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Milett","award":["n."],"award-info":[{"award-number":["n."]}]},{"DOI":"10.13039\/100002156","name":"Israel Cancer Association USA","doi-asserted-by":"publisher","award":["20201181"],"award-info":[{"award-number":["20201181"]}],"id":[{"id":"10.13039\/100002156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>The mechanisms by which the nuclear lamina of tumor cells influences tumor growth and migration are highly disputed. Lamin A and its variant lamin C are key lamina proteins that control nucleus stiffness and chromatin conformation. Downregulation of lamin A\/C in two prototypic metastatic lines, B16F10 melanoma and E0771 breast carcinoma, facilitated cell squeezing through rigid pores, and reduced heterochromatin content. Surprisingly, both lamin A\/C knockdown cells grew poorly in 3D spheroids within soft agar, and lamin A\/C deficient cells derived from spheroids transcribed lower levels of the growth regulator Yap1. Unexpectedly, the transendothelial migration of both cancer cells in vitro and in vivo, through lung capillaries, was not elevated by lamin A\/C knockdown and their metastasis in lungs was even dramatically reduced. Our results are the first indication that reduced lamin A\/C content in distinct types of highly metastatic cancer cells does not elevate their transendothelial migration (TEM) capacity and diapedesis through lung vessels but can compromise lung metastasis at a post extravasation level.<\/jats:p>","DOI":"10.3390\/cancers13102383","type":"journal-article","created":{"date-parts":[[2021,5,14]],"date-time":"2021-05-14T10:54:22Z","timestamp":1620989662000},"page":"2383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Reduced Lamin A\/C Does Not Facilitate Cancer Cell Transendothelial Migration but Compromises Lung Metastasis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3425-4135","authenticated-orcid":false,"given":"Francesco","family":"Roncato","sequence":"first","affiliation":[{"name":"Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"given":"Ofer","family":"Regev","sequence":"additional","affiliation":[{"name":"Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"given":"Sara W.","family":"Feigelson","sequence":"additional","affiliation":[{"name":"Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6999-040X","authenticated-orcid":false,"given":"Sandeep Kumar","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5703-4525","authenticated-orcid":false,"given":"Lukasz","family":"Kaczmarczyk","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Ariel University, Kiryat Hamada, Ariel 40700, Israel"}]},{"given":"Nehora","family":"Levi","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Ariel University, Kiryat Hamada, Ariel 40700, Israel"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6397-3123","authenticated-orcid":false,"given":"Diana","family":"Drago-Garcia","sequence":"additional","affiliation":[{"name":"Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"given":"Samuel","family":"Ovadia","sequence":"additional","affiliation":[{"name":"Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"given":"Marina","family":"Kizner","sequence":"additional","affiliation":[{"name":"Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9827-0436","authenticated-orcid":false,"given":"Yoseph","family":"Addadi","sequence":"additional","affiliation":[{"name":"Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7991-4291","authenticated-orcid":false,"given":"Jo\u00e3o C.","family":"Sabino","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Yossi","family":"Ovadya","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7774-1355","authenticated-orcid":false,"given":"S\u00e9rgio F.","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7017-4861","authenticated-orcid":false,"given":"Ester","family":"Feldmesser","sequence":"additional","affiliation":[{"name":"Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot 76100, Israel"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6422-8715","authenticated-orcid":false,"given":"Gabi","family":"Gerlitz","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Ariel University, Kiryat Hamada, Ariel 40700, Israel"}]},{"given":"Ronen","family":"Alon","sequence":"additional","affiliation":[{"name":"Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ceb.2010.10.015","article-title":"Nuclear Mechanics during Cell Migration","volume":"23","author":"Friedl","year":"2011","journal-title":"Curr. 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