{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T04:41:49Z","timestamp":1774413709635,"version":"3.50.1"},"reference-count":33,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T00:00:00Z","timestamp":1672790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003549","name":"Hungarian Research Grants","doi-asserted-by":"publisher","award":["NKFIH K-128250"],"award-info":[{"award-number":["NKFIH K-128250"]}],"id":[{"id":"10.13039\/501100003549","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003549","name":"Hungarian Research Grants","doi-asserted-by":"publisher","award":["NKFIH K-142348"],"award-info":[{"award-number":["NKFIH 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Scholarship of the Hungarian Academy of Sciences","award":["NKFIH PD-131689"],"award-info":[{"award-number":["NKFIH PD-131689"]}]},{"name":"J\u00e1nos Bolyai Research Scholarship of the Hungarian Academy of Sciences","award":["EFOP-3.6.1-16-2016-00022"],"award-info":[{"award-number":["EFOP-3.6.1-16-2016-00022"]}]},{"name":"J\u00e1nos Bolyai Research Scholarship of the Hungarian Academy of Sciences","award":["\u00daNKP-21-5"],"award-info":[{"award-number":["\u00daNKP-21-5"]}]},{"name":"New National Excellence Program of the Ministry for Innovation and Technology","award":["NKFIH K-128250"],"award-info":[{"award-number":["NKFIH K-128250"]}]},{"name":"New National Excellence Program of the Ministry for Innovation and Technology","award":["NKFIH K-142348"],"award-info":[{"award-number":["NKFIH K-142348"]}]},{"name":"New National Excellence Program of the Ministry for Innovation and Technology","award":["NKFIH PD-131689"],"award-info":[{"award-number":["NKFIH PD-131689"]}]},{"name":"New National Excellence Program of the Ministry for Innovation and Technology","award":["EFOP-3.6.1-16-2016-00022"],"award-info":[{"award-number":["EFOP-3.6.1-16-2016-00022"]}]},{"name":"New National Excellence Program of the Ministry for Innovation and Technology","award":["\u00daNKP-21-5"],"award-info":[{"award-number":["\u00daNKP-21-5"]}]},{"name":"the European Reference Network for Rare and Complex Skin Diseases","award":["NKFIH K-128250"],"award-info":[{"award-number":["NKFIH K-128250"]}]},{"name":"the European Reference Network for Rare and Complex Skin Diseases","award":["NKFIH K-142348"],"award-info":[{"award-number":["NKFIH K-142348"]}]},{"name":"the European Reference Network for Rare and Complex Skin Diseases","award":["NKFIH PD-131689"],"award-info":[{"award-number":["NKFIH PD-131689"]}]},{"name":"the European Reference Network for Rare and Complex Skin Diseases","award":["EFOP-3.6.1-16-2016-00022"],"award-info":[{"award-number":["EFOP-3.6.1-16-2016-00022"]}]},{"name":"the European Reference Network for Rare and Complex Skin Diseases","award":["\u00daNKP-21-5"],"award-info":[{"award-number":["\u00daNKP-21-5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedicines"],"abstract":"<jats:p>Hidradenitis suppurativa (HS) is a Th1\/17-driven inflammatory skin disease of the apocrine gland-rich (AGR) skin regions, where keratinocytes seem to be the crucial drivers of the initial pathogenic steps. However, the possible role of permeability barrier alteration in activating keratinocytes during HS development has not been clarified. We compared the major permeability barrier elements of non-lesional HS (HS-NL; n = 10) and lesional HS (HS-L; n = 10) skin with healthy AGR regions (n = 10) via RT-qPCR and immunohistochemistry. Stratum corneum components related to cornified envelope formation, corneocyte desquamation and (corneo)desmosome organization were analyzed along with tight junction molecules and barrier alarmins. The permeability barrier function was also investigated with transepidermal water loss (TEWL) measurements (n = 16). Junction structures were also visualized using confocal microscopy. At the gene level, none of the investigated molecules were significantly altered in HS-NL skin, while 11 molecules changed significantly in HS-L skin versus control. At the protein level, the investigated molecules were similarly expressed in HS-NL and AGR skin. In HS-L skin, only slight changes were detected; however, differences did not show a unidirectional alteration, as KRT1 and KLK5 were detected in decreased levels, and KLK7, KRT6 and DSG1 in increased levels. No significant differences in TEWL or the expression of junction structures were assessed. Our findings suggest that the permeability barrier is not significantly damaged in HS skin and permeability barrier alterations are not the driver factors of keratinocyte activation in this disease.<\/jats:p>","DOI":"10.3390\/biomedicines11010127","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T05:02:30Z","timestamp":1672808550000},"page":"127","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["New Data on the Features of Skin Barrier in Hidradenitis Suppurativa"],"prefix":"10.3390","volume":"11","author":[{"given":"Orsolya","family":"Somogyi","sequence":"first","affiliation":[{"name":"Department of Dermatology, MTA Centre of Excellence, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"ELKH-DE Allergology Research Group, H-4032 Debrecen, Hungary"},{"name":"Petr\u00e1nyi Gyula Doctoral School, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"given":"Zsolt","family":"Dajnoki","sequence":"additional","affiliation":[{"name":"Department of Dermatology, MTA Centre of Excellence, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"ELKH-DE Allergology Research Group, H-4032 Debrecen, Hungary"}]},{"given":"Lilla","family":"Szab\u00f3","sequence":"additional","affiliation":[{"name":"Department of Dermatology, MTA Centre of Excellence, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"ELKH-DE Allergology Research Group, H-4032 Debrecen, Hungary"},{"name":"Petr\u00e1nyi Gyula Doctoral School, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"given":"Kriszti\u00e1n","family":"G\u00e1sp\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Dermatology, MTA Centre of Excellence, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"ELKH-DE Allergology Research Group, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8546-2148","authenticated-orcid":false,"given":"Zolt\u00e1n","family":"Hendrik","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1646-2608","authenticated-orcid":false,"given":"Christos C.","family":"Zouboulis","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg, D-06847 Dessau, Germany"}]},{"given":"Klaudia","family":"D\u00f3cs","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4635-6427","authenticated-orcid":false,"given":"P\u00e9ter","family":"Sz\u00fccs","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5594-0364","authenticated-orcid":false,"given":"Katalin","family":"Dull","sequence":"additional","affiliation":[{"name":"Department of Dermatology, MTA Centre of Excellence, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"}]},{"given":"D\u00e1niel","family":"T\u00f6r\u0151csik","sequence":"additional","affiliation":[{"name":"Department of Dermatology, MTA Centre of Excellence, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"ELKH-DE Allergology Research Group, H-4032 Debrecen, Hungary"}]},{"given":"Anik\u00f3","family":"Kapit\u00e1ny","sequence":"additional","affiliation":[{"name":"Department of Dermatology, MTA Centre of Excellence, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"ELKH-DE Allergology Research Group, H-4032 Debrecen, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2109-9014","authenticated-orcid":false,"given":"Andrea","family":"Szegedi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, MTA Centre of Excellence, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary"},{"name":"ELKH-DE Allergology Research Group, H-4032 Debrecen, Hungary"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1001\/jamadermatol.2021.1677","article-title":"Prevalence of Hidradenitis Suppurativa: A Systematic Review and Meta-regression Analysis","volume":"157","author":"Jfri","year":"2021","journal-title":"JAMA Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1111\/exd.14214","article-title":"What causes hidradenitis suppurativa ?-15 years after","volume":"29","author":"Zouboulis","year":"2020","journal-title":"Exp. 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