{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:37:11Z","timestamp":1773322631769,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T00:00:00Z","timestamp":1694217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"IDI&amp;CA_Polytechnic Institute of Lisbon","award":["IPL\/2022\/CoIbu-Ils_ESTeSL"],"award-info":[{"award-number":["IPL\/2022\/CoIbu-Ils_ESTeSL"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedicines"],"abstract":"<jats:p>Background: Inflammatory bowel disease (IBD) is a public health issue with a growing prevalence, which can be divided into two phenotypes, namely Crohn\u2019s disease (CD) and ulcerative colitis (UC). Currently, used therapy is based only on symptomatic and\/or palliative pharmacological approaches. These treatments seek to induce and maintain remission of the disease and ameliorate its secondary effects; however, they do not modify or reverse the underlying pathogenic mechanism. Therefore, it is essential to investigate new potential treatments. Carbamylated erythropoietin (cEPO) results from the modification of the Erythropoietin (EPO) molecule, reducing cardiovascular-related side effects from the natural erythropoiesis stimulation. cEPO has been studied throughout several animal models, which demonstrated an anti-inflammatory effect by decreasing the production of several pro-inflammatory cytokines. Aim: This study aimed to evaluate the efficacy and safety of cEPO in a chronic TNBS-induced colitis model in rodents. Methods: Experimental colitis was induced by weekly intrarectal (IR) administrations of 1% TNBS for 5 weeks in female CD-1 mice. Then, the mice were treated with 500 IU\/kg\/day or 1000 IU\/kg\/day of cEPO through intraperitoneal injections for 14 days. Results: cEPO significantly reduced the concentration of alkaline phosphatase (ALP), fecal hemoglobin, tumor necrosis factor (TNF)-\u03b1, and interleukin (IL)-10. Also, it demonstrated a beneficial influence on the extra-intestinal manifestations, with the absence of significant side effects of its use. Conclusion: Considering the positive results from cEPO in this experiment, it may arise as a new possible pharmacological approach for the future management of IBD.<\/jats:p>","DOI":"10.3390\/biomedicines11092497","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T09:05:17Z","timestamp":1694423117000},"page":"2497","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Carbamylated Erythropoietin in a Chronic Model of TNBS-Induced Colitis"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7049-2512","authenticated-orcid":false,"given":"In\u00eas","family":"Silva","sequence":"first","affiliation":[{"name":"H&TRC\u2014Health & Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"},{"name":"iMed.ULisboa, Faculdade de Farm\u00e1cia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"given":"M\u00e1rio","family":"Gomes","sequence":"additional","affiliation":[{"name":"H&TRC\u2014Health & Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"},{"name":"Centro de Qu\u00edmica Estrutural, Institute of Molecular Sciences, Instituto Superior T\u00e9cnico, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]},{"given":"Carolina","family":"Al\u00edpio","sequence":"additional","affiliation":[{"name":"H&TRC\u2014Health & Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"}]},{"given":"J\u00e9ssica","family":"Vitoriano","sequence":"additional","affiliation":[{"name":"H&TRC\u2014Health & Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8283-3174","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Estarreja","sequence":"additional","affiliation":[{"name":"H&TRC\u2014Health & Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0013-3140","authenticated-orcid":false,"given":"Priscila","family":"Mendes","sequence":"additional","affiliation":[{"name":"H&TRC\u2014Health & Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"}]},{"given":"Rui","family":"Pinto","sequence":"additional","affiliation":[{"name":"iMed.ULisboa, Faculdade de Farm\u00e1cia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"},{"name":"Joaquim Chaves Sa\u00fade, Laborat\u00f3rio de An\u00e1lises Cl\u00ednicas, Miraflores, 1495-069 Alg\u00e9s, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3204-3772","authenticated-orcid":false,"given":"Vanessa","family":"Mateus","sequence":"additional","affiliation":[{"name":"H&TRC\u2014Health & Technology Research Center, ESTeSL\u2014Escola Superior de Tecnologia da Sa\u00fade, Instituto Polit\u00e9cnico de Lisboa, 1990-096 Lisbon, Portugal"},{"name":"iMed.ULisboa, Faculdade de Farm\u00e1cia, Universidade de Lisboa, 1349-017 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1016\/S0140-6736(17)32448-0","article-title":"Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: A systematic review of population-based studies","volume":"390","author":"Ng","year":"2017","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1016\/j.cgh.2020.01.028","article-title":"Changing Global Epidemiology of Inflammatory Bowel Diseases: Sustaining Health Care Delivery Into the 21st Century","volume":"18","author":"Ananthakrishnan","year":"2020","journal-title":"Clin. 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