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Urine-based metabolite biomarkers offer a non-invasive alternative for CRC triage, but translating a laboratory assay into a point-of-care (PoC) system requires standardized fluid handling, operator-independent timing, field-compatible reagents, and quantitative calibration. Here, we present a low-cost, semi-automated PoC platform integrating a validated, sequential, three-metabolite CRC-biomarker assay with robotic fluid handling, a motorized chromatographic module, an optical reader, Bluetooth electronics, and tablet-guided control. The platform measures urinary diacetylspermine and hippuric acid, with creatinine serving as a normalization reference, and reports absolute quantitative concentrations. Automated dilution, tube positioning, timed incubation, controlled column elution, and software-guided transfer reduce operator-dependent variation. Using pooled urine samples spiked at clinically relevant concentrations, the creatinine assay showed a strong quadratic response (0\u201350 mM, R2 = 0.998), as did the diacetylspermine assay (0\u20134 \u03bcM, R2 = 0.979), while the hippuric acid assay showed a linear response of R2 = 0.989. The RGB sensor tracked the concentration-dependent trend observed with a laboratory microplate reader (R2 = 0.968\u20130.999). Reagents reformulated as lyophilized or pre-weighed, vacuum-sealed kits withstood accelerated heat and humidity (45 \u00b0C\/70% RH) testing and international shipping to the pilot site very well. At approximately USD 490 for the complete instrument (including tablet) and roughly USD 7.65 in consumables per screening test, the platform offers a practical, quantitative, and portable route for decentralized CRC screening.<\/jats:p>","DOI":"10.3390\/bios16090485","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:56:01Z","timestamp":1788339361000},"page":"485","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Portable Colorimetric Biosensor Platform for Urinary Colorectal Cancer Biomarker Testing in Low-Resource Settings"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8414-1174","authenticated-orcid":false,"given":"Prashanthi","family":"Kovur","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, CW-405, Biological Sciences Building, University of Alberta, Edmonton, AB T6G 2E9, 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cancer","volume":"375","author":"Crosby","year":"2022","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Falzone, L., Salomone, S., and Libra, M. (2018). Evolution of cancer pharmacological treatments at the turn of the third millennium. Front. Pharmacol., 9.","DOI":"10.3389\/fphar.2018.01300"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ramola, A., Shakya, A.K., and Bergman, A. (2025). Comprehensive Analysis of Advancement in Optical Biosensing Techniques for Early Detection of Cancerous Cells. Biosensors, 15.","DOI":"10.3390\/bios15050292"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2992","DOI":"10.1002\/ijc.32055","article-title":"Global trends in colorectal cancer mortality: Projections to the year 2035","volume":"144","author":"Araghi","year":"2019","journal-title":"Int. J. Cancer"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1136\/gutjnl-2015-310912","article-title":"Global patterns and trends in colorectal cancer incidence and mortality","volume":"66","author":"Arnold","year":"2017","journal-title":"Gut"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1136\/gutjnl-2022-327736","article-title":"Global burden of colorectal cancer in 2020 and 2040: Incidence and mortality estimates from GLOBOCAN","volume":"72","author":"Morgan","year":"2023","journal-title":"Gut"},{"key":"ref_7","unstructured":"Cancer Research UK (2024). Survival for Bowel Cancer, Cancer Research UK."},{"key":"ref_8","unstructured":"NHS England (2023). Cancer Survival in England, Cancers Diagnosed 2016 to 2020, Followed Up to 2021, NHS England."},{"key":"ref_9","first-page":"646","article-title":"The Present and Future of Colorectal Cancer Screening","volume":"18","author":"Chung","year":"2022","journal-title":"Gastroenterol. Hepatol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Zygulska, A.L., and Pierzchalski, P. (2022). Novel Diagnostic Biomarkers in Colorectal Cancer. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23020852"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Carneiro, M.C.C.G., Rodrigues, L.R., Moreira, F.T.C., and Sales, M.G.F. (2022). Colorimetric Paper-Based Sensors against Cancer Biomarkers. Sensors, 22.","DOI":"10.3390\/s22093221"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"G\u00f3mez-Molina, R., Su\u00e1rez, M., Mart\u00ednez, R., Chilet, M., Bau\u00e7a, J.M., and Mateo, J. (2024). Utility of Stool-Based Tests for Colorectal Cancer Detection: A Comprehensive Review. Healthcare, 12.","DOI":"10.3390\/healthcare12161645"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"171","DOI":"10.7326\/M13-1484","article-title":"Accuracy of Fecal Immunochemical Tests for Colorectal Cancer","volume":"160","author":"Lee","year":"2014","journal-title":"Ann. Intern. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1177\/10105395251387224","article-title":"An Evaluation of the Effectiveness of Patient Navigation on Colonoscopy Screening Adherence for Colorectal Cancer: A Randomized Controlled Trial","volume":"37","author":"See","year":"2025","journal-title":"Asia Pac. J. Public Health"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"79","DOI":"10.34172\/biomedrb.2024.13","article-title":"Novel Electrochemical Biosensors for Detecting Specific Colorectal Cancer Biomarkers, Delving Into Advancements in Early Detection and Personalized Treatment Strategies","volume":"2","author":"Dong","year":"2024","journal-title":"Biomed. Res. Bull."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"119715","DOI":"10.1016\/j.cca.2024.119715","article-title":"Nucleic acid-based electrochemical biosensors","volume":"559","author":"Gunasekaran","year":"2024","journal-title":"Clin. Chim. Acta"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Li, H., Liang, Z., Meng, Q., Han, J., Yin, Z., Xu, Z., Li, X., Chen, J., and Wang, L. (2026). A Point-of-Care High-throughput Biosensing System of Home Monitoring for CRC Postoperative Recurrence by Detecting CEA, MicroRNA-21, and IL-6. IEEE Trans. Biomed. Eng., 1\u20138.","DOI":"10.1109\/TBME.2026.3654630"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2475581","DOI":"10.1080\/15384047.2025.2475581","article-title":"Electrochemical biosensing in oncology: A review advancements and prospects for cancer diagnosis","volume":"26","author":"Noreen","year":"2025","journal-title":"Cancer Biol. Ther."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Myers, F.B., Moffatt, B., El Khaja, R., Chatterjee, T., Marwaha, G., McGee, M., and Mitra, D. (2022). A robust, low-cost instrument for real-time colorimetric isothermal nucleic acid amplification. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0256789"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nguyen, T.K., Jun, H., Louis, J.M., Mazigo, E., Lee, W.J., Youm, H.C., Shin, J., Lungu, D.K., Kanyemba, C., and Ahmed, M.A. (2024). Enhancing malaria detection in resource-limited areas: A high-performance colorimetric LAMP assay for Plasmodium falciparum screening. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0298087"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Yilmaz, A., Gencoglan, G., Varol, R., Demircali, A.A., Keshavarz, M., and Uvet, H. (2022). MobileSkin: Classification of Skin Lesion Images Acquired Using Mobile Phone-Attached Hand-Held Dermoscopes. J. Clin. Med., 11.","DOI":"10.3390\/jcm11175102"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e2300617","DOI":"10.1002\/smll.202300617","article-title":"Deep Learning-Enabled Multiplexed Point-of-Care Sensor using a Paper-Based Fluorescence Vertical Flow Assay","volume":"19","author":"Goncharov","year":"2023","journal-title":"Small"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"eaax4473","DOI":"10.1126\/sciadv.aax4473","article-title":"Point-of-care biomarker quantification enabled by sample-specific calibration","volume":"5","author":"Mcnerney","year":"2019","journal-title":"Sci. Adv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"14881","DOI":"10.1021\/acs.chemrev.1c01012","article-title":"Toward Next Generation Lateral Flow Assays: Integration of Nanomaterials","volume":"122","author":"Parolo","year":"2022","journal-title":"Chem. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3233\/CBM-220034","article-title":"Identification of urinary biomarkers of colorectal cancer: Towards the development of a colorectal screening test in limited resource settings","volume":"36","author":"Zhang","year":"2023","journal-title":"Cancer Biomark."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2500062","DOI":"10.1200\/GO-25-00062","article-title":"Point-of-Care Urine Metabolomics Test to Diagnose Colorectal Cancers in Low- and Middle-Income Countries: A Pilot Controlled Trial in Southwestern Nigeria","volume":"11","author":"Adeomi","year":"2025","journal-title":"JCO Glob. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"116186","DOI":"10.1016\/j.bios.2024.116186","article-title":"Development of a point-of-care colorimetric metabolomic sensor platform","volume":"253","author":"Bai","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"115717","DOI":"10.1016\/j.ab.2024.115717","article-title":"A novel colorimetric assay for the detection of urinary N1, N12-diacetylspermine, a known biomarker for colorectal cancer","volume":"697","author":"Bhattacharyya","year":"2025","journal-title":"Anal. Biochem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"115303","DOI":"10.1016\/j.ab.2023.115303","article-title":"A fast and accurate colorimetric assay for quantifying hippuric acid in human urine","volume":"680","author":"Bhattacharyya","year":"2023","journal-title":"Anal. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"818","DOI":"10.5694\/j.1326-5377.1936.tb103256.x","article-title":"The Colorimetric Determination of Creatinine in Urine and Blood with 3,5-Dinitrobenzoic ACID","volume":"2","author":"Bolliger","year":"1936","journal-title":"Med. J. Aust."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.talanta.2018.12.003","article-title":"An alternative, single-point method for creatinine determination in urine samples with optoelectronic detector. Critical comparison to Jaff\u00e9 method","volume":"195","author":"Tymecki","year":"2019","journal-title":"Talanta"}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/16\/9\/485\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:00:14Z","timestamp":1788778814000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/16\/9\/485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":31,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["bios16090485"],"URL":"https:\/\/doi.org\/10.3390\/bios16090485","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]}}}