{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T01:42:51Z","timestamp":1771033371925,"version":"3.50.1"},"reference-count":75,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T00:00:00Z","timestamp":1659052800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT-Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UIDB\/04551\/2020"],"award-info":[{"award-number":["UIDB\/04551\/2020"]}]},{"name":"FCT-Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UIDP\/04551\/2020"],"award-info":[{"award-number":["UIDP\/04551\/2020"]}]},{"name":"FCT-Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["LA\/P\/0087\/2020"],"award-info":[{"award-number":["LA\/P\/0087\/2020"]}]},{"name":"FCT-Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PD\/BD\/113472\/2015"],"award-info":[{"award-number":["PD\/BD\/113472\/2015"]}]},{"name":"FCT-Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BPD\/86742\/2012"],"award-info":[{"award-number":["SFRH\/BPD\/86742\/2012"]}]},{"name":"FCT-Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PTDC\/BIA-FBT\/29704\/2017"],"award-info":[{"award-number":["PTDC\/BIA-FBT\/29704\/2017"]}]},{"name":"FEDER, POR Lisboa 2020","award":["UIDB\/04551\/2020"],"award-info":[{"award-number":["UIDB\/04551\/2020"]}]},{"name":"FEDER, POR Lisboa 2020","award":["UIDP\/04551\/2020"],"award-info":[{"award-number":["UIDP\/04551\/2020"]}]},{"name":"FEDER, POR Lisboa 2020","award":["LA\/P\/0087\/2020"],"award-info":[{"award-number":["LA\/P\/0087\/2020"]}]},{"name":"FEDER, POR Lisboa 2020","award":["PD\/BD\/113472\/2015"],"award-info":[{"award-number":["PD\/BD\/113472\/2015"]}]},{"name":"FEDER, POR Lisboa 2020","award":["SFRH\/BPD\/86742\/2012"],"award-info":[{"award-number":["SFRH\/BPD\/86742\/2012"]}]},{"name":"FEDER, POR Lisboa 2020","award":["PTDC\/BIA-FBT\/29704\/2017"],"award-info":[{"award-number":["PTDC\/BIA-FBT\/29704\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The simultaneous occurrence of heat stress and drought is becoming more regular as a consequence of climate change, causing extensive agricultural losses. The application of either heat or osmotic stress increase cell-wall suberization in different tissues, which may play a role in improving plant resilience. In this work, we studied how the suberization process is affected by the combination of drought and heat stress by following the expression of suberin biosynthesis genes, cell-wall suberization and the chemical composition in Arabidopsis roots. The Arabidopsis plants used in this study were at the onset of secondary root development. At this point, one can observe a developmental gradient in the main root, with primary development closer to the root tip and secondary development, confirmed by the suberized phellem, closer to the shoot. Remarkably, we found a differential response depending on the root zone. The combination of drought and heat stress increased cell wall suberization in main root segments undergoing secondary development and in lateral roots (LRs), while the main root zone, at primary development stage, was not particularly affected. We also found differences in the overall chemical composition of the cell walls in both root zones in response to combined stress. The data gathered showed that, under combined drought and heat stress, Arabidopsis roots undergo differential cell wall remodeling depending on developmental stage, with modifications in the biosynthesis and\/or assembly of major cell wall components.<\/jats:p>","DOI":"10.3390\/cells11152341","type":"journal-article","created":{"date-parts":[[2022,7,31]],"date-time":"2022-07-31T21:49:02Z","timestamp":1659304142000},"page":"2341","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Combined Effect of Heat and Osmotic Stress on Suberization of Arabidopsis Roots"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6608-7661","authenticated-orcid":false,"given":"Ana Rita","family":"Leal","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), GPlantS, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium"},{"name":"Center for Plant Systems Biology, VIB, Technologiepark 71, 9052 Ghent, Belgium"}]},{"given":"Joana","family":"Belo","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), GPlantS, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8656-2060","authenticated-orcid":false,"given":"Tom","family":"Beeckman","sequence":"additional","affiliation":[{"name":"Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium"},{"name":"Center for Plant Systems Biology, VIB, Technologiepark 71, 9052 Ghent, Belgium"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5626-0619","authenticated-orcid":false,"given":"Pedro M.","family":"Barros","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), GPlantS, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"M. Margarida","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa (ITQB NOVA), GPlantS, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,29]]},"reference":[{"key":"ref_1","unstructured":"Jacobs, C., Berglund, M., Kurnik, B., Dworak, T., Marras, S., Mereu, V., and Michetti, M. (2019). European Environment Agency Climate change adaptation in the agriculture sector in Europe. EEA Rep., 112."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"054010","DOI":"10.1088\/1748-9326\/ab154b","article-title":"The effects of climate extremes on global agricultural yields","volume":"14","author":"Vogel","year":"2019","journal-title":"Environ. Res. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1104\/pp.113.221044","article-title":"Simultaneous application of heat, drought, and virus to Arabidopsis plants reveals significant shifts in signaling networks","volume":"162","author":"Prasch","year":"2013","journal-title":"Plant Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1104\/pp.103.033431","article-title":"When defense pathways collide: The response of Arabidopsis to a combination of drought and heat stress","volume":"134","author":"Rizhsky","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1111\/j.1365-3040.2011.02445.x","article-title":"Arabidopsis growth under prolonged high temperature and water deficit: Independent or interactive effects?","volume":"35","author":"Vile","year":"2012","journal-title":"Plant Cell Environ."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.pbi.2018.04.002","article-title":"Molecular mechanisms of combined heat and drought stress resilience in cereals","volume":"45","author":"Lawas","year":"2018","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1104\/pp.112.210773","article-title":"Transcriptome responses to combinations of stresses in Arabidopsis","volume":"161","author":"Rasmussen","year":"2013","journal-title":"Plant Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"43215","DOI":"10.1038\/srep43215","article-title":"Comparative transcriptomic analysis reveals the roles of overlapping heat-\/drought-responsive genes in poplars exposed to high temperature and drought","volume":"7","author":"Jia","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.tplants.2005.11.002","article-title":"Abiotic stress, the field environment and stress combination","volume":"11","author":"Mittler","year":"2006","journal-title":"Trends Plant Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1111\/tpj.13557","article-title":"Differences and commonalities of plant responses to single and combined stresses","volume":"90","author":"Zhang","year":"2017","journal-title":"Plant J."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Namyslov, J., Bauriedlov\u00e1, Z., Janou\u0161kov\u00e1, J., Soukup, A., and Tylov\u00e1, E. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants, 9.","DOI":"10.3390\/plants9020201"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"de Silva, N.D.G., Murmu, J., Chabot, D., Hubbard, K., Ryser, P., Molina, I., and Rowland, O. (2021). Root Suberin Plays Important Roles in Reducing Water Loss and Sodium Uptake in Arabidopsis thaliana. Metabolites, 11.","DOI":"10.3390\/metabo11110735"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1093\/aob\/mcw047","article-title":"Asymmetrical development of root endodermis and exodermis in reaction to abiotic stresses","volume":"118","author":"Martinka","year":"2016","journal-title":"Ann. Bot."},{"key":"ref_14","first-page":"1091","article-title":"Cortex cell hydraulic conductivity, endodermal apoplastic barriers and root hydraulics change in barley (Hordeum vulgare L.) in response to a low supply of N and P","volume":"124","author":"Armand","year":"2019","journal-title":"Ann. Bot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1104\/pp.19.00507","article-title":"The Intensity of Manganese Deficiency Strongly Affects Root Endodermal Suberization and Ion Homeostasis","volume":"181","author":"Chen","year":"2019","journal-title":"Plant Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"eabd0695","DOI":"10.1126\/science.abd0695","article-title":"Coordination between microbiota and root endodermis supports plant mineral nutrient homeostasis","volume":"371","author":"Reyt","year":"2021","journal-title":"Science"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4751","DOI":"10.1093\/jxb\/ers150","article-title":"Root attributes affecting water uptake of rice (Oryza sativa) under drought","volume":"63","author":"Henry","year":"2012","journal-title":"J. Exp. Bot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4411","DOI":"10.1093\/jxb\/erp281","article-title":"Transcriptomic profiling of heat-stress response in potato periderm","volume":"60","author":"Ginzberg","year":"2009","journal-title":"J. Exp. Bot."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"180","DOI":"10.3389\/fpls.2016.00180","article-title":"Transcriptomic analysis of soil-grown Arabidopsis thaliana roots and shoots in response to a drought stress","volume":"7","author":"Rasheed","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jplph.2018.02.002","article-title":"Suberized transport barriers in Arabidopsis, barley and rice roots: From the model plant to crop species","volume":"227","author":"Kreszies","year":"2018","journal-title":"J. Plant Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1111\/pce.13675","article-title":"Seminal roots of wild and cultivated barley differentially respond to osmotic stress in gene expression, suberization, and hydraulic conductivity","volume":"43","author":"Kreszies","year":"2020","journal-title":"Plant Cell Environ."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.postharvbio.2004.05.012","article-title":"The involvement of ethylene in wound-induced suberization of potato tuber (Solanum tuberosum L.): A critical assessment","volume":"34","author":"Lulai","year":"2004","journal-title":"Postharvest Biol. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plaphy.2021.01.042","article-title":"Genome-wide comparative analysis of long-chain acyl-CoA synthetases (LACSs) gene family: A focus on identification, evolution and expression profiling related to lipid synthesis","volume":"161","author":"Ayaz","year":"2021","journal-title":"Plant Physiol. Biochem."},{"key":"ref_24","first-page":"629","article-title":"The composite water and solute transport of barley (Hordeum vulgare) roots: Effect of suberized barriers","volume":"119","author":"Ranathunge","year":"2017","journal-title":"Ann. Bot."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1111\/nph.15351","article-title":"Osmotic stress enhances suberization of apoplastic barriers in barley seminal roots: Analysis of chemical, transcriptomic and physiological responses","volume":"221","author":"Kreszies","year":"2019","journal-title":"New Phytol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e00278","DOI":"10.1002\/pld3.278","article-title":"Drought activates MYB41 orthologs and induces suberization of grapevine fine roots","volume":"4","author":"Zhang","year":"2020","journal-title":"Plant Direct"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1111\/j.1365-313X.2009.03973.x","article-title":"Two Arabidopsis 3-ketoacyl CoA synthase genes, KCS20 and KCS2\/DAISY, are functionally redundant in cuticular wax and root suberin biosynthesis, but differentially controlled by osmotic stress","volume":"60","author":"Lee","year":"2009","journal-title":"Plant J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1007\/s00425-011-1350-y","article-title":"A potato skin SSH library yields new candidate genes for suberin biosynthesis and periderm formation","volume":"233","author":"Soler","year":"2011","journal-title":"Planta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"135","DOI":"10.4161\/psb.2.3.4156","article-title":"Abscisic acid and abiotic stress signaling","volume":"2","author":"Tuteja","year":"2007","journal-title":"Plant Signal. Behav."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.cell.2015.12.021","article-title":"Adaptation of Root Function by Nutrient-Induced Plasticity of Endodermal Differentiation","volume":"164","author":"Barberon","year":"2016","journal-title":"Cell"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1111\/tpj.15691","article-title":"Translational profile of developing phellem cells in Arabidopsis thaliana roots","volume":"110","author":"Leal","year":"2022","journal-title":"Plant J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/tpj.12624","article-title":"At MYB 41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types","volume":"80","author":"Kosma","year":"2014","journal-title":"Plant J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/nph.15128","article-title":"A molecular framework to study periderm formation in Arabidopsis","volume":"219","author":"Wunderling","year":"2018","journal-title":"New Phytol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2831","DOI":"10.1104\/pp.104.045856","article-title":"LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis","volume":"136","author":"Verslues","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1093\/jexbot\/51.350.1555","article-title":"Growth of Arabidopsis thaliana seedlings under water deficit studied by control of water potential in nutrient-agar media","volume":"51","author":"Spollen","year":"2000","journal-title":"J. Exp. Bot."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"111","DOI":"10.3109\/10520299109110562","article-title":"Efficient lipid staining in plant material with sudan red 7B or fluorol [correction of fluoral] yellow 088 in polyethylene glycol-glycerol","volume":"66","author":"Brundrett","year":"1991","journal-title":"Biotech. Histochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1105\/tpc.106.048033","article-title":"The acyltransferase GPAT5 is required for the synthesis of suberin in seed coat and root of Arabidopsis","volume":"19","author":"Beisson","year":"2007","journal-title":"Plant Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1104\/pp.109.144907","article-title":"Identification of an Arabidopsis feruloyl-coenzyme A transferase required for suberin synthesis","volume":"151","author":"Molina","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3569","DOI":"10.1105\/tpc.114.129049","article-title":"ABCG transporters are required for suberin and pollen wall extracellular barriers in Arabidopsis","volume":"26","author":"Yadav","year":"2014","journal-title":"Plant Cell"},{"key":"ref_41","first-page":"303","article-title":"Fourier transform mid infrared spectroscopy applications for monitoring the structural plasticity of plant cell walls","volume":"5","author":"Acebes","year":"2014","journal-title":"Front. Plant Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.1039\/c2nj40433h","article-title":"Suberin isolation from cork using ionic liquids: Characterisation of ensuing products","volume":"36","author":"Ferreira","year":"2012","journal-title":"New J. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1806","DOI":"10.1021\/bm500201s","article-title":"Ex situ reconstitution of the plant biopolyester suberin as a film","volume":"15","author":"Garcia","year":"2014","journal-title":"Biomacromolecules"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s004250050758","article-title":"Fourier transform infrared-spectroscopic characterisation of isolated endodermal cell walls from plant roots: Chemical nature in relation to anatomical development","volume":"209","author":"Zeier","year":"1999","journal-title":"Planta"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0022-2860(99)00389-0","article-title":"The hydrogen bond network in I(\u03b2) cellulose as observed by infrared spectrometry","volume":"523","author":"Chanzy","year":"2000","journal-title":"J. Mol. Struct."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"5275","DOI":"10.1007\/s10570-017-1506-4","article-title":"Predicting the cell-wall compositions of Pinus radiata (radiata pine) wood using ATR and transmission FTIR spectroscopies","volume":"24","author":"Fahey","year":"2017","journal-title":"Cellulose"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.1469-8137.2006.01823.x","article-title":"Attenuated total reflectance spectroscopy of plant leaves: A tool for ecological and botanical studies","volume":"172","year":"2006","journal-title":"New Phytol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.cej.2015.10.114","article-title":"Transparent and flexible amorphous cellulose-acrylic hybrids","volume":"287","author":"Tran","year":"2016","journal-title":"Chem. Eng. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.4161\/psb.6.8.15793","article-title":"The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors","volume":"6","author":"Encina","year":"2011","journal-title":"Plant Signal. Behav."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s11483-012-9279-7","article-title":"Use of FT-IR Spectra and PCA to the Bulk Characterization of Cell Wall Residues of Fruits and Vegetables Along a Fraction Process","volume":"8","author":"Zdunek","year":"2013","journal-title":"Food Biophys."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3225","DOI":"10.1093\/jxb\/ert163","article-title":"The potato suberin feruloyl transferase FHT which accumulates in the phellogen is induced by wounding and regulated by abscisic and salicylic acids","volume":"64","author":"Boher","year":"2013","journal-title":"J. Exp. Bot."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"379","DOI":"10.3389\/fpls.2020.00379","article-title":"Desensitization of ABA-Signaling: The Swing from Activation to Degradation","volume":"11","author":"Ali","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1016\/j.molp.2019.08.005","article-title":"Rheostatic Control of ABA Signaling through HOS15-Mediated OST1 Degradation","volume":"12","author":"Ali","year":"2019","journal-title":"Mol. Plant"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1080\/05704920500230898","article-title":"Use of Infrared Microspectroscopy in Plant Growth and Development","volume":"40","author":"Dokken","year":"2005","journal-title":"Appl. Spectrosc. Rev."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1046\/j.1365-313X.2003.01807.x","article-title":"Classification and identification of Arabidopsis cell wall mutants using Fourier-Transform InfraRed (FT-IR) microspectroscopy","volume":"35","author":"Mouille","year":"2003","journal-title":"Plant J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"59","DOI":"10.4161\/psb.6.1.13545","article-title":"Development of Casparian strip in rice cultivars","volume":"6","author":"Cai","year":"2011","journal-title":"Plant Signal. Behav."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/nrm1746","article-title":"Growth of the plant cell wall","volume":"6","author":"Cosgrove","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3389\/fpls.2012.00121","article-title":"Cell wall mechanics and growth control in plants: The role of pectins revisited","volume":"3","author":"Peaucelle","year":"2012","journal-title":"Front. Plant Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1016\/j.jplph.2007.09.006","article-title":"Water stress and cell wall polysaccharides in the apical root zone of wheat cultivars varying in drought tolerance","volume":"165","author":"Leucci","year":"2008","journal-title":"J. Plant Physiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"771","DOI":"10.3389\/fpls.2014.00771","article-title":"Cell wall remodeling under abiotic stress","volume":"5","author":"Tenhaken","year":"2015","journal-title":"Front. Plant Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.plantsci.2006.03.013","article-title":"Identification of cell wall genes modified by a permissive high temperature in Chinese cabbage","volume":"171","author":"Yang","year":"2006","journal-title":"Plant Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1104\/pp.17.00335","article-title":"PECTIN METHYLESTERASE34 contributes to heat tolerance through its role in promoting stomatal movement","volume":"174","author":"Huang","year":"2017","journal-title":"Plant Physiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.3389\/fpls.2018.01612","article-title":"Pectin methylesterases: Cell wall remodeling proteins are required for plant response to heat stress","volume":"9","author":"Wu","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1093\/jxb\/erv488","article-title":"The impact of abiotic factors on cellulose synthesis","volume":"67","author":"Wang","year":"2016","journal-title":"J. Exp. Bot."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1105\/tpc.108.065334","article-title":"Pausing of Golgi Bodies on Microtubules Regulates Secretion of Cellulose Synthase Complexes in Arabidopsis","volume":"21","author":"Crowell","year":"2009","journal-title":"Plant Cell"},{"key":"ref_66","first-page":"797","article-title":"Arabidopsis cortical microtubules position cellulose synthase delivery to the plasma membrane and interact with cellulose synthase trafficking compartments","volume":"11","author":"Gutierrez","year":"2009","journal-title":"Nat. Publ. Gr."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1111\/j.1365-313X.2011.04552.x","article-title":"Cortical microtubules optimize cell-wall crystallinity to drive unidirectional growth in Arabidopsis","volume":"66","author":"Fujita","year":"2011","journal-title":"Plant J."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s00709-011-0332-z","article-title":"The missing link: Do cortical microtubules define plasma membrane nanodomains that modulate cellulose biosynthesis?","volume":"249","author":"Fujita","year":"2012","journal-title":"Protoplasma"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.carbpol.2012.05.015","article-title":"Heat stress causes alterations in the cell-wall polymers and anatomy of coffee leaves (Coffea arabica L.)","volume":"93","author":"Lima","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Guerriero, G., Legay, S., and Hausman, J. (2014). Alfalfa Cellulose Synthase Gene Expression under Abiotic Stress: A Hitchhiker\u2019s Guide to RT-qPCR Normalization. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0103808"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3389\/fpls.2012.00045","article-title":"Mass spectrometry for characterizing plant cell wall polysaccharides","volume":"3","author":"Bauer","year":"2012","journal-title":"Front. Plant Sci."},{"key":"ref_72","first-page":"8","article-title":"Quantitation of Aliphatic Suberin in Quercus suber L. Cork by FTIR Spectroscopy","volume":"57","author":"Lopes","year":"2000","journal-title":"Biopolym. Orig. Res. Biomol."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Zhou, X., Ding, D., Ma, J., Ji, Z., Zhang, X., and Xu, F. (2015). Ultrastructure and Topochemistry of Plant Cell Wall by Transmission Electron Microscopy. The Transmission Electron Microscope-Theory and Applications, InTech.","DOI":"10.5772\/60752"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"2643","DOI":"10.1016\/j.phytochem.2005.09.027","article-title":"Apoplastic polyesters in Arabidopsis surface tissues-A typical suberin and a particular cutin","volume":"66","author":"Franke","year":"2005","journal-title":"Phytochemistry"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Ezquer, I., Salameh, I., Colombo, L., and Kalaitzis, P. (2020). Plant Cell Walls Tackling Climate Change: Biotechnological Strategies to Improve Crop Adaptations and Photosynthesis in Response to Global Warming. Plants, 9.","DOI":"10.3390\/plants9020212"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/15\/2341\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:59:35Z","timestamp":1760140775000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/11\/15\/2341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,29]]},"references-count":75,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["cells11152341"],"URL":"https:\/\/doi.org\/10.3390\/cells11152341","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,29]]}}}