{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:56:14Z","timestamp":1760144174131,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia, I.P. (FCT-MCTES)","award":["UIDP\/04378\/2020","UIDB\/04378\/2020","UIDB\/50006\/2020","UIDP\/50006\/2020","LA\/P\/0140\/2020","REQUIMTE 2022-06"],"award-info":[{"award-number":["UIDP\/04378\/2020","UIDB\/04378\/2020","UIDB\/50006\/2020","UIDP\/50006\/2020","LA\/P\/0140\/2020","REQUIMTE 2022-06"]}]},{"name":"LAQV","award":["UIDP\/04378\/2020","UIDB\/04378\/2020","UIDB\/50006\/2020","UIDP\/50006\/2020","LA\/P\/0140\/2020","REQUIMTE 2022-06"],"award-info":[{"award-number":["UIDP\/04378\/2020","UIDB\/04378\/2020","UIDB\/50006\/2020","UIDP\/50006\/2020","LA\/P\/0140\/2020","REQUIMTE 2022-06"]}]},{"name":"Erasmus+: Erasmus Mundus Program of the European Union","award":["UIDP\/04378\/2020","UIDB\/04378\/2020","UIDB\/50006\/2020","UIDP\/50006\/2020","LA\/P\/0140\/2020","REQUIMTE 2022-06"],"award-info":[{"award-number":["UIDP\/04378\/2020","UIDB\/04378\/2020","UIDB\/50006\/2020","UIDP\/50006\/2020","LA\/P\/0140\/2020","REQUIMTE 2022-06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Gold nanoparticles (AuNPs) exhibit improved optical and spectral properties compared to bulk materials, making them suitable for the detection of DNA, RNA, antigens, and antibodies. Here, we describe a simple, selective, and rapid non-cross linking detection assay, using approx. 35 nm spherical Au nanoprobes, for a common mutation occurring in exon 19 of the epidermal growth factor receptor (EGFR), associated with non-small-cell lung cancer cells. AuNPs were synthesized based on the seed-mediated growth method and functionalized with a specific 16 bp thiolated oligonucleotide using a pH-assisted method. Both AuNPs and Au nanoprobes proved to be highly stable and monodisperse through ultraviolet-visible spectrophotometry, dynamic light scattering (DLS), and electrophoretic light scattering (ELS). Our results indicate a detection limit of 1.5 \u00b5g mL\u22121 using a 0.15 nmol dm\u22123 Au nanoprobe concentration. In conclusion, this work presents an effective possibility for a straightforward, fast, and inexpensive alternative for the detection of DNA sequences related to lung cancer, leading to a potential platform for early diagnosis of lung cancer patients.<\/jats:p>","DOI":"10.3390\/bios14040162","type":"journal-article","created":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T11:11:09Z","timestamp":1712229069000},"page":"162","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gold Nanoprobes for Detection of a Crucial EGFR Deletion for Early Diagnosis of Non-Small-Cell Lung Cancer"],"prefix":"10.3390","volume":"14","author":[{"given":"Maria","family":"Enea","sequence":"first","affiliation":[{"name":"LAQV\/REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, Rua Campo Alegre, 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0574-6395","authenticated-orcid":false,"given":"Anupong","family":"Nuekaew","sequence":"additional","affiliation":[{"name":"LAQV\/REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, Rua Campo Alegre, 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5139-2871","authenticated-orcid":false,"given":"Ricardo","family":"Franco","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, 2819-516 Caparica, Portugal"},{"name":"UCIBIO, Applied Molecular Biosciences Unit, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, 2819-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2086-5696","authenticated-orcid":false,"given":"Eul\u00e1lia","family":"Pereira","sequence":"additional","affiliation":[{"name":"LAQV\/REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, Rua Campo Alegre, 687, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Franco, R., Pedrosa, P., Carlos, F.F., Veigas, B., and Baptista, P.V. 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