{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T18:03:18Z","timestamp":1770141798718,"version":"3.49.0"},"reference-count":66,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hospital da Luz Lisboa","award":["LH.INV.F2019015"],"award-info":[{"award-number":["LH.INV.F2019015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>We investigated the impactof microsatellite instability (MSI) and Epstein\u2013Barr virus (EBV) status in gastric cancer (GC), regarding response to perioperative chemotherapy (POPChT), overall survival (OS), and progression-free survival (PFS). We included 137 cases of operated GC, 51 of which were submitted to POPChT. MSI status was determined by multiplex PCR and EBV status by EBV-encoded RNA in situ hybridization. Thirty-seven (27%) cases presented as MSI-high, and seven (5.1%) were EBV+. Concerning tumor regression after POPChT, no differences were observed between the molecular subtypes, but females were more likely to respond (p = 0.062). No significant differences were found in OS or PFS between different subtypes. In multivariate analysis, age (HR 1.02, IC 95% 1.002\u20131.056, p = 0.033) and positive lymph nodes (HR 1.82, IC 95% 1.034\u20133.211, p = 0.038) were the only prognostic factors for OS. However, females with MSI-high tumors treated with POPChT demonstrated a significantly increased OS compared to females with MSS tumors (p = 0.031). In conclusion, we found a high proportion of MSI-high cases. MSI and EBV status did not influence OS or PFS either in patients submitted to POPChT or surgery alone. However, superior survival of females with MSI-high tumors suggests that sex disparities and molecular classification may influence treatment options in GC.<\/jats:p>","DOI":"10.3390\/cancers15010074","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T03:26:25Z","timestamp":1671765985000},"page":"74","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["EBV and MSI Status in Gastric Cancer: Does It Matter?"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8864-1739","authenticated-orcid":false,"given":"Catarina Neto","family":"do Nascimento","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Hospital Beatriz \u00c2ngelo, 2674-514 Loures, Portugal"}]},{"given":"Lu\u00eds","family":"Mascarenhas-Lemos","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Catholic University of Portugal, 2635-631 Rio de Mouro, Portugal"},{"name":"NOVA Medical School, Universidade NOVA de Lisboa, 1169-056 Lisboa, Portugal"},{"name":"Department of Pathology, Hospital da Luz de Lisboa, 1500-650 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8579-6543","authenticated-orcid":false,"given":"Jo\u00e3o Ricardo","family":"Silva","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Porto (FMUP), 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4862-3982","authenticated-orcid":false,"given":"Diogo Sousa","family":"Marques","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Porto (FMUP), 4200-319 Porto, Portugal"}]},{"given":"Catarina Ferreira","family":"Gouveia","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital Beatriz \u00c2ngelo, 2674-514 Loures, Portugal"}]},{"given":"Ana","family":"Faria","sequence":"additional","affiliation":[{"name":"Department of Oncology, Hospital Beatriz \u00c2ngelo, 2674-514 Loures, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0879-9430","authenticated-orcid":false,"given":"S\u00f3nia","family":"Velho","sequence":"additional","affiliation":[{"name":"Department of Dietetics and Nutrition, Hospital Beatriz \u00c2ngelo, 2674-514 Loures, Portugal"}]},{"given":"Rita","family":"Garrido","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Hospital Beatriz \u00c2ngelo, 2674-514 Loures, Portugal"}]},{"given":"Rui","family":"Maio","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Hospital Beatriz \u00c2ngelo, 2674-514 Loures, Portugal"},{"name":"Department of General Surgery, Hospital da Luz de Lisboa, 1500-650 Lisboa, Portugal"}]},{"given":"Andreia","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Oncology, Centro Hospitalar Universit\u00e1rio de S\u00e3o Jo\u00e3o (CHUSJ), 4200-319 Porto, Portugal"}]},{"given":"Patr\u00edcia","family":"Pontes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Centro Hospitalar Universit\u00e1rio de S\u00e3o Jo\u00e3o (CHUSJ), 4200-319 Porto, Portugal"}]},{"given":"Xiaogang","family":"Wen","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade and Institute of Molecular Pathology and Immunology, University of Porto (Ipatimup), 4200-135 Porto, Portugal"},{"name":"Department of Pathology, Centro Hospitalar de Santo Ant\u00f3nio, 4099-001 Porto, Portugal"}]},{"given":"Irene","family":"Gullo","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Porto (FMUP), 4200-319 Porto, Portugal"},{"name":"Department of Pathology, Centro Hospitalar Universit\u00e1rio de S\u00e3o Jo\u00e3o (CHUSJ), 4200-319 Porto, Portugal"},{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade and Institute of Molecular Pathology and Immunology, University of Porto (Ipatimup), 4200-135 Porto, Portugal"}]},{"given":"Mar\u00edlia","family":"Cravo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hospital da Luz de Lisboa, 1500-650 Lisboa, Portugal"},{"name":"Faculty of Medicine, University of Lisbon (FMUL), 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1964-1006","authenticated-orcid":false,"given":"F\u00e1tima","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Porto (FMUP), 4200-319 Porto, Portugal"},{"name":"Department of Pathology, Centro Hospitalar Universit\u00e1rio de S\u00e3o Jo\u00e3o (CHUSJ), 4200-319 Porto, Portugal"},{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade and Institute of Molecular Pathology and Immunology, University of Porto (Ipatimup), 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA. 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