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Imaging techniques are the standard for monitoring the therapy response in PDAC, but these techniques have considerable limits, including delayed disease progression detection and difficulty in distinguishing benign from malignant lesions. Extracellular vesicle (EV) liquid biopsy is an emerging diagnosis modality. Nonetheless, the majority of research for EV-based diagnosis relies on point analyses of EVs at specified times, while longitudinal EV population studies before and during therapeutic interventions remain largely unexplored. (2) Methods: We analyzed plasma EV protein composition at diagnosis and throughout PDAC therapy. (3) Results: We found that IgG is linked with the diagnosis of PDAC and the patient\u2019s response to therapy, and that the IgG+ EV population increases with disease progression and reduces with treatment response. Importantly, this covers PDAC patients devoid of the standard PDAC seric marker CA19.9 expression. We also observed that IgG is bound to EVs via the tumor antigen MAGE B1, and that this is independent of the patient\u2019s inflammatory condition and IgG seric levels. (4) Conclusions: We here propose that a population analysis of IgG+ EVs in PDAC plasma represents a novel method to supplement the monitoring of the PDAC treatment response.<\/jats:p>","DOI":"10.3390\/cells11182800","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T04:18:32Z","timestamp":1662610712000},"page":"2800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["IgG+ Extracellular Vesicles Measure Therapeutic Response in Advanced Pancreatic Cancer"],"prefix":"10.3390","volume":"11","author":[{"given":"Nuno","family":"Couto","sequence":"first","affiliation":[{"name":"Champalimaud Physiology and Cancer Programme, Champalimaud Foundation, 1400-038 Lisbon, Portugal"},{"name":"Digestive Unit, Champalimaud Clinical Centre, Champalimaud Foundation, 1400-038 Lisbon, Portugal"}]},{"given":"Julia","family":"Elzanowska","sequence":"additional","affiliation":[{"name":"Champalimaud Physiology and Cancer Programme, Champalimaud Foundation, 1400-038 Lisbon, Portugal"}]},{"given":"Joana","family":"Maia","sequence":"additional","affiliation":[{"name":"Champalimaud Physiology and Cancer Programme, Champalimaud Foundation, 1400-038 Lisbon, Portugal"}]},{"given":"Silvia","family":"Batista","sequence":"additional","affiliation":[{"name":"Champalimaud Physiology and Cancer Programme, Champalimaud Foundation, 1400-038 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0040-9474","authenticated-orcid":false,"given":"Catarina Esteves","family":"Pereira","sequence":"additional","affiliation":[{"name":"Champalimaud Physiology and Cancer Programme, Champalimaud Foundation, 1400-038 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7763-3637","authenticated-orcid":false,"given":"Hans Christian","family":"Beck","sequence":"additional","affiliation":[{"name":"Centre for Clinical Proteomics, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Sdr. Boulevard 29, DK-5000 Odense, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0657-1907","authenticated-orcid":false,"given":"Ana Sofia","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Computational and Experimental Biology Group, iNOVA4Health, NOVA MedicalSchool|Faculdade de Ci\u00eancias M\u00e9dicas, NMS|FCM, Universidade Nova de Lisboa, 1150-199 Lisbon, Portugal"}]},{"given":"Maria Carolina","family":"Strano Moraes","sequence":"additional","affiliation":[{"name":"Champalimaud Physiology and Cancer Programme, Champalimaud Foundation, 1400-038 Lisbon, Portugal"}]},{"given":"Carlos","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Digestive Unit, Champalimaud Clinical Centre, Champalimaud Foundation, 1400-038 Lisbon, Portugal"}]},{"given":"Manuela","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Mathematics and CIMA-Center for Research on Mathematics and Its Applications, University of \u00c9vora, 7004-516 Evora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6353-2616","authenticated-orcid":false,"given":"Rune","family":"Matthiesen","sequence":"additional","affiliation":[{"name":"Computational and Experimental Biology Group, iNOVA4Health, NOVA MedicalSchool|Faculdade de Ci\u00eancias M\u00e9dicas, NMS|FCM, Universidade Nova de Lisboa, 1150-199 Lisbon, Portugal"}]},{"given":"Bruno","family":"Costa-Silva","sequence":"additional","affiliation":[{"name":"Champalimaud Physiology and Cancer Programme, Champalimaud Foundation, 1400-038 Lisbon, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA A Cancer J. 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