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An ethanolic extract from Moringa oleifera seed residue was used as a reducing and stabilizing agent in an aqueous solution of silver nitrate. The synthesized silver nanoparticles\u2019 hydrodynamic radius, polydispersity index, and zeta-potential were evaluated by Dynamic Light Scattering. Scanning Electron Microscopy was employed to confirm the size and morphology of the nanoparticles. Synthesis of spherical particles with 127 \u00b1 24 nm was confirmed. After sintering, the product of the synthesis was analyzed by X-ray diffraction. The X-ray diffraction pattern attributed to reflections of the (111), (200), (220), and (311) planes, which are characteristic of silver nanoparticles, confirms the successful synthesis of crystalline face-centered cubic nanoparticles. The antimicrobial activity of the bionanoparticles was tested against Escherichia coli BL21(DE3) cells and compared with the effect of a Moringa oleifera seed cake extract. Herein, we show that the growth of Escherichia coli is significantly affected by the addition of the synthesized bionanoparticles. Addition of the bionanoparticles inhibited the growth and lengthened the lag phase of the bacterial culture.<\/jats:p>","DOI":"10.3390\/coatings13081439","type":"journal-article","created":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T09:57:02Z","timestamp":1692179822000},"page":"1439","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Green Synthesis and Antibacterial Activity of Silver Nanoparticles Obtained from Moringa oleifera Seed Cake"],"prefix":"10.3390","volume":"13","author":[{"given":"Nuno","family":"Coelho","sequence":"first","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7460-8543","authenticated-orcid":false,"given":"Jo\u00e3o P.","family":"Jacinto","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"Rodrigo","family":"Silva","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"given":"J\u00e9ssica C.","family":"Soares","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5567-6073","authenticated-orcid":false,"given":"Alice S.","family":"Pereira","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7398-2661","authenticated-orcid":false,"given":"Pedro","family":"Tavares","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Associate Laboratory i4HB, Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"122880","DOI":"10.1016\/j.jclepro.2020.122880","article-title":"A comprehensive review on green synthesis of nature-inspired metal nanoparticles: Mechanism, application and toxicity","volume":"272","author":"Rana","year":"2020","journal-title":"J. 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