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For understanding the pathophysiology processes involved in brain disorders, PC-12 cell line is extensively assessed in neuroscience research, including studies on neurotoxicity, neuroprotection, or neurosecretion. Various analytical technologies have been developed to investigate physicochemical processes and the biosensors based on optical and electrochemical techniques, among others, have been at the forefront of this development. This article summarizes the application of different biosensors in PC-12 cell cultures and presents the modern approaches employed in neuronal networks biosensing.<\/jats:p>","DOI":"10.3390\/bios12070500","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T11:37:08Z","timestamp":1657280228000},"page":"500","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["PC-12 Cell Line as a Neuronal Cell Model for Biosensing Applications"],"prefix":"10.3390","volume":"12","author":[{"given":"Daniela","family":"Oprea","sequence":"first","affiliation":[{"name":"National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2829-7163","authenticated-orcid":false,"given":"Caroline G.","family":"Sanz","sequence":"additional","affiliation":[{"name":"National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania"}]},{"given":"Madalina M.","family":"Barsan","sequence":"additional","affiliation":[{"name":"National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1848-6753","authenticated-orcid":false,"given":"Teodor Adrian","family":"Enache","sequence":"additional","affiliation":[{"name":"National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"328","DOI":"10.3389\/fcell.2020.00328","article-title":"In vitro Models of Neurodegenerative Diseases","volume":"8","author":"Slanzi","year":"2020","journal-title":"Front. 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