{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:08:02Z","timestamp":1760918882839,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Centre of the Portuguese Oncology Institute of Porto","award":["PI86-CI-IPOP-66-2017"],"award-info":[{"award-number":["PI86-CI-IPOP-66-2017"]}]},{"name":"FCT\/MCTES (PIDDAC): LEPABE","award":["UIDB\/00511\/2020","UIDP\/00511\/2020"],"award-info":[{"award-number":["UIDB\/00511\/2020","UIDP\/00511\/2020"]}]},{"name":"ALiCE","award":["UID\/04033\/2025"],"award-info":[{"award-number":["UID\/04033\/2025"]}]},{"DOI":"10.13039\/501100024791","name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences","doi-asserted-by":"crossref","award":["LA\/P\/0126\/2020"],"award-info":[{"award-number":["LA\/P\/0126\/2020"]}],"id":[{"id":"10.13039\/501100024791","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Cancers induced by human papillomavirus are often associated with systemic inflammation and cachexia. This study aimed to determine the interference of Pennisetum glaucum oral supplementation over multi-organic dysfunction in HPV16-transgenic mice. The experimental groups included (1) wildtype (WT) mice with standard diet, (2) WT mice with 36% Pennisetum, (3) transgenic mice with standard diet, (4) transgenic mice with 29% Pennisetum, and (5) transgenic mice with 36% Pennisetum. During the 4-week experimental protocol, body weight, food and water intake, and humane endpoints were recorded. At sacrifice, blood and tissue samples were collected for analysis. Oral supplementation with millet was shown to be safe and well tolerated by both WT and transgenic mice, with no adverse effects on behavior, food or water intake, or general animal welfare. In HPV16-transgenic animals, millet supplementation was associated with an improved health status, reduced serum glucose levels, enhanced antioxidant responses, and a notable reduction in the severity of HPV-induced skin and organ lesions. Overall, Pennisetum glaucum was safe under these experimental conditions and is a promising functional food for patients suffering from systemic paraneoplastic syndromes. Longer exposure periods and doses should be evaluated experimentally before proceeding to clinical trials of Pennisetum-containing diets.<\/jats:p>","DOI":"10.3390\/cimb47100858","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T14:29:17Z","timestamp":1760711357000},"page":"858","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pennisetum glaucum (L.) Oral Supplementation Mitigates Multi-Organic Dysfunction Associated with Carcinogenesis in HPV16-Transgenic Mice"],"prefix":"10.3390","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9519-4044","authenticated-orcid":false,"given":"Paula A.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Latifa","family":"Hajri","sequence":"additional","affiliation":[{"name":"Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna 7021, Tunisia"}]},{"given":"Armando V. Pinto","family":"Moreno","sequence":"additional","affiliation":[{"name":"Research Center for Experimental and Clinical Physiology and Pharmacology (NEC), Federal University of Maranh\u00e3o (UFMA), S\u00e3o Lu\u00eds 65080-805, Brazil"}]},{"given":"Carlos E. Dias","family":"Santos","sequence":"additional","affiliation":[{"name":"Research Center for Experimental and Clinical Physiology and Pharmacology (NEC), Federal University of Maranh\u00e3o (UFMA), S\u00e3o Lu\u00eds 65080-805, Brazil"}]},{"given":"Haissa O.","family":"Brito","sequence":"additional","affiliation":[{"name":"Research Center for Experimental and Clinical Physiology and Pharmacology (NEC), Federal University of Maranh\u00e3o (UFMA), S\u00e3o Lu\u00eds 65080-805, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8605-0054","authenticated-orcid":false,"given":"Margarida M. S. M.","family":"Bastos","sequence":"additional","affiliation":[{"name":"Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Associate Laboratory in Chemical Engineering (ALiCE), Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-8373","authenticated-orcid":false,"given":"Rui","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Viral Pathology Group, Research Center of IPO-Porto (CI-IPOP) & RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO-Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC), 4200-072 Porto, Portugal"},{"name":"ICBAS\u2014Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, University of Porto, 4050-313 Porto, Portugal"},{"name":"Faculty of Medicine, University of Porto (FMUP), 4200-072 Porto, Portugal"},{"name":"Faculty of Health Sciences, Fernando Pessoa University, 4200-150 Porto, Portugal"},{"name":"Research Department, Portuguese League Against Cancer (NRNorte), 4200-172 Porto, Portugal"}]},{"given":"Soumaya","family":"Ghodbane","sequence":"additional","affiliation":[{"name":"Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna 7021, Tunisia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6707-4942","authenticated-orcid":false,"given":"Mohamed","family":"Ammari","sequence":"additional","affiliation":[{"name":"Laboratory of Integrative Physiology, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna 7021, Tunisia"},{"name":"Higher Institute of Applied Biological Sciences of Tunis, University of Tunis El Manar, Tunis 7021, Tunisia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2151-2449","authenticated-orcid":false,"given":"Rui M.","family":"Gil da Costa","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Research Center for Experimental and Clinical Physiology and Pharmacology (NEC), Federal University of Maranh\u00e3o (UFMA), S\u00e3o Lu\u00eds 65080-805, Brazil"},{"name":"Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Associate Laboratory in Chemical Engineering (ALiCE), Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal"},{"name":"Molecular Oncology and Viral Pathology Group, Research Center of IPO-Porto (CI-IPOP) & RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO-Porto)\/Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC), 4200-072 Porto, Portugal"},{"name":"Department of Pathology, State University of Maranh\u00e3o, S\u00e3o Lu\u00eds 65055-310, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5572-6317","authenticated-orcid":false,"given":"Ana I.","family":"Faustino-Rocha","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"University of \u00c9vora, School of Sciences and Technology, Department of Zootechnics, 7004-516 \u00c9vora, Portugal"},{"name":"University of \u00c9vora, Comprehensive Health Research Center (CHRC), 7004-516 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jensen, J.E., Becker, G.L., Jackson, J.B., and Rysavy, M.B. 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