{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T23:17:40Z","timestamp":1768259860316,"version":"3.49.0"},"reference-count":25,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,3,28]],"date-time":"2018-03-28T00:00:00Z","timestamp":1522195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Coatings"],"abstract":"<jats:p>In recent years, the population has become acutely aware of the need to conserve the world\u2019s resources. The study of new compounds produced by natural means is important in the search for alternative green solutions that act against biodeteriogenic fungi, which promote biodeterioration of built cultural heritage sites. The present paper reports new solutions, derived from Bacillus sp. CCLBH 1053 cultures, to produce lipopeptides (LPP) that can act as green biocides to promote the safeguarding of stone artwork. In the stationary phases of bacteria growth, peptone supplementation and sub-lethal heat activation improve the second cycle of sporulation, greatly enhancing LPP production. The bioactive compounds produced by Bacillus cultures suppress biodeteriogenic fungi growth on stone materials, and, hence, provide an important contribution to the development of new biocides for cultural heritage rehabilitation.<\/jats:p>","DOI":"10.3390\/coatings8040123","type":"journal-article","created":{"date-parts":[[2018,3,29]],"date-time":"2018-03-29T05:26:37Z","timestamp":1522301197000},"page":"123","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Production of Antagonistic Compounds by Bacillus sp. with Antifungal Activity against Heritage Contaminating Fungi"],"prefix":"10.3390","volume":"8","author":[{"given":"Mara","family":"Silva","sequence":"first","affiliation":[{"name":"HERCULES Laboratory, \u00c9vora University, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"}]},{"given":"T\u00e2nia","family":"Rosado","sequence":"additional","affiliation":[{"name":"HERCULES Laboratory, \u00c9vora University, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"}]},{"given":"Marina","family":"Gonzalez-P\u00e9rez","sequence":"additional","affiliation":[{"name":"HERCULES Laboratory, \u00c9vora University, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"}]},{"given":"D\u00e9bora","family":"Gobbo","sequence":"additional","affiliation":[{"name":"HERCULES Laboratory, \u00c9vora University, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6035-6656","authenticated-orcid":false,"given":"Dora","family":"Teixeira","sequence":"additional","affiliation":[{"name":"HERCULES Laboratory, \u00c9vora University, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"},{"name":"Chemistry Department, School of Sciences and Technology, \u00c9vora University, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"}]},{"given":"Ant\u00f3nio","family":"Candeias","sequence":"additional","affiliation":[{"name":"HERCULES Laboratory, \u00c9vora University, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"},{"name":"Chemistry Department, School of Sciences and Technology, \u00c9vora University, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"}]},{"given":"Ana","family":"Caldeira","sequence":"additional","affiliation":[{"name":"HERCULES Laboratory, \u00c9vora University, Largo Marqu\u00eas de Marialva 8, 7000-809 \u00c9vora, Portugal"},{"name":"Chemistry Department, School of Sciences and Technology, \u00c9vora University, Rua Rom\u00e3o Ramalho 59, 7000-671 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2018,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ibiod.2006.11.003","article-title":"Evaluation of the efficiency of water-repellent and biocide compounds against microbial colonization of mortars","volume":"60","year":"2007","journal-title":"Int. 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