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The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including \u03c9-6 and \u03c9-3 fatty acids), several phospholipids (phosphatidylcholine, phosphatidylethanolamine, sphingomyelins, and plasmalogens), and mono- and triglycerides to be followed. Lipid changes occurred almost exclusively between days 1 and 7, followed by a tendency for lipidome stabilization after day 7. On average, phospholipids and longer and more unsaturated fatty acids increased up to day 7, probably related to plasma membrane fluidity. Articulation of lipidome changes with previously reported polar endometabolome profiling and with exometabolome changes reported here in the same cells, enabled important correlations to be established during hAMSC osteogenic differentiation. Our results supported hypotheses related to the dynamics of membrane remodelling, anti-oxidative mechanisms, protein synthesis, and energy metabolism. Importantly, the observation of specific up-taken or excreted metabolites paves the way for the identification of potential osteoinductive metabolites useful for optimized osteogenic protocols.<\/jats:p>","DOI":"10.3390\/cells11081257","type":"journal-article","created":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T13:39:51Z","timestamp":1649338791000},"page":"1257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Endo- and Exometabolome Crosstalk in Mesenchymal Stem Cells Undergoing Osteogenic Differentiation"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5813-5421","authenticated-orcid":false,"given":"Daniela S. C.","family":"Bispo","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Aveiro, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8119-004X","authenticated-orcid":false,"given":"Lenka","family":"Mich\u00e1lkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, University of Chemistry and Technology, 166 28 Prague, Czech Republic"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2026-9088","authenticated-orcid":false,"given":"Marlene","family":"Correia","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1812-1663","authenticated-orcid":false,"given":"Catarina S. H.","family":"Jesus","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4289-9256","authenticated-orcid":false,"given":"Iola F.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), 3810-193 Aveiro, Portugal"}]},{"given":"Brian J.","family":"Goodfellow","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), 3810-193 Aveiro, Portugal"}]},{"given":"Mariana B.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2342-3765","authenticated-orcid":false,"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3766-4364","authenticated-orcid":false,"given":"Ana M.","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, CICECO\u2014Aveiro Institute of Materials (CICECO\/UA), 3810-193 Aveiro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","article-title":"Minimal criteria for defining multipotent mesenchymal stromal cells. 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