{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T01:38:16Z","timestamp":1774661896036,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T00:00:00Z","timestamp":1701820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UIDP\/5006\/2020"],"award-info":[{"award-number":["UIDP\/5006\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["CEECIND\/04259\/2017"],"award-info":[{"award-number":["CEECIND\/04259\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemosensors"],"abstract":"<jats:p>This work presents the electrochemical determination of cholecalciferol (Vitamin D3) in water-organic mixtures using a glassy carbon electrode (GCE) and commercial screen-printed carbon electrodes (SPCEs). The electrocatalytic behavior of Vitamin D3 on the surface of the working electrode produced a well-defined oxidation peak at +0.95 V (vs. Ag|AgCl, 3.0 mol L\u22121) and +0.7 V (vs. Ag-SPCE pseudo-reference electrode) for the GCE and SPCE, respectively, in 0.1 M LiClO4 prepared in 50% ethanol. The nature of the organic solvent needed for the solubilization of Vitamin D3 was evaluated, together with the concentration of the supporting electrolyte, the ratio of the water-organic mixture, the voltametric parameters for the cyclic voltammetry (CV), and square-wave voltammetry (SWV) analyses. Under the optimized conditions, a linear correlation between the anodic peak current and the concentration of Vitamin D3 was obtained over the range of 0.47 to 123 \u00b5mol L\u22121 and 59.4 to 1651 \u00b5mol L\u22121 for the GCE and SPCE, respectively. The determined limits of detection (LOD) were 0.17 (GCE) and 19.4 \u00b5mol L\u22121 (SPCE). The methodology was successfully applied to commercial supplement tablets of Vitamin D3. Additionally, this work shows the possibility of using non-modified GCE and SPCE for routine analysis of Vitamin D3.<\/jats:p>","DOI":"10.3390\/chemosensors11120575","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T06:01:07Z","timestamp":1701842467000},"page":"575","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Electrochemical Sensing of Vitamin D3: A Comparative Use of Glassy Carbon and Unmodified Screen-Printed Carbon Electrodes"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6220-4955","authenticated-orcid":false,"given":"F\u00e1tima Daniela","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"LAQV-REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3950-528X","authenticated-orcid":false,"given":"Jos\u00e9 Ant\u00f3nio","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0313-6985","authenticated-orcid":false,"given":"Rui Miguel","family":"Ramos","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade do Porto, Rua do Campo Alegre, s\/n, 4169-007 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1002\/tcr.201100005","article-title":"Voltammetry of the liposoluble vitamins (A, D, e and K) in organic solvents","volume":"12","author":"Webster","year":"2012","journal-title":"Chem. 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