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The residue of the sisal, which is the result of the extraction of fibers from its leaves, represents 95% of its weight. Considering that sisal leaves have high concentrations of sapogenins and aiming at a future phytotherapeutic, in this study, the alcoholic fraction of sisal, AFS, was developed, and the sapogenins were characterized. In vitro, the cytotoxicity (MTT) and the anti-inflammatory effect of AFS (phagocytosis and hemolysis inhibition) were evaluated. In vivo, the analgesic (formalin test\u2014FT) and anti-inflammatory (paw edema test\u2014PET) activities of AFS, orally, and the cream containing AFS, topical, were analyzed. The results demonstrated that AFS contains hecogenin and tigogenin and is not cytotoxic. In vitro, 0.5, 1, and 2 mg\/mL of AFS showed anti-inflammatory activity similar to the positive control (PC). In the FT, the dose of 25 mg\/kg did not differ from the PC in the neurogenic phase (p &gt; 0.05). In the PET, 25 and 50 mg\/kg of AFS differed from the negative control (NC) (p &lt; 0.05), and the cream with AFS (5 mg\/g) showed activity similar to the PC. The therapeutic activities of AFS probably result from sapogenins. In the future, we expect to develop an anti-inflammatory from the thousands of tons of sisal waste discarded in Brazil.<\/jats:p>","DOI":"10.3390\/cosmetics11050180","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T08:09:08Z","timestamp":1728979748000},"page":"180","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a Topical Cream from the Ethanolic of Agave sisalana Residues with Anti-Inflammatory and Analgesic Properties"],"prefix":"10.3390","volume":"11","author":[{"given":"J\u00falia Amanda Rodrigues","family":"Fracasso","sequence":"first","affiliation":[{"name":"Department of Biotechnology, School of Sciences and Languages, S\u00e3o Paulo State University (UNESP), Assis 19806-900, Brazil"}]},{"given":"Myriam Emiko","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Sciences and Languages, S\u00e3o Paulo State University (UNESP), Assis 19806-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3903-2988","authenticated-orcid":false,"given":"Lu\u00edsa Taynara Silv\u00e9rio","family":"da Costa","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Sciences and Languages, S\u00e3o Paulo State University (UNESP), Assis 19806-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3623-5093","authenticated-orcid":false,"given":"Debora Barros","family":"Barbosa","sequence":"additional","affiliation":[{"name":"School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba, 1193, Jos\u00e9 Bonifacio Street, Ara\u00e7atuba 16015-050, Brazil"}]},{"given":"Bruno Ara\u00fajo","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Sciences and Languages, S\u00e3o Paulo State University (UNESP), Assis 19806-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8983-9069","authenticated-orcid":false,"given":"Wellington Ricardo","family":"Pereira Martins","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Sciences and Languages, S\u00e3o Paulo State University (UNESP), Assis 19806-900, Brazil"}]},{"given":"Nat\u00e1lia Alves","family":"Zoppe","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Sciences and Languages, S\u00e3o Paulo State University (UNESP), Assis 19806-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7333-9158","authenticated-orcid":false,"given":"Joana","family":"Marques","sequence":"additional","affiliation":[{"name":"Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-0055","authenticated-orcid":false,"given":"Maria P. M.","family":"Marques","sequence":"additional","affiliation":[{"name":"Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5258-7980","authenticated-orcid":false,"given":"Aida Moreira","family":"da Silva","sequence":"additional","affiliation":[{"name":"Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal"},{"name":"Polytechnic of Coimbra, Coimbra Agriculture School, Bencata, 3045-601 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9186-0975","authenticated-orcid":false,"given":"Maria Jo\u00e3o","family":"Barroca","sequence":"additional","affiliation":[{"name":"Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal"},{"name":"Polytechnic of Coimbra, Coimbra Agriculture School, Bencata, 3045-601 Coimbra, Portugal"}]},{"given":"Valdecir Farias","family":"Ximenes","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Sciences, Bauru 17033-360, Brazil"}]},{"given":"Jo\u00e3o Tadeu","family":"Ribeiro-Paes","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Sciences and Languages, S\u00e3o Paulo State University (UNESP), Assis 19806-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9142-0371","authenticated-orcid":false,"given":"Lucin\u00e9ia","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Sciences and Languages, S\u00e3o Paulo State University (UNESP), Assis 19806-900, Brazil"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1038\/nm.3893","article-title":"Inflammasomes: Mechanism of Action, Role in Disease, and Therapeutics","volume":"21","author":"Guo","year":"2015","journal-title":"Nat. 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