{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T16:58:03Z","timestamp":1778605083492,"version":"3.51.4"},"reference-count":59,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T00:00:00Z","timestamp":1730851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"BioReset","award":["DivRestore\/0004\/2020"],"award-info":[{"award-number":["DivRestore\/0004\/2020"]}]},{"name":"BioReset","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"BioReset","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"BioReset","award":["SFRH\/BD\/115529\/2016"],"award-info":[{"award-number":["SFRH\/BD\/115529\/2016"]}]},{"name":"BioReset","award":["2022.10117.BD"],"award-info":[{"award-number":["2022.10117.BD"]}]},{"name":"FCT (Portuguese Foundation for the Science and Technology)","award":["DivRestore\/0004\/2020"],"award-info":[{"award-number":["DivRestore\/0004\/2020"]}]},{"name":"FCT (Portuguese Foundation for the Science and Technology)","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"FCT (Portuguese Foundation for the Science and Technology)","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"FCT (Portuguese Foundation for the Science and Technology)","award":["SFRH\/BD\/115529\/2016"],"award-info":[{"award-number":["SFRH\/BD\/115529\/2016"]}]},{"name":"FCT (Portuguese Foundation for the Science and Technology)","award":["2022.10117.BD"],"award-info":[{"award-number":["2022.10117.BD"]}]},{"name":"FCT PhD scholarship","award":["DivRestore\/0004\/2020"],"award-info":[{"award-number":["DivRestore\/0004\/2020"]}]},{"name":"FCT PhD scholarship","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}]},{"name":"FCT PhD scholarship","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}]},{"name":"FCT PhD scholarship","award":["SFRH\/BD\/115529\/2016"],"award-info":[{"award-number":["SFRH\/BD\/115529\/2016"]}]},{"name":"FCT PhD scholarship","award":["2022.10117.BD"],"award-info":[{"award-number":["2022.10117.BD"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>As a highly sensitive vibrational technique, Raman spectroscopy (RS) can provide valuable chemical and molecular data useful to characterise animal cell types, tissues and organs. As a label-free, rapid detection method, RS has been considered a valuable asset in forensics, biology and medicine. The technique has been applied to zebrafish for various purposes, including physiological, biochemical or bioaccumulation analyses. The available data point out its potential for the early diagnosis of detrimental effects elicited by toxicant exposure. Nevertheless, no baseline spectra are available for zebrafish embryos and larvae that could allow for suitable planning of toxicological assessments, comparison with toxicant-elicited spectra or mechanistic understanding of biochemical and physiological responses to the exposures. With this in mind, this work carried out a baseline characterisation of Raman spectra of zebrafish embryos and larvae throughout early development. Raman spectra were recorded from the iris, forebrain, melanocytes, heart, muscle and swim bladder between 24 and 168 h post-fertilisation. A chemometrics approach, based on partial least-squares discriminant analysis (PLS-DA), was used to obtain a Raman characterisation of each tissue or organ. In total, 117 Raman bands were identified, of which 24 were well represented and, thus, retained in the data analysed. Only three bands were found to be common to all organs and tissues. The PLS-DA provided a tentative Raman spectral fingerprint typical of each tissue or organ, reflecting the ongoing developmental dynamics. The bands showed frequencies previously assigned to collagen, cholesterol, various essential amino acids, carbohydrates and nucleic acids.<\/jats:p>","DOI":"10.3390\/bios14110538","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T11:13:37Z","timestamp":1730891617000},"page":"538","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6214-9324","authenticated-orcid":false,"given":"Isabel Oliveira","family":"Abreu","sequence":"first","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"},{"name":"ICBAS\u2014School of Medicine and Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3395-273X","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Teixeira","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6104-9834","authenticated-orcid":false,"given":"Rui","family":"Vilarinho","sequence":"additional","affiliation":[{"name":"IFIMUP\u2014Institute of Physics for Advanced Materials, Nanotechnology and Photonics, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6156-1141","authenticated-orcid":false,"given":"A. Cristina S.","family":"Rocha","sequence":"additional","affiliation":[{"name":"MARE\u2014Marine and Environmental Sciences Centre\/ARNET\u2014Aquatic Research Network, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4659-7503","authenticated-orcid":false,"given":"Joaquim Agostinho","family":"Moreira","sequence":"additional","affiliation":[{"name":"IFIMUP\u2014Institute of Physics for Advanced Materials, Nanotechnology and Photonics, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2584-1482","authenticated-orcid":false,"given":"Lu\u00eds","family":"Oliva-Teles","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3360-3783","authenticated-orcid":false,"given":"Laura","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1663-939X","authenticated-orcid":false,"given":"Ant\u00f3nio Paulo","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Av. General Norton de Matos, s\/n, 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences of the University of Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dantas, D., Soares, L., Novais, S., Vilarinho, R., Moreira, J.A., Silva, S., Fraz\u00e3o, O., Oliveira, T., Leal, N., and Fa\u00edsca, P. (2020). Discrimination of benign and malignant lesions in canine mammary tissue samples using Raman spectroscopy: A pilot study. Animals, 10.","DOI":"10.3390\/ani10091652"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"117102","DOI":"10.1016\/j.watres.2021.117102","article-title":"Raman spectroscopy applied to diatoms (microalgae, Bacillariophyta): Prospective use in the environmental diagnosis of freshwater ecosystems","volume":"198","author":"Pinto","year":"2021","journal-title":"Water Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1038\/nprot.2016.036","article-title":"Using Raman spectroscopy to characterize biological materials","volume":"11","author":"Butler","year":"2016","journal-title":"Nat. Protoc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"116623","DOI":"10.1016\/j.trac.2022.116623","article-title":"Trends in pharmaceutical analysis and quality control by modern Raman spectroscopic techniques","volume":"153","author":"Silge","year":"2022","journal-title":"TrAC-Trends Anal. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1002\/jcb.20884","article-title":"In vitro toxicology evaluation of pharmaceuticals using Raman micro-spectroscopy","volume":"99","author":"Owen","year":"2006","journal-title":"J. Cell. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1080\/05704928.2014.923902","article-title":"Raman spectroscopy of biological tissues","volume":"50","author":"Talari","year":"2015","journal-title":"Appl. Spectrosc. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1002\/jrs.6204","article-title":"Raman spectroscopy in cell biology and microbiology","volume":"52","author":"Pezzotti","year":"2021","journal-title":"J. Raman Spectrosc."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Meyer, T., Bergner, N., Bielecki, C., Krafft, C., Akimov, D., Romeike, B.F., Reichart, R., Kalff, R., Dietzek, B., and Popp, J. (2011). Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis. J. Biomed. Opt., 16.","DOI":"10.1117\/1.3533268"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.pdpdt.2013.01.008","article-title":"Advances in the clinical application of Raman spectroscopy for cancer diagnostics","volume":"10","author":"Kallaway","year":"2013","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"123904","DOI":"10.1016\/j.saa.2024.123904","article-title":"Rapid diagnosis of systemic lupus erythematosus by Raman spectroscopy combined with spiking neural network","volume":"310","author":"Chang","year":"2024","journal-title":"Spectrochim. Acta A"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"123905","DOI":"10.1016\/j.saa.2024.123905","article-title":"Early diagnosis of thyroid-associated ophthalmopathy using label-free Raman spectroscopy and multivariate analysis","volume":"310","author":"Wang","year":"2024","journal-title":"Spectrochim. Acta A"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Oliva-Teles, L., Pinto, R., Vilarinho, R., Carvalho, A.P., Moreira, J.A., and Guimar\u00e3es, L. (2022). Environmental diagnosis with Raman Spectroscopy applied to diatoms. Biosens. Bioelectron., 198.","DOI":"10.1016\/j.bios.2021.113800"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"L17","DOI":"10.1016\/j.bpj.2014.01.002","article-title":"Simultaneous Raman microspectroscopy and fluorescence imaging of bone mineralization in living zebrafish larvae","volume":"106","author":"Bennet","year":"2014","journal-title":"Biophys. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.bone.2015.02.020","article-title":"On the pathway of mineral deposition in larval zebrafish caudal fin bone","volume":"75","author":"Akiva","year":"2015","journal-title":"Bone"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6172","DOI":"10.1038\/s41467-020-19827-1","article-title":"In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy","volume":"11","author":"Horgan","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Andreana, M., Sturtzel, C., Spielvogel, C.P., Papp, L., Leitgeb, R., Drexler, W., Distel, M., and Unterhuber, A. (2021). Toward quantitative in vivo label-free tracking of lipid distribution in a zebrafish cancer model. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.675636"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1002\/bdrc.20210","article-title":"Developmental toxicity screening in zebrafish","volume":"93","author":"McCollum","year":"2011","journal-title":"Birth Defects Res. Part-C Embryo Today-Rev."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Rodrigues, P., Cunha, V., Ferreira, M., Reis-Henriques, M.A., Oliva-Teles, L., Guimar\u00e3es, L., and Carvalho, A.P. (2022). Differential molecular responses of zebrafish larvae to fluoxetine and norfluoxetine. Water, 14.","DOI":"10.3390\/w14030417"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"130909","DOI":"10.1016\/j.jhazmat.2023.130909","article-title":"Carbamazepine, venlafaxine, tramadol, and their main metabolites: Toxicological effects on zebrafish embryos and larvae","volume":"448","author":"Rodrigues","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Tao, Y., Du, C., Duan, B., Wang, W., Guo, H., Feng, J., Xu, H., and Li, Y. (2023). Eugenol exposure inhibits embryonic development and swim bladder formation in zebrafish. Comp. Biochem. Physiol. C-Toxicol. Pharmacol., 268.","DOI":"10.1016\/j.cbpc.2023.109602"},{"key":"ref_21","first-page":"42","article-title":"Specific nanotoxicity of graphene oxide during zebrafish embryogenesis","volume":"10","author":"Chen","year":"2015","journal-title":"Nanotoxicology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1002\/jat.3346","article-title":"Toxicity of single-wall carbon nanotubes functionalized with polyethylene glycol in zebrafish (Danio rerio) embryos","volume":"37","author":"Girardi","year":"2017","journal-title":"J. Appl. Toxicol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1093\/mutage\/gew050","article-title":"Diet-sourced carbon-based nanoparticles induce lipid alterations in tissues of zebrafish (Danio rerio) with genomic hypermethylation changes in brain","volume":"32","author":"Gorrochategui","year":"2017","journal-title":"Mutagenesis"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1016\/j.apsusc.2019.03.287","article-title":"Investigations on the in-vivo toxicity analysis of reduced graphene oxide\/TiO2 nanocomposite in zebrafish embryo and larvae (Danio rerio)","volume":"481","author":"Prakash","year":"2019","journal-title":"Appl. Surf. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"130860","DOI":"10.1016\/j.molstruc.2021.130860","article-title":"Synthesis, structural and spectroscopic characterization, in silico study, and antinociceptive effect in adult zebrafish of 2-(4-isobutylphenyl)-N\u2032-phenylpropanohydrazide","volume":"1243","author":"Garcia","year":"2021","journal-title":"J. Mol. Struct."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Lin, Y.C., Perevedentseva, E., Lin, Z.R., Chang, C.C., Chen, H.H., Yang, S.M., Lin, M.D., Karmenyan, A., Speranza, G., and Minati, L. (2022). Multimodal bioimaging using nanodiamond and gold hybrid nanoparticles. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-09317-3"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"14481","DOI":"10.1021\/jacs.6b09442","article-title":"Mineral formation in the larval zebrafish tail bone occurs via an acidic disordered calcium phosphate phase","volume":"138","author":"Akiva","year":"2016","journal-title":"J. Am. Chem. Soc."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"den Broeder, M.J., Moester, M.J.B., Kamstra, J.H., Cenijn, P.H., Davidoiu, V., Kamminga, L.M., Ariese, F., de Boer, J.F., and Legler, J. (2017). Altered adipogenesis in zebrafish larvae following high fat diet and chemical exposure is visualised by stimulated raman scattering microscopy. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18040894"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2687","DOI":"10.1039\/C4AN02227K","article-title":"Real-world carbon nanoparticle exposures induce brain and gonadal alterations in zebrafish (Danio rerio) as determined by biospectroscopy techniques","volume":"140","author":"Li","year":"2015","journal-title":"Analyst"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Chen, B., Zheng, Y.M., Zhang, M.Q., Han, Y., Zhang, J.P., and Hu, C.Q. (2020). Microarray expression profiling and Raman spectroscopy reveal anti-fatty liver action of berberine in a diet-induced larval zebrafish model. Front. Pharmacol., 10.","DOI":"10.3389\/fphar.2019.01504"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.envres.2014.06.017","article-title":"Evaluation of carbon nanotubes network toxicity in zebrafish (Danio rerio) model","volume":"134","author":"Filho","year":"2014","journal-title":"Environ. Res."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Yang, D.C., Tsay, H.J., Lin, S.Y., Chiou, S.H., Li, M.J., Chang, T.J., and Hung, S.C. (2008). cAMP\/PKA regulates osteogenesis, adipogenesis and ratio of RANKL\/OPG mRNA expression in mesenchymal stem cells by suppressing leptin. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0001540"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"125980","DOI":"10.1016\/j.jhazmat.2021.125980","article-title":"Microplastics as a vehicle of exposure to chemical contamination in freshwater systems: Current research status and way forward","volume":"417","author":"Eder","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Pinto, R., Vilarinho, R., Carvalho, A.P., Moreira, J.A., Guimar\u00e3es, L., and Oliva-Teles, L. (2022). Novel approach to freshwater diatom profiling and identification using raman spectroscopy and chemometric analysis. Water, 14.","DOI":"10.3390\/w14132116"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Hamada, K., Fujita, K., Smith, N.I., Kobayashi, M., Inouye, Y., and Kawata, S. (2008). Raman microscopy for dynamic molecular imaging of living cells. J. Biomed. Opt., 13.","DOI":"10.1117\/1.2952192"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.vibspec.2011.01.005","article-title":"Raman spectroscopic investigation on the microenvironment of the liver tissues of zebrafish (Danio rerio) due to titanium dioxide exposure","volume":"56","author":"Palaniappan","year":"2011","journal-title":"Vib. Spectrosc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1002\/jrs.4607","article-title":"Raman spectroscopy of lipids: A review","volume":"46","author":"Czamara","year":"2015","journal-title":"J. Raman Spectrosc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1364\/AOP.9.000315","article-title":"Raman spectroscopy: Techniques and applications in the life sciences","volume":"9","author":"Shipp","year":"2017","journal-title":"Adv. Opt. Photonics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.taap.2017.04.025","article-title":"Structure-toxicity relationship of cefoperazone and its impurities to developing zebrafish by transcriptome and Raman analysis","volume":"327","author":"Han","year":"2017","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"108803","DOI":"10.1016\/j.fsi.2023.108803","article-title":"Combined effects of high-fat diet and polystyrene microplastic exposure on microplastic bioaccumulation and lipid metabolism in zebrafish","volume":"137","author":"Du","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/etc.5767","article-title":"Toxicity of polystyrene nanoplastics in the liver and intestine of normal and high-fat-diet juvenile zebrafish","volume":"43","author":"Du","year":"2024","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1039\/C7EN00385D","article-title":"Ecotoxicological assessment of nanoparticle-containing acrylic copolymer dispersions in fairy shrimp and zebrafish embryos","volume":"4","author":"Galloway","year":"2017","journal-title":"Environ. Sci.-Nano"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1073\/pnas.79.5.1511","article-title":"Resonance Raman detection of structural dynamics at the active site in hemoglobin","volume":"79","author":"Ondrias","year":"1982","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0022-2836(80)90374-5","article-title":"Quaternary structures and low frequency molecular vibrations of haems of deoxy and oxyhaemoglobin studied by resonance Raman scattering","volume":"136","author":"Nagai","year":"1980","journal-title":"J. Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Kuhar, N., Sil, S., and Umapathy, S. (2021). Potential of Raman spectroscopic techniques to study proteins. Spectrochim. Acta Part A-Mol. Biomol. Spectrosc., 258.","DOI":"10.1016\/j.saa.2021.119712"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"100073","DOI":"10.1016\/j.giant.2021.100073","article-title":"Nanocomposite of Au and black phosphorus quantum dots as versatile probes for amphibious SERS spectroscopy, 3D photoacoustic imaging and cancer therapy","volume":"8","author":"Chen","year":"2021","journal-title":"Giant"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3863","DOI":"10.1021\/ac404051f","article-title":"Characterization of the pharmaceutical effect of drugs on atherosclerotic lesions in vivo using integrated fluorescence imaging and Raman spectral measurements","volume":"86","author":"Yang","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2448","DOI":"10.1038\/nprot.2006.255","article-title":"Measuring the optokinetic response of zebrafish larvae","volume":"1","author":"Brockerhoff","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Cohen, A., Popowitz, J., Delbridge-Perry, M., Rowe, C.J., and Connaughton, V.P. (2022). The role of estrogen and thyroid hormones in zebrafish visual system function. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.837687"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1039\/C9AN01588D","article-title":"Fourier transform infrared spectroscopy of developing bone mineral: From amorphous precursor to mature crystal","volume":"145","author":"Querido","year":"2020","journal-title":"Analyst"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"109879","DOI":"10.1016\/j.optlastec.2023.109879","article-title":"Highly sensitive fiber optic enhanced Raman scattering sensor","volume":"168","author":"Du","year":"2024","journal-title":"Opt. Laser Technol."},{"key":"ref_52","unstructured":"Milne, E.T. (1996). The Design and Development of Ultrasmall Microelectrode Probes for Surface Enhanced Raman Spectroscopy in Biological Media. [Ph.D. Thesis, The University of Michigan]."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2630","DOI":"10.1063\/1.457957","article-title":"Theoretical investigation of model polymers for eumelanins. I. Finite and infinite polymers","volume":"92","author":"Caldas","year":"1990","journal-title":"J. Chem. Phys."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.molstruc.2007.03.026","article-title":"Surface enhanced Raman scattering (SERS) and FTIR characterization of the sepia melanin pigment used in works of art","volume":"873","author":"Centeno","year":"2008","journal-title":"J. Mol. Struct."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Olmos, V., Marro, M., Loza-Alvarez, P., Rald\u00faa, D., Prats, E., Padr\u00f3s, F., Pi\u00f1a, B., Tauler, R., and de Juan, A. (2018). Combining hyperspectral imaging and chemometrics to assess and interpret the effects of environmental stressors on zebrafish eye images at tissue level. J. Biophotonics, 11.","DOI":"10.1002\/jbio.201700089"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Markin, P.A., Brito, A., Moskaleva, N.E., Tagliaro, F., Tarasov, V.V., La Frano, M.R., Savitskii, M.V., and Appolonova, S.A. (2021). Short- and medium-term exposures of diazepam induce metabolomic alterations associated with the serotonergic, dopaminergic, adrenergic and aspartic acid neurotransmitter systems in zebrafish (Danio rerio) embryos\/larvae. Comp. Biochem. Physiol. Part D-Genom. Proteom., 38.","DOI":"10.1016\/j.cbd.2021.100816"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1042\/EBC20180038","article-title":"Understanding biochemistry: Structure and function of nucleic acids","volume":"63","author":"Minchin","year":"2019","journal-title":"Essays Biochem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.aquatox.2015.02.016","article-title":"Dioxin inhibition of swim bladder development in zebrafish: Is it secondary to heart failure?","volume":"162","author":"Yue","year":"2015","journal-title":"Aquat. Toxicol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"106180","DOI":"10.1016\/j.aquatox.2022.106180","article-title":"Cadmium impairs zebrafish swim bladder development via ROS mediated inhibition of the Wnt\/Hedgehog pathway","volume":"247","author":"Zhang","year":"2022","journal-title":"Aquat. 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