{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:53:29Z","timestamp":1769162009606,"version":"3.49.0"},"reference-count":65,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T00:00:00Z","timestamp":1605657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemosensors"],"abstract":"<jats:p>A sensitive electrochemical detection method for Salmonella spp. was described, based on the use of Hechtia argentea lectin immobilised on a screen-printed gold electrode. The lectin was extracted from Hechtia argentea, a plant belonging to the Bromeliaceae family. The lectin with molecular weight near 27.4 kDa showed selectivity towards D-mannose, contained on the lipopolysaccharide cell wall of Salmonella spp. Carbohydrate selectivity of the lectin was measured as a change in impedance with respect to concentration. The binding of the bacteria to the biosensor surface increased impedance with increasing concentrations of Salmonella spp., achieving a linear range of detection of 15\u20132.57 \u00d7 107 CFU mL\u22121, with a limit of detection of 5 CFU mL\u22121. Increases in impedance were measured using electrochemical impedance spectroscopy and analysed using Nyquist plots. The biosensor was applied in analysis of hen egg samples, and the results were consistent with those obtained using the official analysis methodology.<\/jats:p>","DOI":"10.3390\/chemosensors8040115","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T07:41:00Z","timestamp":1605685260000},"page":"115","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp."],"prefix":"10.3390","volume":"8","author":[{"given":"Jorge","family":"Lopez-Tellez","sequence":"first","affiliation":[{"name":"Area Academica de Quimica, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma 42184, Mexico"}]},{"given":"Irais","family":"Sanchez-Ortega","sequence":"additional","affiliation":[{"name":"Area Academica de Quimica, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma 42184, Mexico"}]},{"given":"Claudia Teresa","family":"Hornung-Leoni","sequence":"additional","affiliation":[{"name":"Herbario, Centro de Investigaciones Biologicas, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma 42184, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3803-2820","authenticated-orcid":false,"given":"Eva Maria","family":"Santos","sequence":"additional","affiliation":[{"name":"Area Academica de Quimica, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma 42184, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7992-1491","authenticated-orcid":false,"given":"Jose Manuel","family":"Miranda","sequence":"additional","affiliation":[{"name":"Laboratorio de Higiene Inspeccion y Control de Alimentos, Departamento Quimica Analitica, Nutricion y Bromatologia, Facultad de Veterinaria Pabellon, 4 p.b. Campus Universitario, Universidad de Santiago de Compostela, 27002 Lugo, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3920-9827","authenticated-orcid":false,"given":"Jose Antonio","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Area Academica de Quimica, Universidad Autonoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma 42184, Mexico"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,18]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2020, May 08). WHO Food Safety. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/food-safety."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.4315\/0362-028X.JFP-12-160","article-title":"Risk factors for microbial contamination in fruits and vegetables at the preharvest level: A systematic review","volume":"75","author":"Park","year":"2012","journal-title":"J. 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