{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:17:42Z","timestamp":1776471462022,"version":"3.51.2"},"reference-count":72,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,3,23]],"date-time":"2019-03-23T00:00:00Z","timestamp":1553299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["POC-A1.1.4-E-2015 nr. 30\/01.09.2016"],"award-info":[{"award-number":["POC-A1.1.4-E-2015 nr. 30\/01.09.2016"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["NORTE-01-0145-FEDER-000004"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000004"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/BIO\/04469\/2019"],"award-info":[{"award-number":["UID\/BIO\/04469\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomolecules"],"abstract":"<jats:p>Tau is a microtubule-associated protein that promotes microtubule assembly and stability. This protein is implicated in several neurodegenerative diseases, including Alzheimer\u2019s. To date, the three-dimensional (3D) structure of tau has not been fully solved, experimentally. Even the most recent information is sometimes controversial in regard to how this protein folds, interacts, and behaves. Predicting the tau structure and its profile sheds light on the knowledge about its properties and biological function, such as the binding to microtubules (MT) and, for instance, the effect on ionic conductivity. Our findings on the tau structure suggest a disordered protein, with discrete portions of well-defined secondary structure, mostly at the microtubule binding region. In addition, the first molecular dynamics simulation of full-length tau along with an MT section was performed, unveiling tau structure when associated with MT and interaction sites. Electrostatics and conductivity were also examined to understand how tau affects the ions in the intracellular fluid environment. Our results bring a new insight into tau and tubulin MT proteins, their characteristics, and the structure\u2013function relationship.<\/jats:p>","DOI":"10.3390\/biom9030116","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T06:56:52Z","timestamp":1553497012000},"page":"116","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Electrostatics of Tau Protein by Molecular Dynamics"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1040-459X","authenticated-orcid":false,"given":"Tarsila","family":"Castro","sequence":"first","affiliation":[{"name":"Aurel Vlaicu University of Arad, Str. Elena Dr\u0103goi 2-4, RO-310330 Arad, Romania"},{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6983-0617","authenticated-orcid":false,"given":"Florentina-Daniela","family":"Munteanu","sequence":"additional","affiliation":[{"name":"Aurel Vlaicu University of Arad, Str. Elena Dr\u0103goi 2-4, RO-310330 Arad, Romania"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7204-2064","authenticated-orcid":false,"given":"Artur","family":"Cavaco-Paulo","sequence":"additional","affiliation":[{"name":"Aurel Vlaicu University of Arad, Str. Elena Dr\u0103goi 2-4, RO-310330 Arad, Romania"},{"name":"Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1073\/pnas.72.5.1858","article-title":"A protein factor essential for microtubule assembly","volume":"72","author":"Weingarten","year":"1975","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2739","DOI":"10.1083\/jcb.103.6.2739","article-title":"Tau protein function in living cells","volume":"103","author":"Drubin","year":"1986","journal-title":"J. Cell Biol."},{"key":"ref_3","first-page":"1381","article-title":"Tau consists of a set of proteins with repeated C-terminal microtubule-binding domains and variable N-terminal domains","volume":"9","author":"Himmler","year":"1989","journal-title":"Mol. Cell. 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