{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T10:26:59Z","timestamp":1762338419558,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T00:00:00Z","timestamp":1607817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004488","name":"Hrvatska Zaklada za Znanost","doi-asserted-by":"publisher","award":["IP-2018-01-5475"],"award-info":[{"award-number":["IP-2018-01-5475"]}],"id":[{"id":"10.13039\/501100004488","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (Portugal)","award":["UID\/QUI\/50006\/2013"],"award-info":[{"award-number":["UID\/QUI\/50006\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemosensors"],"abstract":"<jats:p>The interaction of 4\u2032-(N,N-dimethylamino)-6-hydroxyflavylium cation with double stranded (ds-) DNA\/RNA was studied by UV\/Vis spectrophotometry, circular dichroism (CD), and also steady-state and time-resolved emission spectroscopies at neutral and weakly acidic conditions. At pH 5, the studied molecule, in its flavylium cationic form, showed considerable binding affinities (5 &lt; logKs &lt; 6) for all ds-DNA\/RNA, contrary to chalcones forms (dominant at pH 7), which did not show binding to polynucleotides. Flavylium cation intercalated into ds-DNAs at variance to dominant groove aggregation within ds-RNA, which was reported by RNA-specific bisignate induced CD spectrum (ICD) bands. The intrinsically negligible fluorescence of flavylium was strongly increased upon the addition of DNA or RNA, whereby both the fluorescence intensity and emission lifetimes of complexes differed considerably: the strongest emission increase was observed for AU-RNA (detection limit estimated to 10 nM) followed by AT-DNAs and the much weaker effect of GC-DNAs. Both fluorescence sensitivity on the ds-DNA\/RNA secondary structure and sequence-selective ICD bands make the flavylium\u2013chalcones system an intriguing pH-switchable new probe for distinguishing between various polynucleotide sequences.<\/jats:p>","DOI":"10.3390\/chemosensors8040129","type":"journal-article","created":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T20:56:57Z","timestamp":1607893017000},"page":"129","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Flavylium Dye as pH-Tunable Fluorescent and CD Probe for Double-Stranded DNA and RNA"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9914-9010","authenticated-orcid":false,"given":"Ivo","family":"Crnolatac","sequence":"first","affiliation":[{"name":"Division of Organic Chemistry and Biochemistry, Ru\u0111er Bos\u030ckovic\u0301 Institute, Bijenic\u030cka cesta 54, P.O. Box 180, HR-10002 Zagreb, Croatia"}]},{"given":"Let\u00edcia","family":"Giestas","sequence":"additional","affiliation":[{"name":"LAQV-Laborat\u00f3rio Associado para a Qu\u00edmica Verde-REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8726-8573","authenticated-orcid":false,"given":"Gordan","family":"Horvat","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry, Faculty of Science\/Chemistry, Horvatovac 102A, HR-10000 Zagreb, Croatia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1333-9076","authenticated-orcid":false,"given":"Ant\u00f3nio Jorge","family":"Parola","sequence":"additional","affiliation":[{"name":"LAQV-Laborat\u00f3rio Associado para a Qu\u00edmica Verde-REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9933-446X","authenticated-orcid":false,"given":"Ivo","family":"Piantanida","sequence":"additional","affiliation":[{"name":"Division of Organic Chemistry and Biochemistry, Ru\u0111er Bos\u030ckovic\u0301 Institute, Bijenic\u030cka cesta 54, P.O. 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