{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:08:24Z","timestamp":1760058504290,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T00:00:00Z","timestamp":1744588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT\u2014Foundation for Science and Technology","doi-asserted-by":"publisher","award":["UID\/DTP\/04138\/2021"],"award-info":[{"award-number":["UID\/DTP\/04138\/2021"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Compounds"],"abstract":"<jats:p>Natural polyphenols, especially the ones in their glycosylated form like hesperidin, rutin, and anthocyanins, are the most abundant phenolic compounds in citric fruits, apples, and red fruits, respectively. They stand out for their high nutraceutical potential, with various reported properties, like antioxidant, anti-inflammatory, anticarcinogenic, and cardioprotective. Nevertheless, these compounds have low bioavailability and are rapidly excreted and released by the organism. Therefore, the main goal of this work was to obtain polyphenols with increased bioactivity by functionalizing biocompounds in fruit juices, namely, orange, apple, and red fruits. This modification was achieved via hesperidinase, an enzyme that catalyzes the hydrolysis of several natural bioactive compounds. Hesperidinase was produced with Penicillium sp. The activity and stability of the produced enzyme, in its free and immobilized form, using the sol\u2013gel method, were assessed, as well as the bioactivity of the bioprocessed juices. Moreover, after immobilizing hesperidinase in sol\u2013gel lens-shaped particles, the activity and operational stability of the bioencapsulates were evaluated by measuring the residual activity over several runs. Using the specific substrate p-NPG, \u03b2-D-glucosidase retained 31% of its activity in the second run, 22.6% in the third, and 35% in the fourth. For \u03b1-L-rhamnosidase, using the substrate p-NPR, residual activity was 31.1% in both the fourth and fifth runs. In fruit juices, the bioencapsulates exhibited residual activities around 100% in the second run, approximately 81% in the third, and around 90% in the fourth. The antioxidant and anti-inflammatory activities of the bioprocessed juices were evaluated, and an increase in the anti-inflammatory activity was observed when compared with the non-processed juices.<\/jats:p>","DOI":"10.3390\/compounds5020012","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T07:42:01Z","timestamp":1744616521000},"page":"12","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioencapsulation of Hesperidinase from Penicillium sp. Toward Biocompounds with Enhanced Bioactivity"],"prefix":"10.3390","volume":"5","author":[{"given":"Diogo F.","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal"},{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal"}]},{"given":"Ana Catarina","family":"Severo","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal"},{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5693-7861","authenticated-orcid":false,"given":"Maria H. L.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal"},{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8640","DOI":"10.1021\/jf501963a","article-title":"Utility of hesperidinase for food function research: Enzymatic digestion of botanical extracts alters cellular antioxidant capacities and anti-inflammatory properties","volume":"62","author":"Yu","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.molcatb.2012.05.005","article-title":"Selective hydrolysis by commercially available hesperidinase for isoquercitrin production","volume":"81","author":"Wang","year":"2012","journal-title":"J. Mol. Catal. 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