{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:31:16Z","timestamp":1760146276321,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T00:00:00Z","timestamp":1729814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Palmela Leit\u00e3o Urologia Lda","award":["LCF\/TR\/CC20\/52480003 (CTC-OD)","C630926586-00465198"],"award-info":[{"award-number":["LCF\/TR\/CC20\/52480003 (CTC-OD)","C630926586-00465198"]}]},{"name":"European Innovation Council","award":["LCF\/TR\/CC20\/52480003 (CTC-OD)","C630926586-00465198"],"award-info":[{"award-number":["LCF\/TR\/CC20\/52480003 (CTC-OD)","C630926586-00465198"]}]},{"name":"\u201cla Caixa\u201d Foundation","award":["LCF\/TR\/CC20\/52480003 (CTC-OD)","C630926586-00465198"],"award-info":[{"award-number":["LCF\/TR\/CC20\/52480003 (CTC-OD)","C630926586-00465198"]}]},{"name":"NextGenerationEU Fund","award":["LCF\/TR\/CC20\/52480003 (CTC-OD)","C630926586-00465198"],"award-info":[{"award-number":["LCF\/TR\/CC20\/52480003 (CTC-OD)","C630926586-00465198"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background: Circulating tumor cells (CTCs) may be the missing renal cell carcinoma (RCC) biomarker. No-touch (NT) resection has shown benefit in several tumors. Methods: A randomized controlled trial comparing CTC and circulating mesenchymal cell (CMC) release in no-touch (NT) vs. conventional (C) laparoscopic RN. Blood samples were collected at operation room arrival (S0), specimen extraction (S1), postoperative D1, and D30. CTCs were isolated and analyzed using RUBYchip\u2122. Results: Thirty-four patients were included. No significant differences were found between groups in CTC and CMC counts, count variations between time points, complications, and survival. The total circulating cell detection rates in the NT, C, and overall RCC groups were 58.3%, 80.0%, and 70.4% at S0; 41.6%, 86.7%, and 66.7% at S1; 50.0%, 64.3%, and 60.0% at D1; and 54.5%, 42.9%, and 44.0% at D30, respectively. A progressive decrease in CMCs was observed in the C group after surgery, especially at D1 (4.78 to 1.64 CMCs\/7.5 mL blood, p = 0.035). Healthy controls had no circulating cells; however, high CMC counts were found in chronic inflammation controls and oncocytoma patients, with no significant difference from RCC patients (p = 0.460). Conclusions: NT RN did not reduce circulating cell release nor improve survival compared to C RN.<\/jats:p>","DOI":"10.3390\/cancers16213601","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T07:51:29Z","timestamp":1729842689000},"page":"3601","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Randomized Controlled Trial Assessing the Release of Circulating Tumor and Mesenchymal Cells in No-Touch Radical Nephrectomy"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0075-6196","authenticated-orcid":false,"given":"Tito Palmela","family":"Leit\u00e3o","sequence":"first","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"},{"name":"Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"},{"name":"Urology Department, Hospital de Santa Maria, Centro Hospitalar Universit\u00e1rio Lisboa Norte, ULS Santa Maria, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7075-3516","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Corredeira","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Carolina","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"},{"name":"International Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal"}]},{"given":"Paulina","family":"Piairo","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal"},{"name":"RUBYnanomed Lda, 4700-314 Braga, Portugal"}]},{"given":"Miguel","family":"Miranda","sequence":"additional","affiliation":[{"name":"Urology Department, Hospital de Santa Maria, Centro Hospitalar Universit\u00e1rio Lisboa Norte, ULS Santa Maria, 1649-028 Lisboa, Portugal"}]},{"given":"Ana","family":"Cavaco","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Sandra","family":"Kucharczak","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"},{"name":"Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, P.O. Box 8905, 7491 Trondheim, Norway"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1257-2829","authenticated-orcid":false,"given":"Mar\u00edlia","family":"Antunes","sequence":"additional","affiliation":[{"name":"CEAUL\u2014Centro de Estat\u00edstica e Aplica\u00e7\u00f5es, Faculdade de Ci\u00eancias, Universidade de Lisboa, 1749-028 Lisboa, Portugal"}]},{"given":"Sara","family":"Peixoto","sequence":"additional","affiliation":[{"name":"Radiology Department, Hospital de Santa Maria, Centro Hospitalar Universit\u00e1rio Lisboa Norte, ULS Santa Maria, 1649-028 Lisboa, Portugal"}]},{"given":"Jos\u00e9 Palma","family":"dos Reis","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"},{"name":"Urology Department, Hospital de Santa Maria, Centro Hospitalar Universit\u00e1rio Lisboa Norte, ULS Santa Maria, 1649-028 Lisboa, Portugal"}]},{"given":"Tom\u00e9","family":"Lopes","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3695-6963","authenticated-orcid":false,"given":"Lorena","family":"Di\u00e9guez","sequence":"additional","affiliation":[{"name":"International Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal"},{"name":"RUBYnanomed Lda, 4700-314 Braga, Portugal"}]},{"given":"Lu\u00eds","family":"Costa","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Jo\u00e3o Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"},{"name":"Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"},{"name":"Oncology Department, Hospital de Santa Maria, Centro Hospitalar Universit\u00e1rio Lisboa Norte, ULS Santa Maria, 1649-028 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.eururo.2022.08.019","article-title":"Epidemiology of Renal Cell Carcinoma: 2022 Update","volume":"82","author":"Bukavina","year":"2022","journal-title":"Eur. 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