{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T14:45:24Z","timestamp":1770043524564,"version":"3.49.0"},"reference-count":67,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2021,9,19]],"date-time":"2021-09-19T00:00:00Z","timestamp":1632009600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006483","name":"AbbVie","doi-asserted-by":"publisher","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}],"id":[{"id":"10.13039\/100006483","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04462\/2020"],"award-info":[{"award-number":["UIDB\/04462\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PD\/BD\/128377\/2017"],"award-info":[{"award-number":["PD\/BD\/128377\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PD\/BD\/105768\/2014"],"award-info":[{"award-number":["PD\/BD\/105768\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["739572"],"award-info":[{"award-number":["739572"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Colorectal cancer (CRC) is one of the most common cancers worldwide. Although short-term cultures of tumour sections and xenotransplants have been used to determine drug efficacy, the results frequently fail to confer clinically useful information. Biomarker discovery has changed the paradigm for advanced CRC, though the presence of a biomarker does not necessarily translate into therapeutic success. To improve clinical outcomes, translational models predictive of drug response are needed. We describe a simple method for the fast establishment of CRC patient-derived explant (CRC-PDE) cultures from different carcinogenesis pathways, employing agitation-based platforms. A total of 26 CRC-PDE were established and a subset was evaluated for viability (n = 23), morphology and genetic key alterations (n = 21). CRC-PDE retained partial tumor glandular architecture and microenvironment features were partially lost over 4 weeks of culture. Key proteins (p53 and Mismatch repair) and oncogenic driver mutations of the original tumours were sustained throughout the culture. Drug challenge (n = 5) revealed differential drug response from distinct CRC-PDE cases. These findings suggest an adequate representation of the original tumour and highlight the importance of detailed model characterisation. The preservation of key aspects of the CRC microenvironment and genetics supports CRC-PDE potential applicability in pre- and co-clinical settings, as long as temporal dynamics are considered.<\/jats:p>","DOI":"10.3390\/cancers13184695","type":"journal-article","created":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T07:52:14Z","timestamp":1632124334000},"page":"4695","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Patient-Derived Explants of Colorectal Cancer: Histopathological and Molecular Analysis of Long-Term Cultures"],"prefix":"10.3390","volume":"13","author":[{"given":"Sara","family":"da Mata","sequence":"first","affiliation":[{"name":"Servi\u00e7o de Anatomia Patol\u00f3gica, Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil (IPOLFG, EPE), Rua Prof. Lima Basto, 1099-023 Lisboa, Portugal"},{"name":"NOVA Medical School, Universidade Nova de Lisboa, Campo dos M\u00e1rtires da P\u00e1tria 130, 1169-056 Lisboa, Portugal"}]},{"given":"Teresa","family":"Franchi-Mendes","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2780-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8154-0639","authenticated-orcid":false,"given":"Sofia","family":"Abreu","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2780-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"Bruno","family":"Filipe","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o em Patobiologia Molecular (UIPM), Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil (IPOLFG, EPE), Rua Prof. Lima Basto, 1099-023 Lisboa, Portugal"}]},{"given":"S\u00f3nia","family":"Morgado","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Anatomia Patol\u00f3gica, Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil (IPOLFG, EPE), Rua Prof. Lima Basto, 1099-023 Lisboa, Portugal"}]},{"given":"Marta","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Anatomia Patol\u00f3gica, Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil (IPOLFG, EPE), Rua Prof. Lima Basto, 1099-023 Lisboa, Portugal"}]},{"given":"Cristina","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Unidade de Investiga\u00e7\u00e3o em Patobiologia Molecular (UIPM), Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil (IPOLFG, EPE), Rua Prof. Lima Basto, 1099-023 Lisboa, Portugal"}]},{"given":"Ricardo","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Anatomia Patol\u00f3gica, Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil (IPOLFG, EPE), Rua Prof. Lima Basto, 1099-023 Lisboa, Portugal"},{"name":"Faculdade de Medicina da Universidade de Lisboa, Avenida Prof. Egas Moniz MB, 1649-028 Lisboa, Portugal"}]},{"given":"V\u00edtor E.","family":"Santo","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2780-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"Erwin R.","family":"Boghaert","sequence":"additional","affiliation":[{"name":"Abbvie Inc., 1 North Waukegan Road, North Chicago, IL 60064-6098, USA"}]},{"given":"Isadora","family":"Rosa","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Gastrenterologia, Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil (IPOLFG, EPE), Rua Prof. Lima Basto, 1099-023 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8926-279X","authenticated-orcid":false,"given":"Catarina","family":"Brito","sequence":"additional","affiliation":[{"name":"Instituto de Biologia Experimental e Tecnol\u00f3gica, Apartado 12, 2780-901 Oeiras, Portugal"},{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"VI64","DOI":"10.1093\/annonc\/mdt354","article-title":"Early Colon Cancer: ESMO Clinical Practice Guidelines for Diagnosis, Treatment and Follow-Up","volume":"24","author":"Labianca","year":"2013","journal-title":"Ann. 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