{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:59:58Z","timestamp":1760237998538,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:00:00Z","timestamp":1594252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-031438","POCI-01-0145-FEDER-007274","POCI-01-0145-FEDER-016390"],"award-info":[{"award-number":["POCI-01-0145-FEDER-031438","POCI-01-0145-FEDER-007274","POCI-01-0145-FEDER-016390"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>The presence of TERT promoter (TERTp) mutations in thyroid cancer have been associated with worse prognosis features, whereas the extent and meaning of the expression and activation of TERT in thyroid tumours is still largely unknown. We analysed frozen samples from a series of benign and malignant thyroid tumours, displaying non-aggressive features and low mutational burden in order to evaluate the presence of TERTp mutations and TERT mRNA expression in these settings. In this series, TERTp mutations were found in 2%, only in malignant cases, in larger cancers, and from older patients. TERT mRNA expression was detected in both benign and malignant tumours, with increased frequencies in the malignant tumours with aggressive histotypes, larger tumours, and from older patients. In benign tumours, TERT mRNA expression was found in 17% of the follicular thyroid adenoma (FTA) with increased levels of expression in smaller tumours and associated with the presence of thyroiditis. TERTp mutations and TERT mRNA expression are correlated with worse prognosis features in malignant thyroid tumours, whereas TERT mRNA expression in the benign tumours is associated with the presence of thyroiditis.<\/jats:p>","DOI":"10.3390\/cancers12071846","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T09:45:31Z","timestamp":1594374331000},"page":"1846","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comprehensive Assessment of TERT mRNA Expression across a Large Cohort of Benign and Malignant Thyroid Tumours"],"prefix":"10.3390","volume":"12","author":[{"given":"Ana","family":"Pestana","sequence":"first","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup), 4200-135 Porto, Portugal"},{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Medical Faculty of University of Porto (FMUP), 4200-139 Porto, Portugal"}]},{"given":"Rui","family":"Batista","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup), 4200-135 Porto, Portugal"},{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Medical Faculty of University of Porto (FMUP), 4200-139 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9605-6433","authenticated-orcid":false,"given":"Ricardo","family":"Celestino","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup), 4200-135 Porto, Portugal"},{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Medical Faculty of University of Porto (FMUP), 4200-139 Porto, Portugal"},{"name":"School of Allied Health Technologies, Polytechnic of Porto, 4200-072 Porto, Portugal"}]},{"given":"Sule","family":"Canberk","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup), 4200-135 Porto, Portugal"},{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Abel Salazar Biomedical Sciences Institute (ICBAS), University of Porto, 4050-313 Porto, Portugal"}]},{"given":"Manuel","family":"Sobrinho-Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup), 4200-135 Porto, Portugal"},{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Medical Faculty of University of Porto (FMUP), 4200-139 Porto, Portugal"},{"name":"Department of Pathology, Centro Hospitalar S\u00e3o Jo\u00e3o, 4200-139 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9607-6998","authenticated-orcid":false,"given":"Paula","family":"Soares","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup), 4200-135 Porto, Portugal"},{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Medical Faculty of University of Porto (FMUP), 4200-139 Porto, Portugal"},{"name":"Department of Pathology, Medical Faculty of the University of Porto, 4200-139 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.cell.2011.02.013","article-title":"Hallmarks of cancer: The next generation","volume":"144","author":"Hanahan","year":"2011","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1126\/science.7605428","article-title":"Specific association of human telomerase activity with immortal cells and cancer","volume":"266","author":"Kim","year":"1994","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/S0959-8049(97)00062-2","article-title":"A survey of telomerase activity in human cancer","volume":"33","author":"Shay","year":"1997","journal-title":"Eur. J. Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/337331a0","article-title":"A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis","volume":"337","author":"Greider","year":"1989","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1093\/genetics\/144.4.1399","article-title":"Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes","volume":"144","author":"Lendvay","year":"1996","journal-title":"Genetics"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10712","DOI":"10.1073\/pnas.93.20.10712","article-title":"Purification of telomerase from Euplotes aediculatus: Requirement of a primer 3\u2032 overhang","volume":"93","author":"Lingner","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1093\/hmg\/8.1.137","article-title":"The human telomerase catalytic subunit hTERT: Organization of the gene and characterization of the promoter","volume":"8","author":"Cong","year":"1999","journal-title":"Hum. Mol. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.3748\/wjg.v9.i11.2395","article-title":"Correlation between expression of human telomerase subunits and telomerase activity in esophageal squamous cell carcinoma","volume":"9","author":"Li","year":"2003","journal-title":"World J. Gastroenterol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1128\/MCB.19.6.3989","article-title":"Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels","volume":"19","author":"Yi","year":"1999","journal-title":"Mol. Cell Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1126\/science.1230062","article-title":"TERT promoter mutations in familial and sporadic melanoma","volume":"339","author":"Horn","year":"2013","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1126\/science.1229259","article-title":"Highly recurrent TERT promoter mutations in human melanoma","volume":"339","author":"Huang","year":"2013","journal-title":"Science"},{"key":"ref_12","unstructured":"DeLellis, R.A., Lloyd, R.V., Heitz, P.U., and Eng, C. (2004). World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Endocrine Organs, IARC Press."},{"key":"ref_13","unstructured":"Lloyd, R., Osamura, R., Kl\u00f6ppel, G., and Rosai, J. (2017). WHO Classification of Tumours of Endocrine Organs, International Agency for Research on Cancer, IARC. [4th ed.]."},{"key":"ref_14","first-page":"2790","article-title":"Comparison of telomerase and CD44 expression as diagnostic tumour markers in lesions of the thyroid","volume":"5","author":"Aogi","year":"1999","journal-title":"Clin. Cancer Res. Off. J. Am. Assoc. Cancer Res."},{"key":"ref_15","first-page":"265","article-title":"Expression of telomerase genes in thyroid carcinoma","volume":"21","author":"Boltze","year":"2002","journal-title":"Int. J. Oncol."},{"key":"ref_16","first-page":"1483","article-title":"Human telomerase reverse transcriptase (hTERT) gene expression in thyroid neoplasms","volume":"5","author":"Saji","year":"1999","journal-title":"Clin. Cancer Res. Off. J. Am. Assoc. Cancer Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"609","DOI":"10.2174\/138920211798120853","article-title":"Genetic alterations in poorly differentiated and undifferentiated thyroid carcinomas","volume":"12","author":"Soares","year":"2011","journal-title":"Curr. Genom."},{"key":"ref_18","first-page":"715","article-title":"Quantitative measurement of telomerase reverse transcriptase, thyroglobulin and thyroid transcription factor 1 mRNAs in anaplastic thyroid carcinoma tissues and cell lines","volume":"18","author":"Takano","year":"2007","journal-title":"Oncol. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"E1550","DOI":"10.1210\/jc.2015-2690","article-title":"Association of TERT Promoter Mutation, But Not BRAF Mutation, With Increased Mortality in PTC","volume":"100","author":"George","year":"2015","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1210\/jc.2013-2383","article-title":"Frequent somatic TERT promoter mutations in thyroid cancer: Higher prevalence in advanced forms of the disease","volume":"98","author":"Landa","year":"2013","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1530\/ERC-14-0359","article-title":"Diagnostic and prognostic TERT promoter mutations in thyroid fine-needle aspiration biopsy","volume":"21","author":"Liu","year":"2014","journal-title":"Endocr.-Relat. Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Liu, T., Wang, N., Cao, J., Sofiadis, A., Dinets, A., Zedenius, J., Larsson, C., and Xu, D. (2013). The age- and shorter telomere-dependent TERT promoter mutation in follicular thyroid cell-derived carcinomas. Oncogene.","DOI":"10.1038\/onc.2013.446"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1530\/ERC-13-0210","article-title":"Highly prevalent TERT promoter mutations in aggressive thyroid cancers","volume":"20","author":"Liu","year":"2013","journal-title":"Endocr.-Relat. Cancer"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1210\/jc.2013-3734","article-title":"TERT promoter mutations are a major indicator of poor outcome in differentiated thyroid carcinomas","volume":"99","author":"Melo","year":"2014","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s00428-016-2027-5","article-title":"In Japanese patients with papillary thyroid carcinoma, TERT promoter mutation is associated with poor prognosis, in contrast to BRAF V600E mutation","volume":"469","author":"Nasirden","year":"2016","journal-title":"Virchows Arch. Int. J. Pathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1530\/ERC-15-0396","article-title":"TERT promoter mutations in thyroid cancer: A report from a Middle Eastern population","volume":"22","author":"Qasem","year":"2015","journal-title":"Endocr.-Relat. Cancer"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Boaventura, P., Batista, R., Pestana, A., Reis, M., Mendes, A., Eloy, C., Sobrinho-Simoes, M., and Soares, P. (2016). TERT promoter mutations: A genetic signature of benign and malignant thyroid tumours occurring in the context of tinea capitis irradiation. Eur. J. Endocrinol.","DOI":"10.1530\/EJE-16-0740"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1089\/thy.2017.0504","article-title":"TERT, HRAS, and EIF1AX Mutations in a Patient with Follicular Adenoma","volume":"28","author":"Topf","year":"2018","journal-title":"Thyroid. Off. J. Am. Thyroid Assoc."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wang, N., Liu, T., Sofiadis, A., Juhlin, C.C., Zedenius, J., Hoog, A., Larsson, C., and Xu, D. (2014). TERT promoter mutation as an early genetic event activating telomerase in follicular thyroid adenoma (FTA) and atypical FTA. Cancer.","DOI":"10.1002\/cncr.28800"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1530\/ERC-18-0050","article-title":"TERT aberrancies: A screening tool for malignancy in follicular thyroid tumours","volume":"25","author":"Paulsson","year":"2018","journal-title":"Endocr.-Relat. Cancer"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Hysek, M., Paulsson, J.O., Jatta, K., Shabo, I., Stenman, A., Hoog, A., Larsson, C., Zedenius, J., and Juhlin, C.C. (2019). Clinical Routine TERT Promoter Mutational Screening of Follicular Thyroid Tumours of Uncertain Malignant Potential (FT-UMPs): A Useful Predictor of Metastatic Disease. Cancers, 11.","DOI":"10.3390\/cancers11101443"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"17426","DOI":"10.1073\/pnas.1310522110","article-title":"TERT promoter mutations in bladder cancer affect patient survival and disease recurrence through modification by a common polymorphism","volume":"110","author":"Rachakonda","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Melo, M., da Rocha, A.G., Vinagre, J., Sobrinho-Simoes, M., and Soares, P. (2015). Coexistence of TERT Promoter and BRAF Mutations in Papillary Thyroid Carcinoma: Added Value in Patient Prognosis?. J. Clin. Oncol. Off. J. Am. Soc. Clin. Oncol.","DOI":"10.1200\/JCO.2014.59.4614"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1210\/jc.2016-2785","article-title":"TERT, BRAF, and NRAS in Primary Thyroid Cancer and Metastatic Disease","volume":"102","author":"Melo","year":"2017","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e2185","DOI":"10.1038\/ncomms3185","article-title":"Frequency of TERT promoter mutations in human cancers","volume":"4","author":"Vinagre","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4505","DOI":"10.1038\/sj.onc.1209487","article-title":"hTERT: A novel endogenous inhibitor of the mitochondrial cell death pathway","volume":"25","author":"Massard","year":"2006","journal-title":"Oncogene"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1002\/cncr.26500","article-title":"The association of the BRAF(V600E) mutation with prognostic factors and poor clinical outcome in papillary thyroid cancer: A meta-analysis","volume":"118","author":"Kim","year":"2012","journal-title":"Cancer"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1097\/MD.0b013e31826a9c71","article-title":"BRAF mutation in papillary thyroid cancer and its value in tailoring initial treatment: A systematic review and meta-analysis","volume":"91","author":"Tufano","year":"2012","journal-title":"Medicine"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1200\/JCO.2014.56.8253","article-title":"Association between BRAF V600E mutation and recurrence of papillary thyroid cancer","volume":"33","author":"Xing","year":"2015","journal-title":"J. Clin. Oncol. Off. J. Am. Soc. Clin. Oncol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1001\/jama.2013.3190","article-title":"Association between BRAF V600E mutation and mortality in patients with papillary thyroid cancer","volume":"309","author":"Xing","year":"2013","journal-title":"JAMA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/j.1365-2265.2004.02089.x","article-title":"BRAF mutations in an Italian cohort of thyroid cancers","volume":"61","author":"Fugazzola","year":"2004","journal-title":"Clin. Endocrinol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1507\/endocrj.K08E-208","article-title":"BRAF mutation in papillary thyroid carcinoma in a Japanese population: Its lack of correlation with high-risk clinicopathological features and disease-free survival of patients","volume":"56","author":"Ito","year":"2009","journal-title":"Endocr J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3603","DOI":"10.1210\/jc.2006-1006","article-title":"Prevalence of RET\/PTC rearrangements in thyroid papillary carcinomas: Effects of the detection methods and genetic heterogeneity","volume":"91","author":"Zhu","year":"2006","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1111\/j.1365-2559.2012.04276.x","article-title":"RET\/PTC rearrangement is prevalent in follicular Hurthle cell carcinomas","volume":"61","author":"Celestino","year":"2012","journal-title":"Histopathology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5831","DOI":"10.1002\/cam4.2467","article-title":"Spectrum of TERT promoter mutations and mechanisms of activation in thyroid cancer","volume":"8","author":"Panebianco","year":"2019","journal-title":"Cancer Med."},{"key":"ref_46","first-page":"8","article-title":"Human telomerase reverse transcriptase (hTERT) gene expression in thyroid carcinoma: Diagnostic and prognostic role","volume":"18","author":"Asaad","year":"2006","journal-title":"J. Egypt. Natl. Canc. Inst."},{"key":"ref_47","first-page":"213","article-title":"Telomerase activity in well-differentiated papillary thyroid carcinoma correlates with advanced clinical stage of the disease","volume":"14","author":"Herrera","year":"2003","journal-title":"Endocr. Pathol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1677\/ERC-06-0023","article-title":"A PCR-based expression signature of malignancy in follicular thyroid tumours","volume":"14","author":"Foukakis","year":"2007","journal-title":"Endocr.-Relat. Cancer"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1089\/thy.2018.0695","article-title":"TERT mRNA Expression as a Novel Prognostic Marker in Papillary Thyroid Carcinomas","volume":"29","author":"Tanaka","year":"2019","journal-title":"Thyroid Off. J. Am. Thyroid Assoc."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gene.2004.06.011","article-title":"Genetic and epigenetic modulation of telomerase activity in development and disease","volume":"340","author":"Liu","year":"2004","journal-title":"Gene"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"5762","DOI":"10.1158\/1078-0432.CCR-03-0389","article-title":"Human telomerase reverse transcriptase gene expression and the surgical management of suspicious thyroid tumours","volume":"10","author":"Umbricht","year":"2004","journal-title":"Clin. Cancer Res. Off. J. Am. Assoc. Cancer Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"18488","DOI":"10.1073\/pnas.1908275116","article-title":"Single-cell imaging reveals unexpected heterogeneity of telomerase reverse transcriptase expression across human cancer cell lines","volume":"116","author":"Rowland","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1001\/jamaoncol.2018.1446","article-title":"Change in Diagnostic Criteria for Noninvasive Follicular Thyroid Neoplasm With Papillarylike Nuclear Features","volume":"4","author":"Nikiforov","year":"2018","journal-title":"JAMA Oncol."},{"key":"ref_54","unstructured":"(2020, July 01). TRIzol\u00ae Reagent. Available online: https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/trizol_reagent.pdf."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1210\/jc.2005-1336","article-title":"PAX8-PPARgamma rearrangement is frequently detected in the follicular variant of papillary thyroid carcinoma","volume":"91","author":"Castro","year":"2006","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_56","first-page":"3947","article-title":"Expression of PAX8-PPAR gamma 1 rearrangements in both follicular thyroid carcinomas and adenomas","volume":"87","author":"Marques","year":"2002","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4267","DOI":"10.1210\/jc.2003-032224","article-title":"BRAF mutations are not a major event in post-Chernobyl childhood thyroid carcinomas","volume":"89","author":"Lima","year":"2004","journal-title":"J. Clin. Endocrinol. Metab."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/12\/7\/1846\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:49:17Z","timestamp":1760176157000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/12\/7\/1846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,9]]},"references-count":57,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["cancers12071846"],"URL":"https:\/\/doi.org\/10.3390\/cancers12071846","relation":{},"ISSN":["2072-6694"],"issn-type":[{"type":"electronic","value":"2072-6694"}],"subject":[],"published":{"date-parts":[[2020,7,9]]}}}