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We demonstrate the full manipulation of the sample and reagents on the DMF platform, resulting in the successful amplification of 90 pg of the target DNA (0.5 ng\/\u00b5L) in less than one hour. Furthermore, we test the efficiency of an innovative mixing strategy in DMF by employing two mixing methodologies onto the DMF droplets\u2014low frequency AC (alternating current) actuation as well as back-and-forth droplet motion\u2014which allows for improved fluorescence readouts. Fluorophore bleaching effects are minimized through on-chip sample partitioning by DMF processes and sequential droplet irradiation. Finally, LAMP reactions require only 2 \u00b5L volume droplets, which represents a 10-fold volume reduction in comparison to benchtop LAMP.<\/jats:p>","DOI":"10.3390\/bios12040201","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T21:40:14Z","timestamp":1648590014000},"page":"201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Digital Microfluidics-Powered Real-Time Monitoring of Isothermal DNA Amplification of Cancer Biomarker"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3359-3773","authenticated-orcid":false,"given":"Beatriz Jorge","family":"Coelho","sequence":"first","affiliation":[{"name":"Department of Materials Science, School of Science and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, Campus de Caparica, 2829-516 Caparica, Portugal"},{"name":"UCIBIO, I4HB, Life Sciences Department, School of Science and Technology, NOVA University of Lisbon, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"given":"Bruno","family":"Veigas","sequence":"additional","affiliation":[{"name":"AlmaScience, Campus da Caparica, 2829-519 Caparica, Portugal"}]},{"given":"Lu\u00eds","family":"Bettencourt","sequence":"additional","affiliation":[{"name":"Department of Materials Science, School of Science and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7350-649X","authenticated-orcid":false,"given":"Hugo","family":"\u00c1guas","sequence":"additional","affiliation":[{"name":"Department of Materials Science, School of Science and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"given":"Elvira","family":"Fortunato","sequence":"additional","affiliation":[{"name":"Department of Materials Science, School of Science and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1997-7669","authenticated-orcid":false,"given":"Rodrigo","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Materials Science, School of Science and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5255-7095","authenticated-orcid":false,"given":"Pedro V.","family":"Baptista","sequence":"additional","affiliation":[{"name":"UCIBIO, I4HB, Life Sciences Department, School of Science and Technology, NOVA University of Lisbon, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6608-3422","authenticated-orcid":false,"given":"Rui","family":"Igreja","sequence":"additional","affiliation":[{"name":"Department of Materials Science, School of Science and Technology, NOVA University of Lisbon and CEMOP\/UNINOVA, Campus de Caparica, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,28]]},"reference":[{"key":"ref_1","unstructured":"(2021, May 04). 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