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NM2A is fundamental for cell adhesion and motility, playing important functions in different stages of development and during the progression of viral and bacterial infections. Phosphorylation events regulate the activity and the cellular localization of NM2A. We previously identified the tyrosine phosphorylation of residue 158 (pTyr158) in the motor domain of the NM2A heavy chain. This phosphorylation can be promoted by Listeria monocytogenes infection of epithelial cells and is dependent on Src kinase; however, its molecular role is unknown. Here, we show that the status of pTyr158 defines cytoskeletal organization, affects the assembly\/disassembly of focal adhesions, and interferes with cell migration. Cells overexpressing a non-phosphorylatable NM2A variant or expressing reduced levels of Src kinase display increased stress fibers and larger focal adhesions, suggesting an altered contraction status consistent with the increased NM2A activity that we also observed. We propose NM2A pTyr158 as a novel layer of regulation of actomyosin cytoskeleton organization.<\/jats:p>","DOI":"10.3390\/cells12141871","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T01:35:16Z","timestamp":1689644116000},"page":"1871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Src-Dependent NM2A Tyrosine Phosphorylation Regulates Actomyosin Remodeling"],"prefix":"10.3390","volume":"12","author":[{"given":"Cl\u00e1udia","family":"Brito","sequence":"first","affiliation":[{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IBMC, Instituto de Biologia Celular e Molecular, 4200-135 Porto, Portugal"},{"name":"MCBiology PhD Program\u2013Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar-ICBAS, University of Porto, 4050-313 Porto, Portugal"}]},{"given":"Joana M.","family":"Pereira","sequence":"additional","affiliation":[{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IBMC, Instituto de Biologia Celular e Molecular, 4200-135 Porto, Portugal"},{"name":"MCBiology PhD Program\u2013Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar-ICBAS, University of Porto, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3777-825X","authenticated-orcid":false,"given":"Francisco S.","family":"Mesquita","sequence":"additional","affiliation":[{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IBMC, Instituto de Biologia Celular e Molecular, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4001-1332","authenticated-orcid":false,"given":"Didier","family":"Cabanes","sequence":"additional","affiliation":[{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IBMC, Instituto de Biologia Celular e Molecular, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8578-0461","authenticated-orcid":false,"given":"Sandra","family":"Sousa","sequence":"additional","affiliation":[{"name":"i3S-Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"IBMC, Instituto de Biologia Celular e Molecular, 4200-135 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1038\/nrm2786","article-title":"Non-muscle myosin II takes centre stage in cell adhesion and migration","volume":"10","author":"Ma","year":"2009","journal-title":"Nat. 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