{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T10:00:43Z","timestamp":1773396043284,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2023.02939.BDANA"],"award-info":[{"award-number":["2023.02939.BDANA"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/EQU\/00102\/2020"],"award-info":[{"award-number":["UIDB\/EQU\/00102\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/EQU\/00102\/2020"],"award-info":[{"award-number":["UIDP\/EQU\/00102\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/00102\/2025"],"award-info":[{"award-number":["UID\/00102\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["DRI\/India\/0044\/2020"],"award-info":[{"award-number":["DRI\/India\/0044\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["COMPETE2030-FEDER-00708800"],"award-info":[{"award-number":["COMPETE2030-FEDER-00708800"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Recovery and Resilience Programme of the Portuguese Republic","award":["UID\/PRR\/00102\/2025"],"award-info":[{"award-number":["UID\/PRR\/00102\/2025"]}]},{"name":"Funda\u00e7\u00e3o Calouste Gulbenkian"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cosmetics"],"abstract":"<jats:p>Atopic dermatitis (AD) is associated with skin microbiota dysbiosis, particularly the overgrowth of Staphylococcus aureus (SA) and Staphylococcus epidermidis (SE), which contribute to inflammation and barrier disruption. Humulus lupulus (hop) extracts contain bioactive compounds with antimicrobial potential. This study aimed to evaluate, in vitro, the antimicrobial and anti-biofilm effects of hop extract, obtained from brewing industry waste, against SA and SE strains relevant to AD. The extract was produced using a food-grade ethanolic extraction, chemically characterised, and tested for antimicrobial activity and biofilm inhibition using standard in vitro assays. The extract contained humulone, lupulone, and xanthohumol and complied with microbiological quality requirements. The MIC was 0.129% (w\/v) for both strains; the MLC was 0.515% for SA and 1.030% for SE. Biofilm inhibition was significant for SA at MIC, whereas SE required 5\u00d7 MIC (p &lt; 0.05). Humulus lupulus extracts obtained from brewing waste demonstrate effective antimicrobial and anti-biofilm activity, supporting their potential as sustainable agents for modulating skin microbiota in AD management.<\/jats:p>","DOI":"10.3390\/cosmetics13020062","type":"journal-article","created":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:25:59Z","timestamp":1772817959000},"page":"62","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Brewing Waste to Skin Health: Microbiota-Modulating Potential of Humulus lupulus in Atopic Dermatitis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3349-3959","authenticated-orcid":false,"given":"Ana Rita","family":"Gama","sequence":"first","affiliation":[{"name":"Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"},{"name":"RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5291-212X","authenticated-orcid":false,"given":"Joana","family":"Rolo","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"},{"name":"RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2467-4769","authenticated-orcid":false,"given":"\u00cdris","family":"Amado","sequence":"additional","affiliation":[{"name":"Faculty of Sciences and Technology, Department of Chemical Engineering, CERES, University of Coimbra, 3030-790 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5959-0015","authenticated-orcid":false,"given":"Jorge F. B.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Faculty of Sciences and Technology, Department of Chemical Engineering, CERES, University of Coimbra, 3030-790 Coimbra, Portugal"}]},{"given":"Jos\u00e9","family":"Martinez-de-Oliveira","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"},{"name":"RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8247-8902","authenticated-orcid":false,"given":"Carmen","family":"Lisboa","sequence":"additional","affiliation":[{"name":"RISE-Health, Department of Pathology, Microbiology, Faculty of Medicine, University of Porto, 4200-310 Porto, Portugal"},{"name":"Department of Dermatology and Venereology, ULS S\u00e3o Jo\u00e3o, 4200-319 Porto, Portugal"}]},{"given":"Ana","family":"Palmeira-de-Oliveira","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"},{"name":"RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Labfit-HPRD Health Products Research and Development, Lda, Edif\u00edcio UBIMEDICAL Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9311-8355","authenticated-orcid":false,"given":"Rita","family":"Palmeira-de-Oliveira","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilh\u00e3, Portugal"},{"name":"RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilh\u00e3, Portugal"},{"name":"Labfit-HPRD Health Products Research and Development, Lda, Edif\u00edcio UBIMEDICAL Estrada Municipal 506, 6200-284 Covilh\u00e3, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Afshari, M., Kolackova, M., Rosecka, M., \u010celakovsk\u00e1, J., and Krejsek, J. (2024). Unraveling the Skin; a Comprehensive Review of Atopic Dermatitis, Current Understanding, and Approaches. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1361005"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/1346-8138.15664","article-title":"Basics and Recent Advances in the Pathophysiology of Atopic Dermatitis","volume":"48","author":"Nakahara","year":"2021","journal-title":"J. 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