/3\/1741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,4]]},"references-count":70,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2015,9]]}},"alternative-id":["biom5031741"],"URL":"https:\/\/doi.org\/10.3390\/biom5031741","relation":{},"ISSN":["2218-273X"],"issn-type":[{"value":"2218-273X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,4]]}}}