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In this paper, a method for its effective analytical control in a milk sample was developed. To perform rapid (1 h) and accurate analysis, monodisperse Fe3O4@Au magnetic nanoparticles were synthesized and used in an electrochemical sandwich-type magnetic immunoassay. Screen-printed carbon electrodes (SPCE) were used as transducers, and electrochemical detection was performed by chronoamperometry using a secondary horseradish peroxidase-labeled antibody and 3,3\u2032,5,5\u2032-tetramethylbenzidine. This magnetic assay was used to determine the E. coli O157:H7 strain in the linear range from 20 to 2 \u00d7 106 CFU\/mL, with a limit of detection of 20 CFU\/mL. The selectivity of the assay was tested using Listeria monocytogenes p60 protein, and the applicability of the assay was assessed by analyzing a commercial milk sample, demonstrating the usefulness of the synthesized nanoparticles in the developed magnetic immunoassay.<\/jats:p>","DOI":"10.3390\/bios13050567","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T05:49:32Z","timestamp":1684734572000},"page":"567","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Fe3O4@Au Core\u2013Shell Magnetic Nanoparticles for the Rapid Analysis of E. coli O157:H7 in an Electrochemical Immunoassay"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1880-5961","authenticated-orcid":false,"given":"Shayesteh","family":"Bazsefidpar","sequence":"first","affiliation":[{"name":"Department of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c\/Juli\u00e1n Claver\u00eda 8, 33006 Oviedo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0628-2753","authenticated-orcid":false,"given":"Maria","family":"Freitas","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Instituto Superior de Engenharia do Porto, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9224-1917","authenticated-orcid":false,"given":"Clara R.","family":"Pereira","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2700-4944","authenticated-orcid":false,"given":"Gemma","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Department of Chemical and Environmental Engineering & Institute of Biotechnology of Asturias, University of Oviedo, c\/Juli\u00e1n Claver\u00eda 8, 33006 Oviedo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8356-858X","authenticated-orcid":false,"given":"Esther","family":"Serrano-Pertierra","sequence":"additional","affiliation":[{"name":"Department of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c\/Juli\u00e1n Claver\u00eda 8, 33006 Oviedo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1472-8866","authenticated-orcid":false,"given":"Henri P. A.","family":"Nouws","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Instituto Superior de Engenharia do Porto, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6980-0554","authenticated-orcid":false,"given":"Mar\u00eda","family":"Matos","sequence":"additional","affiliation":[{"name":"Department of Chemical and Environmental Engineering & Institute of Biotechnology of Asturias, University of Oviedo, c\/Juli\u00e1n Claver\u00eda 8, 33006 Oviedo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3924-776X","authenticated-orcid":false,"given":"Cristina","family":"Delerue-Matos","sequence":"additional","affiliation":[{"name":"REQUIMTE\/LAQV, Instituto Superior de Engenharia do Porto, Instituto Polit\u00e9cnico do Porto, Rua Dr. Ant\u00f3nio Bernardino de Almeida 431, 4249-015 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9776-9013","authenticated-orcid":false,"given":"Mar\u00eda Carmen","family":"Blanco-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Physical and Analytical Chemistry & Institute of Biotechnology of Asturias, University of Oviedo, c\/Juli\u00e1n Claver\u00eda 8, 33006 Oviedo, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e2212436119","DOI":"10.1073\/pnas.2212436119","article-title":"Escherichia coli O157:H7 Senses Microbiota-Produced Riboflavin to Increase Its Virulence in the Gut","volume":"119","author":"Liu","year":"2022","journal-title":"Proc. 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