{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T17:54:00Z","timestamp":1770918840535,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T00:00:00Z","timestamp":1585094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-29030"],"award-info":[{"award-number":["POCI-01-0145-FEDER-29030"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/136007\/2018"],"award-info":[{"award-number":["SFRH\/BD\/136007\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/132751\/2017"],"award-info":[{"award-number":["SFRH\/BD\/132751\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European COST Action EPITRAN","award":["CA16120"],"award-info":[{"award-number":["CA16120"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>RNA methylation at position N6 in adenosine (m6A) and its associated methyltransferase complex (MTC) are involved in tumorigenesis. We aimed to explore m6A biological function for long non-coding RNAs (lncRNAs) in prostate cancer (PCa) and its clinical significance. m6A and MTC levels in PCa cells were characterized by ELISA and western blot. Putative m6A-regulated lncRNAs were identified and validated by lncRNA profiler qPCR array and bioinformatics analysis, followed by m6A\/RNA co-immunoprecipitation. Impact of m6A depletion on RNA stability was assessed by Actinomycin D assay. The association of m6A-levels with PCa prognosis was examined in clinical samples. Higher m6A-levels and VIRMA overexpression were detected in metastatic castration-resistant PCa (mCRPC) cells (p &lt; 0.05). VIRMA knockdown in PC-3 cells significantly decreased m6A-levels (p = 0.0317), attenuated malignant phenotype and suppressed the expression of oncogenic lncRNAs CCAT1 and CCAT2 (p &lt; 0.00001). VIRMA depletion and m6A reduction decreased the stability and abundance of CCAT1\/2 transcripts. Higher expression of VIRMA, CCAT1, and CCAT2 as a group variable was an independent predictor of poor prognosis (HR = 9.083, CI95% 1.911\u201343.183, p = 0.006). VIRMA is a critical factor sustaining m6A-levels in PCa cells. VIRMA downregulation attenuates the aggressive phenotype of PCa by overall reduction of m6A-levels decreasing stability and abundance of oncogenic lncRNAs.<\/jats:p>","DOI":"10.3390\/cancers12040771","type":"journal-article","created":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T13:10:47Z","timestamp":1585141847000},"page":"771","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["VIRMA-Dependent N6-Methyladenosine Modifications Regulate the Expression of Long Non-Coding RNAs CCAT1 and CCAT2 in Prostate Cancer"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6746-6807","authenticated-orcid":false,"given":"Daniela","family":"Barros-Silva","sequence":"first","affiliation":[{"name":"Cancer Biology and Epigenetics Group, Research Center of Portuguese Oncology Institute of Porto (GEBC CI-IPOP) and Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6829-1391","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Lobo","sequence":"additional","affiliation":[{"name":"Cancer Biology and Epigenetics Group, Research Center of Portuguese Oncology Institute of Porto (GEBC CI-IPOP) and Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200072 Porto, Portugal"},{"name":"Department of Pathology, Portuguese Oncology Institute of Porto (IPOP), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2212-3872","authenticated-orcid":false,"given":"Catarina","family":"Guimar\u00e3es-Teixeira","sequence":"additional","affiliation":[{"name":"Cancer Biology and Epigenetics Group, Research Center of Portuguese Oncology Institute of Porto (GEBC CI-IPOP) and Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200072 Porto, Portugal"}]},{"given":"Isa","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Cancer Biology and Epigenetics Group, Research Center of Portuguese Oncology Institute of Porto (GEBC CI-IPOP) and Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200072 Porto, Portugal"},{"name":"Department of Pathology, Portuguese Oncology Institute of Porto (IPOP), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7838-7493","authenticated-orcid":false,"given":"Jorge","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Urology, Portuguese Oncology Institute of Porto (IPOP), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"}]},{"given":"Elena S.","family":"Martens-Uzunova","sequence":"additional","affiliation":[{"name":"Department of Urology, Cancer Institute, Erasmus MC University Medical Center Rotterdam, Be-432A, PO Box 2040, 3000 CA Rotterdam, The Netherlands"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3171-4666","authenticated-orcid":false,"given":"Rui","family":"Henrique","sequence":"additional","affiliation":[{"name":"Cancer Biology and Epigenetics Group, Research Center of Portuguese Oncology Institute of Porto (GEBC CI-IPOP) and Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200072 Porto, Portugal"},{"name":"Department of Pathology, Portuguese Oncology Institute of Porto (IPOP), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"},{"name":"Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar, University of Porto (ICBAS-UP), Rua Jorge Viterbo Ferreira 228, 4050-513 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4186-5345","authenticated-orcid":false,"given":"Carmen","family":"Jer\u00f3nimo","sequence":"additional","affiliation":[{"name":"Cancer Biology and Epigenetics Group, Research Center of Portuguese Oncology Institute of Porto (GEBC CI-IPOP) and Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200072 Porto, Portugal"},{"name":"Department of Pathology, Portuguese Oncology Institute of Porto (IPOP), R. Dr. Ant\u00f3nio Bernardino de Almeida, 4200-072 Porto, Portugal"},{"name":"Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar, University of Porto (ICBAS-UP), Rua Jorge Viterbo Ferreira 228, 4050-513 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.14740\/wjon1191","article-title":"Epidemiology of Prostate Cancer","volume":"10","author":"Rawla","year":"2019","journal-title":"World J. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1158\/2159-8290.CD-16-1292","article-title":"The epitranscriptome of noncoding RNAs in cancer","volume":"7","author":"Esteller","year":"2017","journal-title":"Cancer Discov."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1146\/annurev-cellbio-100616-060758","article-title":"Rethinking m6A readers, writers, and erasers","volume":"33","author":"Meyer","year":"2017","journal-title":"Ann. Rev. Cell Dev. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/s11684-018-0654-8","article-title":"RNA m 6 A modification and its function in diseases","volume":"12","author":"Tong","year":"2018","journal-title":"Front. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.molcel.2019.04.025","article-title":"Where, when, and how: Context-dependent functions of RNA methylation writers, readers, and erasers","volume":"74","author":"Shi","year":"2019","journal-title":"Mol. Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1038\/s41422-018-0040-8","article-title":"Dynamic transcriptomic m 6 A decoration: Writers, erasers, readers and functions in RNA metabolism","volume":"28","author":"Yang","year":"2018","journal-title":"Cell Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1038\/s41421-018-0019-0","article-title":"VIRMA mediates preferential m 6 A mRNA methylation in 3\u2032 UTR and near stop codon and associates with alternative polyadenylation","volume":"4","author":"Yue","year":"2018","journal-title":"Cell Discov."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.gpb.2018.03.001","article-title":"Structural insights into N6-methyladenosine (m6A) modification in the transcriptome","volume":"16","author":"Huang","year":"2018","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.pharmthera.2018.04.011","article-title":"Mechanism of N6-methyladenosine modification and its emerging role in cancer","volume":"189","author":"Zhang","year":"2018","journal-title":"Pharmacol. Ther."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.ccell.2017.02.013","article-title":"m6A demethylase ALKBH5 maintains tumorigenicity of glioblastoma stem-like cells by sustaining FOXM1 expression and cell proliferation program","volume":"31","author":"Zhang","year":"2017","journal-title":"Cancer Cell"},{"key":"ref_11","first-page":"621","article-title":"The epitranscriptome m6A writer METTL3 promotes chemo-and radioresistance in pancreatic cancer cells","volume":"52","author":"Taketo","year":"2018","journal-title":"Int. J. Oncol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1002\/mc.22782","article-title":"FTO regulates the chemo-radiotherapy resistance of cervical squamous cell carcinoma (CSCC) by targeting \u03b2-catenin through mRNA demethylation","volume":"57","author":"Zhou","year":"2018","journal-title":"Mol. Carcinog."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.bbagrm.2015.10.019","article-title":"The emerging epitranscriptomics of long noncoding RNAs","volume":"1859","author":"Shafik","year":"2016","journal-title":"Biochimica Biophysica Acta"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Jacob, R., Zander, S., and Gutschner, T. (2017). The dark side of the epitranscriptome: Chemical modifications in long non-coding RNAs. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18112387"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.euf.2016.11.012","article-title":"The molecular evolution of castration-resistant prostate cancer","volume":"2","author":"Ceder","year":"2016","journal-title":"Eur. Urol. Focus"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"W98","DOI":"10.1093\/nar\/gkx247","article-title":"GEPIA: A web server for cancer and normal gene expression profiling and interactive analyses","volume":"45","author":"Tang","year":"2017","journal-title":"Nucl. Acids Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"116","DOI":"10.3389\/fcell.2019.00116","article-title":"Interplay between N6-methyladenosine (m6A) and non-coding RNAs in cell development and cancer","volume":"7","author":"Fazi","year":"2019","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1016\/j.eururo.2013.12.003","article-title":"Long noncoding RNA in prostate, bladder, and kidney cancer","volume":"65","author":"Croce","year":"2014","journal-title":"Eur. Urol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e91","DOI":"10.1093\/nar\/gkw104","article-title":"SRAMP: Prediction of mammalian N6-methyladenosine (m6A) sites based on sequence-derived features","volume":"44","author":"Zhou","year":"2016","journal-title":"Nucl. Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"14581","DOI":"10.1002\/jcp.28176","article-title":"Colon Cancer-Associated Transcripts 1 and 2: Roles and functions in human cancers","volume":"234","author":"Taheri","year":"2019","journal-title":"J. Cell. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"794","DOI":"10.3934\/biophy.2015.4.794","article-title":"The interplay of long non-coding RNAs and MYC in cancer","volume":"2","author":"Hamilton","year":"2015","journal-title":"AIMS Biophys."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ajur.2017.11.004","article-title":"Updates in prostate cancer detections and treatments\u2013Messages from 2017 EAU and AUA","volume":"5","author":"Xie","year":"2018","journal-title":"Asian J. Urol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.2147\/OTT.S180954","article-title":"Kiaa1429 regulates the migration and invasion of hepatocellular carcinoma by altering m6a modification of ID2 mRNA","volume":"12","author":"Cheng","year":"2019","journal-title":"OncoTargets Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1002\/hep.28885","article-title":"METTL14 suppresses the metastatic potential of hepatocellular carcinoma by modulating N6-methyladenosine-dependent primary MicroRNA processing","volume":"65","author":"Ma","year":"2017","journal-title":"Hepatology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1186\/s12943-019-1065-4","article-title":"METTL3-mediated N6-methyladenosine modification is critical for epithelial-mesenchymal transition and metastasis of gastric cancer","volume":"18","author":"Yue","year":"2019","journal-title":"Mol. Cancer"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/s13105-019-00690-8","article-title":"ALKBH5 promotes invasion and metastasis of gastric cancer by decreasing methylation of the lncRNA NEAT1","volume":"75","author":"Zhang","year":"2019","journal-title":"J. Physiol. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1159\/000491915","article-title":"ALKBH5 inhibits pancreatic Cancer motility by decreasing long non-coding RNA KCNK15-AS1 methylation","volume":"48","author":"He","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.stem.2017.11.016","article-title":"METTL14 inhibits hematopoietic stem\/progenitor differentiation and promotes leukemogenesis via mRNA m6A modification","volume":"22","author":"Weng","year":"2018","journal-title":"Cell Stem Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3752","DOI":"10.18632\/oncotarget.23365","article-title":"Downregulation of N6-methyladenosine binding YTHDF2 protein mediated by miR-493-3p suppresses prostate cancer by elevating N6-methyladenosine levels","volume":"9","author":"Li","year":"2018","journal-title":"Oncotarget"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lobo, J., Barros-Silva, D., Henrique, R., and Jer\u00f3nimo, C. (2018). The emerging role of epitranscriptomics in cancer: Focus on urological tumors. Genes, 9.","DOI":"10.3390\/genes9110552"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1002\/gcc.22340","article-title":"NCOA2 is a candidate target gene of 8q gain associated with clinically aggressive prostate cancer","volume":"55","author":"Silva","year":"2016","journal-title":"Genes Chromosom. Cancer"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0302-2838(01)00030-6","article-title":"Gain in chromosome 8q correlates with early progression in hormonal treated prostate cancer","volume":"41","author":"Steiner","year":"2002","journal-title":"Eur. Urol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"13827","DOI":"10.1038\/s41598-018-32310-8","article-title":"The m 6 A-methylase complex recruits TREX and regulates mRNA export","volume":"8","author":"Lesbirel","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Cai, J., Yang, F., Zhan, H., Situ, J., Li, W., Mao, Y., and Luo, Y. (2019). RNA m 6 A Methyltransferase METTL3 Promotes The Growth Of Prostate Cancer By Regulating Hedgehog Pathway. OncoTargets Ther., 12.","DOI":"10.2147\/OTT.S226796"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1021\/acs.biochem.8b01166","article-title":"The m6A writer: Rise of a machine for growing tasks","volume":"58","author":"Balacco","year":"2018","journal-title":"Biochemistry"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-019-09865-9","article-title":"RNA m 6 A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail","volume":"10","author":"Lin","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6123","DOI":"10.1038\/s41388-019-0861-z","article-title":"KIAA1429 acts as an oncogenic factor in breast cancer by regulating CDK1 in an N6-methyladenosine-independent manner","volume":"38","author":"Qian","year":"2019","journal-title":"Oncogene"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Miao, R., Dai, C.C., Mei, L., Xu, J., Sun, S.W., Xing, Y.L., Wu, L.S., Wang, M.H., and Wei, J.F. (2020). KIAA1429 regulates cell proliferation by targeting c-Jun messenger RNA directly in gastric cancer. J. Cell Physiol.","DOI":"10.1002\/jcp.29645"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/nsmb.3462","article-title":"N 6-methyladenosine (m 6 A) recruits and repels proteins to regulate mRNA homeostasis","volume":"24","author":"Edupuganti","year":"2017","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1038\/nature14234","article-title":"N 6-methyladenosine-dependent RNA structural switches regulate RNA\u2013protein interactions","volume":"518","author":"Liu","year":"2015","journal-title":"Nature"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Han, Y., Feng, J., Xia, L., Dong, X., Zhang, X., Zhang, S., Miao, Y., Xu, Q., Xiao, S., and Zuo, Z. (2019). CVm6A: A Visualization and Exploration Database for m6As in Cell Lines. Cells, 8.","DOI":"10.3390\/cells8020168"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/s41556-019-0315-4","article-title":"The RNA N 6-methyladenosine modification landscape of human fetal tissues","volume":"21","author":"Xiao","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.bbrc.2016.08.120","article-title":"The up-regulation of long non-coding RNA CCAT2 indicates a poor prognosis for prostate cancer and promotes metastasis by affecting epithelial-mesenchymal transition","volume":"480","author":"Zheng","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/j.molcel.2019.12.005","article-title":"The dynamics of cytoplasmic mRNA metabolism","volume":"77","author":"Eisen","year":"2020","journal-title":"Mol. Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/s41556-018-0045-z","article-title":"Recognition of RNA N 6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation","volume":"20","author":"Huang","year":"2018","journal-title":"Nat. Cell Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/nprot.2014.043","article-title":"Mapping the miRNA interactome by cross-linking ligation and sequencing of hybrids (CLASH)","volume":"9","author":"Helwak","year":"2014","journal-title":"Nat. Protoc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/j.jmb.2015.08.021","article-title":"N6-methyladenosine modification in a long noncoding RNA hairpin predisposes its conformation to protein binding","volume":"428","author":"Zhou","year":"2016","journal-title":"J. Mol. Biol."},{"key":"ref_48","first-page":"2991","article-title":"Long non-coding RNA CCAT1 promotes the migration and invasion of prostate cancer PC-3 cells","volume":"22","author":"Chen","year":"2018","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/s41419-017-0241-y","article-title":"MicroRNA-27a-5p regulation by promoter methylation and MYC signaling in prostate carcinogenesis","volume":"9","author":"Duarte","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_50","first-page":"4338","article-title":"CCAT1 promotes laryngeal squamous cell carcinoma cell proliferation and invasion","volume":"8","author":"Zhuang","year":"2016","journal-title":"Am. J. Transl. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1186\/s12943-017-0725-5","article-title":"A novel mechanism of lncRNA and miRNA interaction: CCAT2 regulates miR-145 expression by suppressing its maturation process in colon cancer cells","volume":"16","author":"Yu","year":"2017","journal-title":"Mol. Cancer"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"9762","DOI":"10.1158\/0008-5472.CAN-07-2462","article-title":"MicroRNA let-7a down-regulates MYC and reverts MYC-induced growth in Burkitt lymphoma cells","volume":"67","author":"Sampson","year":"2007","journal-title":"Cancer Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/1756-9966-29-151","article-title":"miRNA-145 inhibits non-small cell lung cancer cell proliferation by targeting c-Myc","volume":"29","author":"Chen","year":"2010","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1158\/2159-8290.CD-12-0095","article-title":"The cBio cancer genomics portal: An open platform for exploring multidimensional cancer genomics data","volume":"2","author":"Cerami","year":"2012","journal-title":"Cancer Discov."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5950","DOI":"10.18632\/oncotarget.1909","article-title":"Anti-neoplastic properties of hydralazine in prostate cancer","volume":"5","author":"Sousa","year":"2014","journal-title":"Oncotarget"}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/12\/4\/771\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:11:24Z","timestamp":1760173884000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/12\/4\/771"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,25]]},"references-count":55,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4]]}},"alternative-id":["cancers12040771"],"URL":"https:\/\/doi.org\/10.3390\/cancers12040771","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,25]]}}}