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While traditional diagnostic methods provide precise measurements of phytohormones such as salicylic acid (SA), a key regulator of plant defence responses, their reliance on bulky equipment and lengthy analysis times limits field applicability. This study presents a microfluidic-based aptamer assay for SA detection, enabling rapid and sensitive fluorescence-based readout from plant samples. A tailored sample pre-treatment protocol was developed and validated with real strawberry samples using HPLC measurements. The assay demonstrated a detection limit ranging from 10\u22129 to 10\u22126 mg\/mL, within the relevant range for early infection diagnosis. The integration of the microfluidic platform with the optimised pre-treatment protocol offers a portable, cost-effective solution for on-site phytohormone analysis, providing a valuable tool for early infection detection and improved crop management.<\/jats:p>","DOI":"10.3390\/bios15050266","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T20:11:48Z","timestamp":1745352708000},"page":"266","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in Botrytis cinerea-Infected Strawberries"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1000-1181","authenticated-orcid":false,"given":"Cristiana","family":"Domingues","sequence":"first","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2013Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, Portugal"}]},{"given":"Rafaela R.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2013Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7656-7950","authenticated-orcid":false,"given":"Rodolfo G.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2013Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, Portugal"}]},{"given":"Ana Margarida","family":"Fortes","sequence":"additional","affiliation":[{"name":"Instituto de Biossistemas e Ci\u00eancias Integrativas (BioISI), Faculdade de Ci\u00eancias de Lisboa, Universidade de Lisboa, 1749-016 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5306-4409","authenticated-orcid":false,"given":"Virginia","family":"Chu","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2013Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5677-3024","authenticated-orcid":false,"given":"Jo\u00e3o Pedro","family":"Conde","sequence":"additional","affiliation":[{"name":"Instituto de Engenharia de Sistemas e Computadores\u2013Microsistemas e Nanotecnologias (INESC-MN), Rua Alves Redol, 1000-029 Lisbon, Portugal"},{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, Avenida Rovisco Pais, 1049-001 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15401","DOI":"10.1021\/acs.jafc.4c00454","article-title":"Recent Advances in Microfluidics for the Early Detection of Plant Diseases in Vegetables, Fruits, and Grains Caused by Bacteria, Fungi, and Viruses","volume":"72","author":"Zhao","year":"2024","journal-title":"J. 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