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the adjunctive procedures to accelerate orthodontic tooth movement (OTM), ultrasound (US) is a nonsurgical form of mechanical stimulus that has been explored as an alternative to the currently available treatments. This study aimed to clarify the role of US in OTM by exploring different stimulation parameters and their effects on the biological responses of cells involved in OTM. Human fetal osteoblasts and periodontal ligament fibroblasts cell lines were stimulated with US at 1.0 and 1.5 MHz central frequencies and power densities of 30 and 60 mW\/cm2 in continuous mode for 5 and 10 min. Cellular proliferation, metabolic activity and protein expression were analyzed. The US parameters that significantly improved the metabolic activity were 1.0 MHz at 30 mW\/cm2 for 5 min and 1.0 MHz at 60 mW\/cm2 for 5 and 10 min for osteoblasts; and 1.0 MHz at 30 mW\/cm2 for 5 min and 1.5 MHz at 60 mW\/cm2 for 5 and 10 min for fibroblasts. By stimulating with these parameters, the expression of alkaline phosphatase was maintained, while osteoprotegerin synthesis was induced after three days of US stimulation. The US stimulation improved the biological activity of both osteoblasts and periodontal ligament fibroblasts, inducing their osteogenic differentiation.<\/jats:p>","DOI":"10.3390\/biomedicines12020361","type":"journal-article","created":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T08:41:43Z","timestamp":1707122503000},"page":"361","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts"],"prefix":"10.3390","volume":"12","author":[{"given":"Selma","family":"Pascoal","sequence":"first","affiliation":[{"name":"UNIPRO\u2014Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences (IUCS), CESPU, 4585-116 Gandra, Portugal"},{"name":"Center for MicroElectroMechanical Systems (CMEMS), University of Minho, Campus Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9617-5910","authenticated-orcid":false,"given":"Sofia","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Center for MicroElectroMechanical Systems (CMEMS), University of Minho, Campus Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4116-6237","authenticated-orcid":false,"given":"Francisca","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Center for MicroElectroMechanical Systems (CMEMS), University of Minho, Campus Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"},{"name":"ICVS\/3B\u2019s\u2014PT Government Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5630-6621","authenticated-orcid":false,"given":"Jorge","family":"Padr\u00e3o","sequence":"additional","affiliation":[{"name":"Center for Textile Science and Technology (2C2T), Department of Textile Engineering, University of Minho, Campus of Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"}]},{"given":"Rita","family":"Costa","sequence":"additional","affiliation":[{"name":"UNIPRO\u2014Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences (IUCS), CESPU, 4585-116 Gandra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5299-4164","authenticated-orcid":false,"given":"Andrea","family":"Zille","sequence":"additional","affiliation":[{"name":"Center for Textile Science and Technology (2C2T), Department of Textile Engineering, University of Minho, Campus of Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8962-0710","authenticated-orcid":false,"given":"Susana O.","family":"Catarino","sequence":"additional","affiliation":[{"name":"Center for MicroElectroMechanical Systems (CMEMS), University of Minho, Campus Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"given":"Filipe S.","family":"Silva","sequence":"additional","affiliation":[{"name":"Center for MicroElectroMechanical Systems (CMEMS), University of Minho, Campus Azur\u00e9m, 4800-058 Guimar\u00e3es, Portugal"},{"name":"LABBELS\u2014Associate Laboratory, 4710-057 Braga, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0012-6626","authenticated-orcid":false,"given":"Teresa","family":"Pinho","sequence":"additional","affiliation":[{"name":"UNIPRO\u2014Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences (IUCS), CESPU, 4585-116 Gandra, Portugal"},{"name":"IBMC\u2014Instituto Biologia Molecular e Celular, i3S\u2014Inst. 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Vol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0021-9290(99)00171-2","article-title":"Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity","volume":"33","author":"Kaspar","year":"2000","journal-title":"J. Biomech."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s00774-004-0587-y","article-title":"Effects of mechanical strain on proliferation and differentiation of bone marrow stromal cell line ST2","volume":"23","author":"Koike","year":"2005","journal-title":"J. Bone Miner. Metab."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1089\/107632701753337672","article-title":"Frequency- and Duration-Dependent Effects of Cyclic Pressure on Select Bone Cell Functions","volume":"7","author":"Nagatomi","year":"2001","journal-title":"Tissue Eng."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s00223-002-2027-0","article-title":"Mechanical Control of Human Osteoblast Apoptosis and Proliferation in Relation to Differentiation","volume":"72","author":"Weyts","year":"2003","journal-title":"Calcif. Tissue Int."},{"key":"ref_15","first-page":"225","article-title":"Osteoblasts increase their rate of division and align in response to cyclic, mechanical tension in vitro","volume":"4","author":"Buckley","year":"1988","journal-title":"Bone Miner."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1097\/00003086-199405000-00029","article-title":"Delayed Unions and Nonunions of Open Tibial Fractures. Correlation with arteriography results","volume":"302","author":"Dickson","year":"1994","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/BF00436764","article-title":"The stimulation of bone growth by ultrasound","volume":"101","author":"Duarte","year":"1983","journal-title":"Arch. Orthop. Trauma Surg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s002230010031","article-title":"Acceleration of Fresh Fracture Repair Using the Sonic Accelerated Fracture Healing System (SAFHS): A Review","volume":"66","author":"Warden","year":"2000","journal-title":"Calcif. Tissue Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.ajodo.2019.01.027","article-title":"Low-intensity pulsed ultrasound enhances the rate of lateral tooth movement and compensatory bone formation in rats","volume":"157","author":"Arai","year":"2020","journal-title":"Am. J. Orthod. Dentofac. Orthop."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.archoralbio.2016.06.007","article-title":"Effect of low-intensity pulsed ultrasound on the activity of osteoclasts: An in vitro study","volume":"70","author":"Feres","year":"2015","journal-title":"Arch. Oral Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"25","DOI":"10.4103\/ams.ams_57_17","article-title":"Effect of Low-intensity Pulsed Ultrasound on Postorthognathic Surgery Healing Process","volume":"7","author":"Tehranchi","year":"2017","journal-title":"Ann. Maxillofac. Surg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1902\/jop.2008.080081","article-title":"Effects of Ultrasound on the Proliferation and Differentiation of Cementoblast Lineage Cells","volume":"79","author":"Inubushi","year":"2008","journal-title":"J. Periodontol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1089\/rej.2007.0593","article-title":"Therapeutic Ultrasound Applications in Craniofacial Growth, Healing and Tissue Engineering","volume":"10","year":"2007","journal-title":"Rejuvenation Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106167","DOI":"10.1016\/j.ultras.2020.106167","article-title":"Therapeutic ultrasound experiments in vitro: Review of factors influencing outcomes and reproducibility","volume":"107","author":"Snehota","year":"2020","journal-title":"Ultrasonics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1093\/abbs\/gmn012","article-title":"Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts","volume":"41","author":"Suzuki","year":"2009","journal-title":"Acta Biochim. Biophys. Sin."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"119","DOI":"10.18521\/ktd.510808","article-title":"Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasound on Orthodontic Tooth Movement","volume":"11","author":"Cesur","year":"2019","journal-title":"Konuralp Tip Derg."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Wei, Y., and Guo, Y. (2022). Clinical Applications of Low-Intensity Pulsed Ultrasound and Its Underlying Mechanisms in Dentistry. Appl. Sci., 12.","DOI":"10.3390\/app122311898"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/s13287-020-01732-5","article-title":"Low-intensity pulsed ultrasound upregulates osteogenesis under inflammatory conditions in periodontal ligament stem cells through unfolded protein response","volume":"11","author":"Li","year":"2020","journal-title":"Stem Cell Res. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/jre.12696","article-title":"LIPUS inhibited the expression of inflammatory factors and promoted the osteogenic differentiation capacity of hPDLCs by inhibiting the NF-\u03baB signaling pathway","volume":"55","author":"Liu","year":"2020","journal-title":"J. Periodontal Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.bone.2013.01.021","article-title":"Ultrasound stimulation attenuates resorption of tooth root induced by experimental force application","volume":"53","author":"Inubushi","year":"2013","journal-title":"Bone"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Xue, H., Zheng, J., Cui, Z., Bai, X., Li, G., Zhang, C., He, S., Li, W., Lajud, S.A., and Duan, Y. (2013). Low-Intensity Pulsed Ultrasound Accelerates Tooth Movement via Activation of the BMP-2 Signaling Pathway. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0068926"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/S0278-2391(99)90281-1","article-title":"In vitro effects of therapeutic ultrasound on cell proliferation, protein synthesis, and cytokine production by human fibroblasts, osteoblasts, and monocytes","volume":"57","author":"Doan","year":"1999","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/S8756-3282(02)00789-5","article-title":"Ultrasound stimulates nitric oxide and prostaglandin e 2 production by human osteoblasts","volume":"31","author":"Reher","year":"2002","journal-title":"Bone"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1093\/ejo\/cjx062","article-title":"Effects of low magnitude high frequency mechanical vibration combined with compressive force on human periodontal ligament cells in vitro","volume":"40","author":"Benjakul","year":"2017","journal-title":"Eur. J. Orthod."},{"key":"ref_35","first-page":"19","article-title":"Anti-Inflammatory and Healing Effects of Pulsed Ultrasound Therapy on Fibroblasts","volume":"99","author":"Bertin","year":"2019","journal-title":"Am. J. Phys. Med. Rehabil."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1097\/PHM.0000000000001103","article-title":"Analysis of Cell Viability and Gene Expression After Continuous Ultrasound Therapy in L929 Fibroblast Cells","volume":"98","author":"Bertin","year":"2019","journal-title":"Am. J. Phys. Med. Rehabil."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.ultrasmedbio.2010.12.004","article-title":"Effects of Near-Field Ultrasound Stimulation on New Bone Formation and Osseointegration of Dental Titanium Implants In Vitro and In Vivo","volume":"37","author":"Hsu","year":"2011","journal-title":"Ultrasound Med. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1002\/jbmr.5650070216","article-title":"Relationship between collagen synthesis and expression of the osteoblast phenotype in MC3T3-E1 cells","volume":"7","author":"Franceschi","year":"1992","journal-title":"J. Bone Miner. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1089\/scd.2008.0340","article-title":"Umbilical Cord Mesenchymal Stem Cells: Role of Regulatory Genes in Their Differentiation to Osteoblasts","volume":"18","author":"Ciavarella","year":"2009","journal-title":"Stem Cells Dev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/j.archoralbio.2013.01.009","article-title":"Osteogenic differentiation of periodontal fibroblasts is dependent on the strength of mechanical strain","volume":"58","author":"Jacobs","year":"2013","journal-title":"Arch. Oral Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/j.lfs.2006.11.037","article-title":"Low-intensity pulsed ultrasound stimulates osteogenic differentiation in ROS 17\/2.8 cells","volume":"80","author":"Takayama","year":"2007","journal-title":"Life Sci."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Hu, B., Zhang, Y., Zhou, J., Li, J., Deng, F., Wang, Z., and Song, J. (2014). Low-Intensity Pulsed Ultrasound Stimulation Facilitates Osteogenic Differentiation of Human Periodontal Ligament Cells. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0095168"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s10561-022-10010-y","article-title":"Low-intensity pulsed ultrasound enhances immunomodulation and facilitates osteogenesis of human periodontal ligament stem cells by inhibiting the NF-\u03baB pathway","volume":"24","author":"Lin","year":"2023","journal-title":"Cell Tissue Bank."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhang, L., Ding, Y., Rao, G., and Miao, D. (2016). Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts. Braz. J. Med Biol. Res., 49.","DOI":"10.1590\/1414-431X20154324"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1111\/jre.12197","article-title":"Role of periodontal ligament fibroblasts in osteoclastogenesis: A review","volume":"50","author":"Sokos","year":"2015","journal-title":"J. Periodontal Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"5050","DOI":"10.1210\/endo.142.12.8536","article-title":"Minireview: The OPG\/RANKL\/RANK System","volume":"142","author":"Khosla","year":"2001","journal-title":"Endocrinology"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Tobeiha, M., Moghadasian, M.H., Amin, N., and Jafarnejad, S. (2020). RANKL\/RANK\/OPG Pathway: A Mechanism Involved in Exercise-Induced Bone Remodeling. BioMed Res. Int., 2020.","DOI":"10.1155\/2020\/6910312"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1601-6343.2008.00423.x","article-title":"Low-energy laser stimulates tooth movement velocity via expression of RANK and RANKL","volume":"11","author":"Fujita","year":"2008","journal-title":"Orthod. Craniofacial Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"498","DOI":"10.2319\/060809-318.1","article-title":"Comparison of Low-Intensity Pulsed Ultrasound and Pulsed Electromagnetic Field Treatments on OPG and RANKL Expression in Human Osteoblast-like Cells","volume":"80","author":"Borsje","year":"2010","journal-title":"Angle Orthod."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1002\/jum.15738","article-title":"Low-Intensity Pulsed Ultrasound Stimulation for Bone Fractures Healing","volume":"41","author":"Palanisamy","year":"2022","journal-title":"J. Ultrasound Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"8252696","DOI":"10.1155\/2022\/8252696","article-title":"Human Interleukin-1\u03b2 Profile and Self-Reported Pain Monitoring Using Clear Aligners with or without Acceleration Techniques: A Case Report and Investigational Study","volume":"2022","author":"Pascoal","year":"2022","journal-title":"Int. J. Dent."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.ajodo.2017.10.017","article-title":"Effect of supplemental vibration on orthodontic treatment with aligners: A randomized trial","volume":"153","author":"Katchooi","year":"2018","journal-title":"Am. J. Orthod. Dentofac. Orthop."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1093\/ejo\/cjaa026","article-title":"Biomarkers of orthodontic tooth movement with fixed appliances and vibration appliance therapy: A pilot study","volume":"42","author":"Reiss","year":"2020","journal-title":"Eur. J. Orthod."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1093\/rheumatology\/40.12.1331","article-title":"Therapeutic ultrasound in soft tissue lesions","volume":"40","author":"Speed","year":"2001","journal-title":"Rheumatology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1055\/s-0034-1544104","article-title":"An Evaluation of the Effect of Therapeutic Ultrasound on Healing of Mandibular Fracture","volume":"8","author":"Patel","year":"2014","journal-title":"Craniomaxillofacial Trauma Reconstr."}],"container-title":["Biomedicines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2227-9059\/12\/2\/361\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:54:25Z","timestamp":1760104465000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2227-9059\/12\/2\/361"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,3]]},"references-count":55,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["biomedicines12020361"],"URL":"https:\/\/doi.org\/10.3390\/biomedicines12020361","relation":{},"ISSN":["2227-9059"],"issn-type":[{"value":"2227-9059","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,3]]}}}