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The transducer\u2019s high selectivity is based on the specific interaction of a molecularly imprinted polymer (MIPs) design for IgG detection. The sensing scheme is based on differential refractometric measurements, including a correction system based on a non-imprinted polymer (NIP)-coated LPFG, allowing reliable and more sensitive measurements, improving the rejection of false positives in around 30%. The molecular imprinted binding sites were performed on the surface of a LPFG with a sensitivity of about 130 nm\/RIU and a FOM of 16 RIU\u22121. The low-cost and easy to build device was tested in a working range from 1 to 100 nmol\/L, revealing a limit of detection (LOD) and a sensitivity of 0.25 nmol\/L (0.037 \u00b5g\/mL) and 0.057 nm.L\/nmol, respectively. The sensor also successfully differentiates the target analyte from the other abundant elements that are present in the human blood plasma.<\/jats:p>","DOI":"10.3390\/bios12070515","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T20:52:41Z","timestamp":1657659161000},"page":"515","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1031-9261","authenticated-orcid":false,"given":"Jo\u00e3o P.","family":"Mendes","sequence":"first","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Qu\u00edmica UP (CIQUP)\u2014Instituto de Ci\u00eancias Moleculares (IMS), Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal"},{"name":"INESC TEC\u2014Instituto de Engenharia de Sistemas e Computadores, Tecnologia e Ci\u00eancia, Departamento de F\u00edsica e Astronomia, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal"},{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6205-9479","authenticated-orcid":false,"given":"Lu\u00eds C. 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S.","family":"Jorge","sequence":"additional","affiliation":[{"name":"INESC TEC\u2014Instituto de Engenharia de Sistemas e Computadores, Tecnologia e Ci\u00eancia, Departamento de F\u00edsica e Astronomia, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal"},{"name":"Departamento de F\u00edsica e Astronomia, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8392-9581","authenticated-orcid":false,"given":"Carlos M.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Qu\u00edmica UP (CIQUP)\u2014Instituto de Ci\u00eancias Moleculares (IMS), Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal"},{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.aca.2017.03.012","article-title":"Determination of nanomolar levels of reactive oxygen species in microorganisms and aquatic environments using a single nanoparticle-based optical sensor","volume":"967","author":"Kim","year":"2017","journal-title":"Anal. 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