{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T10:37:38Z","timestamp":1775039858239,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T00:00:00Z","timestamp":1475020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["SFRH\/BPD\/79566\/2011"],"award-info":[{"award-number":["SFRH\/BPD\/79566\/2011"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["PD\/BD\/109687\/2015"],"award-info":[{"award-number":["PD\/BD\/109687\/2015"]}]},{"name":"UCIBIO-REQUIMTE","award":["Pest-C\/EQB\/LA0006\/2013"],"award-info":[{"award-number":["Pest-C\/EQB\/LA0006\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>From the bench-mark work on microfluidics from the Whitesides\u2019s group in 2007, paper technology has experienced significant growth, particularly regarding applications in biomedical research and clinical diagnostics. Besides the structural properties supporting microfluidics, other advantageous features of paper materials, including their versatility, disposability and low cost, show off the great potential for the development of advanced and eco-friendly analytical tools. Consequently, paper was quickly employed in the field of electrochemical sensors, being an ideal material for producing custom, tailored and miniaturized devices. Stencil-, inkjet-, or screen-printing are the preferential techniques for electrode manufacturing. Not surprisingly, we witnessed a rapid increase in the number of publications on paper based screen-printed sensors at the turn of the past decade. Among the sensing strategies, various biosensors, coupling electrochemical detectors with biomolecules, have been proposed. This work provides a critical review and a discussion on the future progress of paper technology in the context of miniaturized printed electrochemical biosensors.<\/jats:p>","DOI":"10.3390\/bios6040051","type":"journal-article","created":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T10:56:13Z","timestamp":1475060173000},"page":"51","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Biosensing with Paper-Based Miniaturized Printed Electrodes\u2013A Modern Trend"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4821-2358","authenticated-orcid":false,"given":"C\u00e9lia","family":"Silveira","sequence":"first","affiliation":[{"name":"UCIBIO, REQUIMTE, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade NOVA de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0414-5242","authenticated-orcid":false,"given":"Tiago","family":"Monteiro","sequence":"additional","affiliation":[{"name":"UCIBIO, REQUIMTE, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4508-7379","authenticated-orcid":false,"given":"Maria","family":"Almeida","sequence":"additional","affiliation":[{"name":"UCIBIO, REQUIMTE, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Monte de Caparica, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz (CiiEM), Instituto Superior de Ci\u00eancias da Sa\u00fade Egas Moniz, Campus Universit\u00e1rio, Quinta da Granja, 2829-511 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2016,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1039\/c3lc50169h","article-title":"Paper-based microfluidic point-of-care diagnostic devices","volume":"13","author":"Yetisen","year":"2013","journal-title":"Lab Chip"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1007\/s00216-015-9249-2","article-title":"The future point-of-care detection of disease and its data capture and handling","volume":"408","author":"Gamarra","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.plabm.2015.12.002","article-title":"Practical challenges related to point of care testing","volume":"4","author":"Shaw","year":"2016","journal-title":"Pract. Lab. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Pai, N.P., Vadnais, C., Denkinger, C., Engel, N., and Pai, M. (2012). Point-of-Care Testing for Infectious Diseases: Diversity, Complexity, and Barriers in Low-And Middle-Income Countries. PLoS Med., 9.","DOI":"10.1371\/journal.pmed.1001306"},{"key":"ref_5","first-page":"75","article-title":"Platform Technologies for Molecular Diagnostics Near the Patient\u2019s Bedside","volume":"133","author":"Schumacher","year":"2013","journal-title":"Adv. Biochem. Eng. Biotechnol."},{"key":"ref_6","first-page":"155","article-title":"Existing and Emerging Technologies for Point-of-Care Testing","volume":"35","author":"Price","year":"2014","journal-title":"Clin. Biochem. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"914","DOI":"10.2471\/BLT.12.102780","article-title":"Low-cost tools for diagnosing and monitoring HIV infection in low-resource settings","volume":"90","author":"Wu","year":"2012","journal-title":"Bull. World Health Organ."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4750","DOI":"10.1039\/C4AN01003E","article-title":"Enabling robust quantitative readout in an equipment-free model of device development","volume":"139","author":"Fu","year":"2014","journal-title":"Analyst"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3184","DOI":"10.1039\/c3cs35528d","article-title":"Biosensors: Sense and sensibility","volume":"42","author":"Turner","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1038\/nbt0806-891","article-title":"Moving diagnostics from the bench to the bedside","volume":"24","author":"Kling","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.snb.2013.11.054","article-title":"A \u201cgreen\u201d cellulose paper based glucose amperometric biosensor","volume":"193","author":"Lawrence","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.bios.2013.10.075","article-title":"Advances in paper-based point-of-care diagnostics","volume":"54","author":"Hu","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1002\/anie.200603817","article-title":"Patterned paper as a platform for inexpensive, low-volume, portable bioassays","volume":"46","author":"Martinez","year":"2007","journal-title":"Angew. Chemie. Int. Ed."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1021\/ac503968p","article-title":"Recent Developments in Paper-Based Microfluidic Devices","volume":"87","author":"Cate","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1039\/C2CS35255A","article-title":"Paper-based nanobiosensors for diagnostics","volume":"42","author":"Parolo","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7573","DOI":"10.1007\/s00216-013-6911-4","article-title":"Sensing approaches on paper-based devices: A review","volume":"405","author":"Nery","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7091","DOI":"10.1021\/ac901071p","article-title":"Understanding Wax Printing: A Simple Micropatterning Process for Paper-Based Microfluidics","volume":"81","author":"Carrilho","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11505","DOI":"10.3390\/s120911505","article-title":"Recent advances in paper-based sensors","volume":"12","author":"Liana","year":"2012","journal-title":"Sensors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1002\/elan.201400036","article-title":"Paper-Based Electrochemical Biosensors: From Test Strips to Paper-Based Microfluidics","volume":"26","author":"Liu","year":"2014","journal-title":"Electroanalysis"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3423","DOI":"10.1007\/s00216-013-6813-5","article-title":"Amperometric sensing\u2014Bioelectroanalysis","volume":"405","author":"Seeber","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3619","DOI":"10.1007\/s00216-013-6786-4","article-title":"Small electron-transfer proteins as mediators in enzymatic electrochemical biosensors","volume":"405","author":"Silveira","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1039\/b916888e","article-title":"Advanced printing and deposition methodologies for the fabrication of biosensors and biodevices","volume":"135","author":"Morrin","year":"2010","journal-title":"Analyst"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1039\/B917150A","article-title":"Electrochemical sensing in paper-based microfluidic devices","volume":"10","author":"Nie","year":"2010","journal-title":"Lab Chip"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5821","DOI":"10.1021\/ac9007573","article-title":"Electrochemical detection for paper-based microfluidics","volume":"81","author":"Dungchai","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.elecom.2012.03.041","article-title":"Application of conducting paper for selective detection of troponin","volume":"20","author":"Jagadeesan","year":"2012","journal-title":"Electrochem. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"10071","DOI":"10.1021\/ac302537r","article-title":"Paper-based enzyme immobilization for flow injection electrochemical biosensor integrated with reagent-loaded cartridge toward portable modular device","volume":"84","author":"Tan","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.bios.2012.01.017","article-title":"Paper supported immunosensor for detection of antibiotics","volume":"33","author":"Wu","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.snb.2012.10.113","article-title":"A low-cost paper-based inkjet-printed platform for electrochemical analyses","volume":"177","author":"Vanamo","year":"2013","journal-title":"Sens. Actuators B Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.aca.2013.06.021","article-title":"Electrochemical detection of glucose from whole blood using paper-based microfluidic devices","volume":"788","author":"Noiphung","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.snb.2012.11.002","article-title":"A new approach for paper-based analytical devices with electrochemical detection based on graphite pencil electrodes","volume":"177","author":"Santhiago","year":"2013","journal-title":"Sens. Actuators B Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"54402","DOI":"10.1088\/1468-6996\/14\/5\/054402","article-title":"A microfluidic paper-based electrochemical biosensor array for multiplexed detection of metabolic biomarkers","volume":"14","author":"Zhao","year":"2013","journal-title":"Sci. Technol. Adv. Mater."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.bios.2013.12.067","article-title":"A paper disk equipped with graphene\/polyaniline\/Au nanoparticles\/glucose oxidase biocomposite modified screen-printed electrode: Toward whole blood glucose determination","volume":"56","author":"Kong","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.aca.2014.01.024","article-title":"Graphene nano-ink biosensor arrays on a microfluidic paper for multiplexed detection of metabolites","volume":"813","author":"Labroo","year":"2014","journal-title":"Anal. Chim. Acta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.bios.2014.05.012","article-title":"Growth of gold-manganese oxide nanostructures on a 3D origami device for glucose-oxidase label based electrochemical immunosensor","volume":"61","author":"Li","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.bios.2013.08.018","article-title":"Novel paper-based cholesterol biosensor using graphene\/polyvinylpyrrolidone\/polyaniline nanocomposite","volume":"52","author":"Ruecha","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.bios.2013.08.039","article-title":"A paper-based microfluidic electrochemical immunodevice integrated with amplification-by-polymerization for the ultrasensitive multiplexed detection of cancer biomarkers","volume":"52","author":"Wu","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.snb.2014.06.077","article-title":"Paper-fluidic electrochemical biosensing platform with enzyme paper and enzymeless electrodes","volume":"203","author":"Yang","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.talanta.2015.05.008","article-title":"Electrochemical K-562 cells sensor based on origami paper device for point-of-care testing","volume":"145","author":"Ge","year":"2015","journal-title":"Talanta"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.snb.2015.06.009","article-title":"Ultrasensitive electrochemical cancer cells sensor based on trimetallic dendritic Au@PtPd nanoparticles for signal amplification on lab-on-paper device","volume":"220","author":"Ge","year":"2015","journal-title":"Sens. Actuators B Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9009","DOI":"10.1021\/acs.analchem.5b02210","article-title":"Detection of Hepatitis B Virus DNA with a Paper Electrochemical Sensor","volume":"87","author":"Li","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.aca.2015.08.007","article-title":"A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection","volume":"891","author":"Nantaphol","year":"2015","journal-title":"Anal. Chim. Acta"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.talanta.2014.10.061","article-title":"Amperometric measurements of ethanol on paper with a glucometer","volume":"134","author":"Wu","year":"2015","journal-title":"Talanta"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.bios.2015.12.020","article-title":"A 3D paper-based enzymatic fuel cell for self-powered, low-cost glucose monitoring","volume":"79","author":"Fischer","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.snb.2016.03.031","article-title":"Fully-drawn origami paper analytical device for electrochemical detection of glucose","volume":"231","author":"Li","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"94006","DOI":"10.1088\/0957-4484\/25\/9\/094006","article-title":"A low cost, safe, disposable, rapid and self-sustainable paper-based platform for diagnostic testing: Lab-on-paper","volume":"25","author":"Costa","year":"2014","journal-title":"Nanotechnology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"9131","DOI":"10.1021\/ac801729t","article-title":"Paper-Based Microfluidic Devices by Plasma Treatment","volume":"80","author":"Li","year":"2008","journal-title":"Anal. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.colsurfb.2009.12.023","article-title":"Fabrication of paper-based microfluidic sensors by printing","volume":"76","author":"Li","year":"2010","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1039\/C0AN00406E","article-title":"A low-cost, simple, and rapid fabrication method for paper-based microfluidics using wax screen-printing","volume":"136","author":"Dungchai","year":"2011","journal-title":"Analyst"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1039\/b714449k","article-title":"Electrochemical biosensors","volume":"39","author":"Ronkainen","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2012.10.060","article-title":"Sandwich-type immunosensors and immunoassays exploiting nanostructure labels: A review","volume":"758","author":"Pei","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2538","DOI":"10.1039\/C5LC00235D","article-title":"Inkjet printing for biosensor fabrication: Combining chemistry and technology for advanced manufacturing","volume":"15","author":"Li","year":"2015","journal-title":"Lab Chip"}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/6\/4\/51\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:32:00Z","timestamp":1760211120000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/6\/4\/51"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,28]]},"references-count":51,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2016,12]]}},"alternative-id":["bios6040051"],"URL":"https:\/\/doi.org\/10.3390\/bios6040051","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,28]]}}}