{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T18:32:43Z","timestamp":1771698763100,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>According to the present knowledge, this is the first report on establishing transformed root cultures of Leonotis nepetifolia after Rhizobium rhizogenes-mediated transformation. The preliminary phytochemical analysis showed differences in the content of phenols and flavonoids in transformed and nontransformed roots. The dominant compounds in the analyzed extracts were (+)-catechin (5464 and 6808 \u00b5g\/g DW), p-coumaric acid (2549 and 4907 \u00b5g\/g DW), m-coumaric acid (1508 and 2048 \u00b5g\/g DW) and rosmarinic acid (1844 and 2643 \u00b5g\/g DW) for nontransformed (LNNR) and transformed (LNTR4) roots, respectively. Initial biological studies carried out on LNNR, and LNTR4 extracts showed a cytotoxic effect on the A549 lung, HCC1937 breast and leukemia NALM-6 cell lines, antioxidants, as well as repair and protection against DNA damage induced by H2O2 in HUVEC cells. Due to the stronger effect of the LNTR4 root extract, which can be a relatively efficient and cheap source of bioactive secondary metabolites, further biological analyses are needed to discover in detail their potentially valuable biological properties.<\/jats:p>","DOI":"10.3390\/cells10051242","type":"journal-article","created":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T12:17:16Z","timestamp":1621340236000},"page":"1242","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Preliminary Phytochemical Analysis and Evaluation of the Biological Activity of Leonotis nepetifolia (L.) R. Br Transformed Roots Extracts Obtained through Rhizobium rhizogenes-Mediated Transformation"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4559-5015","authenticated-orcid":false,"given":"Tomasz","family":"Kowalczyk","sequence":"first","affiliation":[{"name":"Department of Molecular Biotechnology and Genetics, University of Lodz, 90-237 Lodz, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9987-4573","authenticated-orcid":false,"given":"Anna","family":"Merecz-Sadowska","sequence":"additional","affiliation":[{"name":"Department of Computer Science in Economics, University of Lodz, 90-214 Lodz, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7992-8343","authenticated-orcid":false,"given":"Patricia","family":"Rijo","sequence":"additional","affiliation":[{"name":"Center for Research in Biosciences &amp; Health Technologies (CBIOS), Universidade Lus\u00f3fona de Humanidades e Tecnologias, 1740-024 Lisboa, Portugal"},{"name":"Instituto de Investiga\u00e7\u00e3o do Medicamento (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9045-7287","authenticated-orcid":false,"given":"Vera M. S.","family":"Isca","sequence":"additional","affiliation":[{"name":"Center for Research in Biosciences &amp; Health Technologies (CBIOS), Universidade Lus\u00f3fona de Humanidades e Tecnologias, 1740-024 Lisboa, Portugal"},{"name":"Instituto de Investiga\u00e7\u00e3o do Medicamento (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4656-3557","authenticated-orcid":false,"given":"Laurent","family":"Picot","sequence":"additional","affiliation":[{"name":"Littoral Environnement et Soci\u00e9t\u00e9s LIENSs, La Rochelle Universit\u00e9, UMRi CNRS 7266 LIENSs, 17042 La Rochelle, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9610-9400","authenticated-orcid":false,"given":"Marzena","family":"Wielanek","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12\/16, 90-237 Lodz, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8385-7744","authenticated-orcid":false,"given":"Tomasz","family":"\u015aliwi\u0144ski","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5495-3550","authenticated-orcid":false,"given":"Przemys\u0142aw","family":"Sitarek","sequence":"additional","affiliation":[{"name":"Department of Biology and Pharmaceutical Botany, Medical University of Lodz, 90-151 Lodz, Poland"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,18]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Hairy roots culture as a source of valuable biopharmaceuticals","volume":"70","author":"Kowalczyk","year":"2016","journal-title":"Adv. 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