{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T05:36:07Z","timestamp":1769060167588,"version":"3.49.0"},"reference-count":58,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:00:00Z","timestamp":1690761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MEC Spain","award":["CT2004-03005"],"award-info":[{"award-number":["CT2004-03005"]}]},{"name":"MEC Spain","award":["PC07-018"],"award-info":[{"award-number":["PC07-018"]}]},{"name":"MEC Spain","award":["UIDP\/00772\/2020"],"award-info":[{"award-number":["UIDP\/00772\/2020"]}]},{"name":"MEC Spain","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}]},{"name":"PCTI Asturias","award":["CT2004-03005"],"award-info":[{"award-number":["CT2004-03005"]}]},{"name":"PCTI Asturias","award":["PC07-018"],"award-info":[{"award-number":["PC07-018"]}]},{"name":"PCTI Asturias","award":["UIDP\/00772\/2020"],"award-info":[{"award-number":["UIDP\/00772\/2020"]}]},{"name":"PCTI Asturias","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}]},{"name":"Instituto Universitario de Oncolog\u00eda del Principado de Asturias, Obra Social Cajastur","award":["CT2004-03005"],"award-info":[{"award-number":["CT2004-03005"]}]},{"name":"Instituto Universitario de Oncolog\u00eda del Principado de Asturias, Obra Social Cajastur","award":["PC07-018"],"award-info":[{"award-number":["PC07-018"]}]},{"name":"Instituto Universitario de Oncolog\u00eda del Principado de Asturias, Obra Social Cajastur","award":["UIDP\/00772\/2020"],"award-info":[{"award-number":["UIDP\/00772\/2020"]}]},{"name":"Instituto Universitario de Oncolog\u00eda del Principado de Asturias, Obra Social Cajastur","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}]},{"name":"Portuguese Science and Technology Foundation (FCT)","award":["CT2004-03005"],"award-info":[{"award-number":["CT2004-03005"]}]},{"name":"Portuguese Science and Technology Foundation (FCT)","award":["PC07-018"],"award-info":[{"award-number":["PC07-018"]}]},{"name":"Portuguese Science and Technology Foundation (FCT)","award":["UIDP\/00772\/2020"],"award-info":[{"award-number":["UIDP\/00772\/2020"]}]},{"name":"Portuguese Science and Technology Foundation (FCT)","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The comet assay in Drosophila has been used in the last few years to study DNA damage responses (DDR) in different repair-mutant strains and to compare them to analyze DNA repair. We have used this approach to study interactions between DNA repair pathways in vivo. Additionally, we have implemented an ex vivo comet assay, in which nucleoids from treated and untreated cells were incubated ex vivo with cell-free protein extracts from individuals with distinct repair capacities. Four strains were used: wild-type OregonK (OK), nucleotide excision repair mutant mus201, dmPolQ protein mutant mus308, and the double mutant mus201;mus308. Methyl methanesulfonate (MMS) was used as a genotoxic agent. Both approaches were performed with neuroblasts from third-instar larvae; they detected the effects of the NER and dmPolQ pathways on the DDR to MMS and that they act additively in this response. Additionally, the ex vivo approach quantified that mus201, mus308, and the double mutant mus201;mus308 strains presented, respectively, 21.5%, 52.9%, and 14.8% of OK strain activity over MMS-induced damage. Considering the homology between mammals and Drosophila in repair pathways, the detected additive effect might be extrapolated even to humans, demonstrating that Drosophila might be an excellent model to study interactions between repair pathways.<\/jats:p>","DOI":"10.3390\/cells12151979","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T09:14:40Z","timestamp":1690881280000},"page":"1979","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Comet Assay in Drosophila: A Tool to Study Interactions between DNA Repair Systems in DNA Damage Responses In Vivo and Ex Vivo"],"prefix":"10.3390","volume":"12","author":[{"given":"Rub\u00e9n","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Department of Functional Biology (Genetic Area), University of Oviedo, 33006 Oviedo, Spain"},{"name":"Oncology University Institute from Asturias (IUOPA), University of Oviedo, 33006 Oviedo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4461-7021","authenticated-orcid":false,"given":"Isabel","family":"Gaiv\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biotechnology and CECAV, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]},{"given":"Leticia","family":"Aguado","sequence":"additional","affiliation":[{"name":"Department of Functional Biology (Genetic Area), University of Oviedo, 33006 Oviedo, Spain"},{"name":"Oncology University Institute from Asturias (IUOPA), University of Oviedo, 33006 Oviedo, Spain"}]},{"given":"Marta","family":"Espina","sequence":"additional","affiliation":[{"name":"Department of Functional Biology (Genetic Area), University of Oviedo, 33006 Oviedo, Spain"},{"name":"Oncology University Institute from Asturias (IUOPA), University of Oviedo, 33006 Oviedo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1566-8145","authenticated-orcid":false,"given":"Jorge","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Functional Biology (Genetic Area), University of Oviedo, 33006 Oviedo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7845-3905","authenticated-orcid":false,"given":"Pablo","family":"Mart\u00ednez-Camblor","sequence":"additional","affiliation":[{"name":"Department of Biomedical Data Science, Geisel Medical School at Dartmouth, Hanover, NH 03755, USA"},{"name":"Faculty of Health Sciences, Universidad Aut\u00f3noma de Chile, Provedencia 7500912, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9046-1171","authenticated-orcid":false,"given":"L. Mar\u00eda","family":"Sierra","sequence":"additional","affiliation":[{"name":"Department of Functional Biology (Genetic Area), University of Oviedo, 33006 Oviedo, Spain"},{"name":"Oncology University Institute from Asturias (IUOPA), University of Oviedo, 33006 Oviedo, Spain"},{"name":"Institute of Sanitary Research of the Principality of Asturias, Av. del Hospital Universitario, s\/n, 33011 Oviedo, Spain"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1385\/MB:26:3:249","article-title":"The Comet assay for DNA damage and repair. Principles, applications, and limitations","volume":"26","author":"Collins","year":"2004","journal-title":"Mol. 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