{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T05:22:32Z","timestamp":1768195352007,"version":"3.49.0"},"reference-count":75,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["FCT\/UIDB\/00617\/2020"],"award-info":[{"award-number":["FCT\/UIDB\/00617\/2020"]}]},{"name":"Portuguese Football Federation, Portugal Football School","award":["FCT\/UIDB\/00617\/2020"],"award-info":[{"award-number":["FCT\/UIDB\/00617\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Children"],"abstract":"<jats:p>Childhood obesity continues to represent a growing challenge, and it has been associated with gut microbiota dysbiosis. This study examines the gut microbiota composition in overweight and obese school children and assesses whether a 12-week multidisciplinary intervention can induce changes in the gut microbiota. The intervention, which combined recreational football and nutritional education, was implemented among 15 school children, aged 7\u201310 years, with a Body Mass Index \u2265 85th percentile. The children were assigned into two groups: Football Group (n = 9) and Nutrition and Football Group (n = 6). Faecal samples were collected at the beginning and end of the program and analysed by sequencing the 16S rRNA gene. Over the intervention, a significant decrease was found collectively for Bifidobacterium genera (p = 0.011) and for Roseburia genera in the Football Group (p = 0.021). The relative abundance of Roseburia (p = 0.002) and Roseburia faecis (p = 0.009) was negatively correlated with moderate to vigorous physical activity (MVPA), while Prevotella copri was positively correlated with MVPA (p = 0.010) and with the daily intake of protein (p = 0.008). Our findings suggest that a multidisciplinary intervention was capable of inducing limited but significant positive changes in the gut microbiota composition in overweight and obese school children.<\/jats:p>","DOI":"10.3390\/children10071242","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T21:20:14Z","timestamp":1689801614000},"page":"1242","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Physical Activity and Nutrition Education on the Gut Microbiota in Overweight and Obese Children"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5284-9618","authenticated-orcid":false,"given":"Micaela C.","family":"Morgado","sequence":"first","affiliation":[{"name":"Research Centre in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, 4200-450 Porto, Portugal"},{"name":"Portugal Football School, Portuguese Football Federation (FPF), 1495-433 Cruz Quebrada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0945-1409","authenticated-orcid":false,"given":"M\u00f3nica","family":"Sousa","sequence":"additional","affiliation":[{"name":"CINTESIS@RISE, NOVA Medical School (NMS), Faculdade de Ci\u00eancias M\u00e9dicas (FCM), Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8365-7934","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Marques","sequence":"additional","affiliation":[{"name":"CINTESIS@RISE, NOVA Medical School (NMS), Faculdade de Ci\u00eancias M\u00e9dicas (FCM), Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6062-8279","authenticated-orcid":false,"given":"Andr\u00e9 B.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Faculty of Sports Science and Physical Education, University of Coimbra, 3040-248 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8225-8667","authenticated-orcid":false,"given":"J\u00falio A.","family":"Costa","sequence":"additional","affiliation":[{"name":"Portugal Football School, Portuguese Football Federation (FPF), 1495-433 Cruz Quebrada, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6788-4555","authenticated-orcid":false,"given":"Andr\u00e9","family":"Seabra","sequence":"additional","affiliation":[{"name":"Research Centre in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, 4200-450 Porto, Portugal"},{"name":"Portugal Football School, Portuguese Football Federation (FPF), 1495-433 Cruz Quebrada, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,19]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization, Regional Office for Europe (2022). 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