{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T17:27:07Z","timestamp":1776446827878,"version":"3.51.2"},"reference-count":118,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T00:00:00Z","timestamp":1715990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"INOU22-02A-Characterization of micro and nanoplastics in the province of Ourense water resources","award":["2022 K240 131H 647. 02"],"award-info":[{"award-number":["2022 K240 131H 647. 02"]}]},{"name":"INOU22-02A-Characterization of micro and nanoplastics in the province of Ourense water resources","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}]},{"name":"INOU22-02A-Characterization of micro and nanoplastics in the province of Ourense water resources","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]},{"name":"FCT","award":["2022 K240 131H 647. 02"],"award-info":[{"award-number":["2022 K240 131H 647. 02"]}]},{"name":"FCT","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}]},{"name":"FCT","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]},{"name":"University of Vigo\/CISUG","award":["2022 K240 131H 647. 02"],"award-info":[{"award-number":["2022 K240 131H 647. 02"]}]},{"name":"University of Vigo\/CISUG","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}]},{"name":"University of Vigo\/CISUG","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomimetics"],"abstract":"<jats:p>The overproduction and mismanagement of plastics has led to the accumulation of these materials in the environment, particularly in the marine ecosystem. Once in the environment, plastics break down and can acquire microscopic or even nanoscopic sizes. Given their sizes, microplastics (MPs) and nanoplastics (NPs) are hard to detect and remove from the aquatic environment, eventually interacting with marine organisms. This research mainly aimed to achieve the aggregation of micro- and nanoplastics (MNPs) to ease their removal from the marine environment. To this end, the size and stability of polystyrene (PS) MNPs were measured in synthetic seawater with the different components of the technology (ionic liquid and chitosan). The MPs were purchased in their plain form, while the NPs displayed amines on their surface (PS NP-NH2). The results showed that this technology promoted a significant aggregation of the PS NP-NH2, whereas, for the PS MPs, no conclusive results were found, indicating that the surface charge plays an essential role in the MNP aggregation process. Moreover, to investigate the toxicological potential of MNPs, a mussel species (M. galloprovincialis) was exposed to different concentrations of MPs and NPs, separately, with and without the technology. In this context, mussels were sampled after 7, 14, and 21 days of exposure, and the gills and digestive glands were collected for analysis of oxidative stress biomarkers and histological observations. In general, the results indicate that MNPs trigger the production of reactive oxygen species (ROS) in mussels and induce oxidative stress, making gills the most affected organ. Yet, when the technology was applied in moderate concentrations, NPs showed adverse effects in mussels. The histological analysis showed no evidence of MNPs in the gill\u2019s tissues.<\/jats:p>","DOI":"10.3390\/biomimetics9050303","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T06:31:58Z","timestamp":1716186718000},"page":"303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development of a New Aggregation Method to Remove Nanoplastics from the Ocean: Proof of Concept Using Mussel Exposure Tests"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0507-3394","authenticated-orcid":false,"given":"Antonio","family":"Cid-Samamed","sequence":"first","affiliation":[{"name":"Physical Chemistry Department, Faculty of Sciences, University of Vigo, Campus de As Lagoas S\/N, 32004 Ourense, Spain"}]},{"given":"Catarina S. E.","family":"Nunes","sequence":"additional","affiliation":[{"name":"i4HB\u2014Associate Laboratory Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"UCIBIO, Chemistry Department, NOVA School of Science & Technology, Universidade NOVA de Lisboa, 2819-516 Caparica, Portugal"}]},{"given":"Cristina","family":"Lomas Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Environmental Protection, Estaci\u00f3n Experimental del Zaid\u00edn, Consejo Superior de Investigaciones Cient\u00edficas, 18008 Granada, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-0366","authenticated-orcid":false,"given":"M\u00e1rio S.","family":"Diniz","sequence":"additional","affiliation":[{"name":"i4HB\u2014Associate Laboratory Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2819-516 Caparica, Portugal"},{"name":"UCIBIO, Chemistry Department, NOVA School of Science & Technology, Universidade NOVA de Lisboa, 2819-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"0116","DOI":"10.1038\/s41559-017-0116","article-title":"Interactions of Microplastic Debris throughout the Marine Ecosystem","volume":"1","author":"Galloway","year":"2017","journal-title":"Nat. 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