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Thus, marine biofouling is a major issue and motivates the development of antifouling coatings. In this study, the performance of two commercial marine coatings, a foul-release silicone-based paint (SilRef) and an epoxy resin (EpoRef), was evaluated regarding their abilities to prevent biofilm formation by Cyanobium sp. and Pseudoalteromonas tunicata (common microfoulers). Biofilms were developed under defined hydrodynamic conditions to simulate marine settings, and the number of biofilm cells, wet weight, and thickness were monitored for 7 weeks. The biofilm structure was analyzed by confocal laser scanning microscopy (CLSM) at the end-point. Results demonstrated that EpoRef surfaces were effective in inhibiting biofilm formation at initial stages (until day 28), while SilRef surfaces showed high efficacy in decreasing biofilm formation during maturation (from day 35 onwards). Wet weight and thickness analysis, as well as CLSM data, indicate that SilRef surfaces were less prone to biofilm formation than EpoRef surfaces. Furthermore, the efficacy of SilRef surfaces may be dependent on the fouling microorganism, while the performance of EpoRef was strongly influenced by a combined effect of surface and microorganism.<\/jats:p>","DOI":"10.3390\/coatings10090893","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T07:27:33Z","timestamp":1600414053000},"page":"893","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Experimental Assessment of the Performance of Two Marine Coatings to Curb Biofilm Formation of Microfoulers"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4819-1892","authenticated-orcid":false,"given":"Sara I.","family":"Faria","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7546-9362","authenticated-orcid":false,"given":"Rita","family":"Teixeira-Santos","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8992-1097","authenticated-orcid":false,"given":"Luciana C.","family":"Gomes","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6679-4374","authenticated-orcid":false,"given":"Elisabete R.","family":"Silva","sequence":"additional","affiliation":[{"name":"BioISI\u2014Biosystems &amp; Integrative Sciences Institute, Departmento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal"},{"name":"CERENA\u2014Centro de Recursos Naturais e Ambiente, Departamento de Engenharia Civil, Instituto Superior T\u00e9cnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal"}]},{"given":"Jo\u00e3o","family":"Morais","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-2417","authenticated-orcid":false,"given":"V\u00edtor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"CIIMAR\u2014Interdisciplinar Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos, Portugal"},{"name":"FCUP\u2014Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4069-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5233-1037","authenticated-orcid":false,"given":"Filipe J. M.","family":"Mergulh\u00e3o","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100294","DOI":"10.1016\/j.mtchem.2020.100294","article-title":"Novel marine antifouling coatings inspired by corals","volume":"17","author":"Tian","year":"2020","journal-title":"Mater. Today Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1016\/j.scitotenv.2018.10.010","article-title":"Eco-friendly non-biocide-release coatings for marine biofouling prevention","volume":"650","author":"Silva","year":"2019","journal-title":"Sci. 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