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de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["APQ-01485-22"],"award-info":[{"award-number":["APQ-01485-22"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedicines"],"abstract":"<jats:p>Background\/Objectives: Pulmonary arterial hypertension (PAH) affects the pulmonary vasculature and cardiac function. While its impact on target organs has been extensively studied, little is known about its effects on highly vascularized organs, such as those from the male reproductive system. This study explores the impact of PAH on testis and epididymis, evaluating the potential role of combined exercise training as a non-pharmacological strategy to mitigate alterations in these organs. Methods: Male Wistar rats (n = 8\/group) were assigned to one of three groups: sedentary control, sedentary PAH, and exercise PAH. PAH was induced by monocrotaline administration (60 mg Kg\u22121, i.p). The exercise PAH group underwent three weeks of combined physical training, including treadmill aerobic activity and resistance training on a ladder. Testis and epididymis were analyzed histologically, histomorphometrically, and biochemically for antioxidant activity, oxidative stress markers, and sperm parameters. Results: Sedentary PAH animals showed reductions in body and epididymis weight, normal seminiferous tubule percentage, and testicular morphometric parameters. These changes led to disorganized seminiferous tubules and compromised sperm production and sperm count in the testis and epididymis. Combined physical training improved testicular morphometric alterations and increased sperm count in hypertensive animals. Conclusions: PAH negatively affects testicular structure and function, leading to low sperm production. Combined physical training mitigated these effects by preserving testicular architecture and improving reproductive parameters, though it appeared less effective for the epididymis. These findings suggest physical training as a potential therapeutic strategy to protect reproductive health in PAH.<\/jats:p>","DOI":"10.3390\/biomedicines13020410","type":"journal-article","created":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T09:29:26Z","timestamp":1739179766000},"page":"410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Pulmonary Arterial Hypertension-Induced Reproductive Damage: Effects of Combined Physical Training on Testicular and Epididymal Parameters in Rats"],"prefix":"10.3390","volume":"13","author":[{"given":"M\u00edrian Quint\u00e3o","family":"Assis","sequence":"first","affiliation":[{"name":"Department of General Biology, Federal University of Vi\u00e7osa, Vi\u00e7osa 36570-900, MG, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3012-1327","authenticated-orcid":false,"given":"Luciano Bernardes","family":"Leite","sequence":"additional","affiliation":[{"name":"Laboratory of Exercise Biology, Federal University of Vi\u00e7osa, Vi\u00e7osa 36570-900, MG, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6355-2492","authenticated-orcid":false,"given":"Luiz Ot\u00e1vio","family":"Guimar\u00e3es-Ervilha","sequence":"additional","affiliation":[{"name":"Department of General Biology, Federal University of Vi\u00e7osa, Vi\u00e7osa 36570-900, MG, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2203-436X","authenticated-orcid":false,"given":"Rui","family":"Ad\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, School of Medicine, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"CIBER Enfermedades Respiratorias (Ciberes), 28029 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n (IiSGM), 28009 Madrid, Spain"}]},{"given":"Emily Correna Carlo","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Vi\u00e7osa, Vi\u00e7osa 36570-900, MG, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4927-4024","authenticated-orcid":false,"given":"Ant\u00f4nio Jos\u00e9","family":"Natali","sequence":"additional","affiliation":[{"name":"Laboratory of Exercise Biology, Federal University of Vi\u00e7osa, Vi\u00e7osa 36570-900, MG, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7416-3529","authenticated-orcid":false,"given":"Mariana","family":"Machado-Neves","sequence":"additional","affiliation":[{"name":"Department of General Biology, Federal University of Vi\u00e7osa, Vi\u00e7osa 36570-900, MG, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2200879","DOI":"10.1183\/13993003.00879-2022","article-title":"2022 ESC\/ERS Guidelines for the diagnosis and treatment of pulmonary hypertension","volume":"61","author":"Humbert","year":"2023","journal-title":"Eur. 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