{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:40:19Z","timestamp":1760146819154,"version":"build-2065373602"},"reference-count":74,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,15]],"date-time":"2024-12-15T00:00:00Z","timestamp":1734220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Patr\u00edcia Sousa","award":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"],"award-info":[{"award-number":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"]}]},{"name":"Bruna Lopes","award":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"],"award-info":[{"award-number":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"]}]},{"name":"Ana Catarina Sousa","award":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"],"award-info":[{"award-number":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"]}]},{"name":"Al\u00edcia Moreira","award":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"],"award-info":[{"award-number":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"]}]},{"name":"Andr\u00e9 Coelho","award":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"],"award-info":[{"award-number":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"]}]},{"name":"FCT\/MCTES","award":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"],"award-info":[{"award-number":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"]}]},{"name":"Olfabionerve\u2014Olfactory Mucosa Mesenchymal StemCells and Biomaterials Promoting Peripheral Nerve Regeneration","award":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"],"award-info":[{"award-number":["2023.00246.BD","2021.05265.BD","SFRH\/BD\/146689\/2019","2023.00544.BD","2023.00428.BD","UIDB\/00211\/2020","2022.04501.PTDC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedicines"],"abstract":"<jats:p>Background: Stem cells are capable of self-renewal and differentiation into various specialized cells, making them a potential therapeutic option in regenerative medicine. This study establishes a comprehensive methodology for isolating, culturing, and characterizing rat hair follicle stem cells. Methods and Results: Hair follicles were harvested from Sprague\u2013Dawley rats and subjected to two different isolation techniques. Immunohistochemical analysis and real-time PCR confirm the expression of specific surface markers and genes, validating the cells\u2019 identity. Growth kinetics, colony formation units (CFU), and tri-differentiation capacity were also assessed. Additionally, the cells\u2019 secretome was analyzed, regarding its content in biofactors with wound healing properties. Conclusions: These findings highlight the potential of these cells as a valuable cell source for skin regeneration applications. They contribute to advancing our understanding of stem cell applications in regenerative medicine and hold promise for therapeutic interventions in various clinical contexts, aligning with broader research on the diverse capabilities of hair follicle stem cells.<\/jats:p>","DOI":"10.3390\/biomedicines12122854","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T09:17:58Z","timestamp":1734340678000},"page":"2854","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation, Expansion, and Characterization of Rat Hair Follicle Stem Cells and Their Secretome: Insights into Wound Healing Potential"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2531-3196","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Sousa","sequence":"first","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8168-2415","authenticated-orcid":false,"given":"Bruna","family":"Lopes","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8800-2195","authenticated-orcid":false,"given":"Ana Catarina","family":"Sousa","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), 1300-477 Lisboa, Portugal"}]},{"given":"Andr\u00e9","family":"Coelho","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1347-8789","authenticated-orcid":false,"given":"Al\u00edcia","family":"de Sousa Moreira","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4319-2404","authenticated-orcid":false,"given":"Alexandra","family":"R\u00eama","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), 1300-477 Lisboa, Portugal"}]},{"given":"Maria","family":"Gon\u00e7alves-Maia","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal"},{"name":"Maia & Muller-Biotech, Rua Alfredo Allen, 455\/461, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2305-1631","authenticated-orcid":false,"given":"Irina","family":"Amorim","sequence":"additional","affiliation":[{"name":"Departamento de Patologia e Imunologia Molecular, ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto (UP), Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"Institute for Research and Innovation in Health (i3S), Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal"},{"name":"Institute of Molecular Pathology and Immunology, University of Porto (IPATIMUP), Rua J\u00falio Amaral de Carvalho 45, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1945-6377","authenticated-orcid":false,"given":"Rui","family":"Alvites","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. 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