{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T19:12:16Z","timestamp":1775243536057,"version":"3.50.1"},"reference-count":179,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>The chick chorioallantoic membrane (CAM), as an extraembryonic tissue layer generated by the fusion of the chorion with the vascularized allantoic membrane, is easily accessible for manipulation. Indeed, grafting tumor cells on the CAM lets xenografts\/ovografts develop in a few days for further investigations. Thus, the CAM model represents an alternative test system that is a simple, fast, and low-cost tool to study tumor growth, drug response, or angiogenesis in vivo. Recently, a new era for the CAM model in immune-oncology-based drug discovery has been opened up. Although there are many advantages offering extraordinary and unique applications in cancer research, it has also disadvantages and limitations. This review will discuss the pros and cons with experts in the field.<\/jats:p>","DOI":"10.3390\/cancers15010191","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T02:52:21Z","timestamp":1672282341000},"page":"191","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["The CAM Model\u2014Q&amp;A with Experts"],"prefix":"10.3390","volume":"15","author":[{"given":"Dagmar","family":"Fischer","sequence":"first","affiliation":[{"name":"Division of Pharmaceutical Technology, Department of Chemistry and Pharmacy, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg, 91058 Erlangen, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9322-1659","authenticated-orcid":false,"given":"Georg","family":"Fluegen","sequence":"additional","affiliation":[{"name":"Department of General, Visceral, Thoracic and Pediatric Surgery (A), Medical Faculty, Heinrich-Heine-University, University Hospital Duesseldorf, 40225 Duesseldorf, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1829-816X","authenticated-orcid":false,"given":"Paul","family":"Garcia","sequence":"additional","affiliation":[{"name":"Institute for Advanced Biosciences, Research Center Universit\u00e9 Grenoble Alpes (UGA)\/Inserm U 1209\/CNRS 5309, 38700 La Tronche, France"},{"name":"R&D Department, Inovotion, 38700 La Tronche, France"}]},{"given":"Nassim","family":"Ghaffari-Tabrizi-Wizsy","sequence":"additional","affiliation":[{"name":"SFL Chicken CAM Lab, Department of Immunology, Otto Loewi Research Center, Medical University of Graz, 8010 Graz, Austria"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0595-0756","authenticated-orcid":false,"given":"Laura","family":"Gribaldo","sequence":"additional","affiliation":[{"name":"European Commission, Joint Research Centre (JRC), 21027 Ispra, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6376-3185","authenticated-orcid":false,"given":"Ruby Yun-Ju","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, National Taiwan University, Taipei 10051, Taiwan"},{"name":"Graduate Institute of Oncology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan"},{"name":"Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119077, Singapore"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8844-3583","authenticated-orcid":false,"given":"Volker","family":"Rasche","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, Ulm University Medical Center, 89073 Ulm, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4768-8431","authenticated-orcid":false,"given":"Domenico","family":"Ribatti","sequence":"additional","affiliation":[{"name":"Department of Translational Biomedicine and Neurosciences, University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5830-6577","authenticated-orcid":false,"given":"Xavier","family":"Rousset","sequence":"additional","affiliation":[{"name":"R&D Department, Inovotion, 38700 La Tronche, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8521-2904","authenticated-orcid":false,"given":"Marta Texeira","family":"Pinto","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Ipatimup\u2014Instituto de Patologia e Imunologia Molecular da Universidade do Porto, 4200-135 Porto, Portugal"}]},{"given":"Jean","family":"Viallet","sequence":"additional","affiliation":[{"name":"R&D Department, Inovotion, 38700 La Tronche, France"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"R&D Department, Inovotion, 38700 La Tronche, France"}]},{"given":"Regine","family":"Schneider-Stock","sequence":"additional","affiliation":[{"name":"Experimental Tumorpathology, Institute of Pathology, Universit\u00e4tsklinikum Erlangen, FAU Erlangen-N\u00fcrnberg, 91054 Erlangen, Germany"},{"name":"Comprehensive Cancer Center Erlangen-EMN (CCC ER-EMN), Universit\u00e4tsklinikum Erlangen, FAU Erlangen-N\u00fcrnberg, 94054 Erlangen, Germany"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,28]]},"reference":[{"key":"ref_1","first-page":"442","article-title":"An alternative in vivo model to evaluate pluripotency of patient-specific iPSCs","volume":"38","author":"Weber","year":"2021","journal-title":"ALTEX-Altern. 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